何 欢,谢若兰,丁森旭,等.重金属镉抗性菌的筛选及促生、修复效果[J].深圳大学学报理工版,2021,38(4):374-379.[doi:10.3724/SP.J.1249.2021.04374]
HE Huan,XIE Ruolan,DING Senxu,et al.Screening of cadmium resistant bacteria and their effects on plant growth promotion and remediation in soil[J].Journal of Shenzhen University Science and Engineering,2021,38(4):374-379.[doi:10.3724/SP.J.1249.2021.04374]
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重金属镉抗性菌的筛选及促生、修复效果
何 欢1,谢若兰1,丁森旭1,余 江1,2,3

1)四川大学建筑与环境学院,四川成都610065; 2)四川大学新能源与低碳技术研究院,四川成都610065;3)四川大学宜宾产业技术研究院,四川宜宾 644010

生物材料; 镉; 镉抗性菌株; 土壤理化性质; 促生特性; 植物萃取; 重金属污染

Screening of cadmium resistant bacteria and their effects on plant growth promotion and remediation in soil
HE Huan1, XIE Ruolan1, DING Senxu1, and YU Jiang1, 2, 3

1)College of Architecture and Environment, Sichuan University, Chengdu 610065, Sichuan Province, P.R.China;2)Institute of New Energy and Low Carbon Technology, Sichuan University, Chengdu 610065, Sichuan Province, P.R.China;3)Yibin Institute of Industrial Technology, Sichuan University, Yibin 644010, Sichuan Province, P.R.China

biomaterials; Cd; Cd resistant bacteria; soil physicochemical properties; growth-promoting properties; phytoextraction; heavy metal pollution

DOI: 10.3724/SP.J.1249.2021.04374

备注

为考察微生物-植物联合体系对土壤重金属镉(Cd)污染的修复效果,通过菌株筛选与盆栽实验,筛选一株具有较高Cd耐受和促生能力的菌株,探究植物促生菌对黑麦草富集能力、土壤理化性质和重金属赋存形态的影响.研究结果显示,伯克霍尔德氏菌株YXL1呈淡黄色、杆状、边缘圆滑,平均长度为5 μm,且耐受重金属镉的质量浓度达250 mg/L.YXL1产吲哚乙酸(indole-3-acetic acid, IAA)能力为31.56 mg/L,产铁载体能力为0.89,溶磷能力为46.18 mg/L.在YXL1与黑麦草的联合修复体系中,土壤的有机质和有效磷质量浓度均显著提高,分别增加了4.08 g/kg和2.54 mg/kg; 土壤中弱酸可提取态Cd和还原态Cd的质量分数分别达16.25%和12.53%; 与仅种植黑麦草的对照组相比,联合修复体系黑麦草的富集Cd能力提高了2.76倍.研究发现,以YXL1和黑麦草联合修复体系处理Cd污染农田土壤,4 a后耕作层土壤的Cd含量降低到《土壤环境质量农用地土壤污染风险管控标准(试行)》(GB 15618—2018)规定的风险筛选值(0.3 mg/kg)以下,可实现农用地的安全利用.
In order to investigate the remediation effect of the microbial-plant joint system on soil heavy metal Cd pollution, we carry out strain screening and pot experiment to explore the effects of plant growth-promoting bacteria on the accumulation capacity of ryegrass, soil physical and chemical properties and heavy metal speciation. The results show that the microscopic morphology of Burkholderia sp.YXL1 is pale yellow, rod-shaped and smooth edge with an average length of 5 μm, and it possesses strong growth-promoting ability and can tolerate cadmium content about 250 mg/L. The capacity of IAA production, siderophore and phosphate-solubilizing substances are 31.56 mg/L, 0.89 and 46.18 mg/L, respectively. In the YXL1-ryegrass joint remediation system, the organic matter and available phosphorus content in the soil are significantly increased by 4.08 g/kg and 2.54 mg/kg, respectively. The contents of weak acid extractable Cd and reducible Cd in the soil reach 16.25% and 12.53%, respectively. Compared with the control group, the ability of ryegrass to extract Cd from the soil is 2.76 times higher than that of the ryegrass treatment. The results also show that the Cd content of the cultivated layer soil is below the risk screening value(<0.3 mg/kg)in the Soil Environmental Quality Agricultural Land Soil Pollution Risk Control Standard(GB 15618—2018)to realize the safe use of agricultural land in the YXL1- ryegrass joint remediation system after 4 years.
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