候恺玥,娄素琳,曾志勇,等.莱茵衣藻响应缺硫的环状RNA的预测与鉴定[J].深圳大学学报理工版,2020,37(3):221-223.[doi:10.3724/SP.J.1249.2020.03221]
HOU Kaiyue,LOU Sulin,ZENG Zhiyong,et al.Prediction and identification of sulfur-responding circular RNA in Chlamydomonas reinhardtii[J].Journal of Shenzhen University Science and Engineering,2020,37(3):221-223.[doi:10.3724/SP.J.1249.2020.03221]
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莱茵衣藻响应缺硫的环状RNA的预测与鉴定
候恺玥1,娄素琳1,曾志勇1,胡章立1,2,李 辉1,2

1)广东省植物表观遗传学重点实验室,广东省海洋藻类生物工程技术研究中心,深圳大学生命与海洋科学学院, 广东深圳 518060; 2)深圳市海洋藻类生物工程技术研究中心,深圳大学龙华生物产业创新研究院,广东深圳 518060

分子生物学; 真核微藻; 莱茵衣藻; 非编码核糖核酸; 环状核糖核酸; 缺硫

Prediction and identification of sulfur-responding circular RNA in Chlamydomonas reinhardtii
HOU Kaiyue1, LOU Sulin1, ZENG Zhiyong1, HU Zhangli1, 2, and LI Hui<sup>1, 2

1)Guangdong Key Laboratory of Plant Epigenetics, Guangdong Technology Research Center for Marine Algal Bioengineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, Guangdong Province, P. R. China 2)Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Longhua Innovation Institute for Biotechnology, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China

molecular biology; eukaryotic microalga; Chlamydomonas reinhardtii(C. reinhardtii); non-coding RNA; circular RNA(circRNA); sulfur deprivation

DOI: 10.3724/SP.J.1249.2020.03221

备注

环状核糖核酸(circular ribonucleic acid, circular RNA或circRNA)是一类无 5' 帽子结构及 3' poly A 尾,以共价键连接形成闭合环形结构的非编码RNA分子, 通过转录后调控的方式参与动植物细胞的众多代谢途径. 莱茵衣藻(Chlamydomonas reinhardtii, C. reinhardtii)是一种重要的真核单细胞模式微藻, 为探究其是否存在环状 RNA, 本研究通过RNA-Seq高通量测序分析正常与缺硫培养的C. reinhardtii RNA组学数据, 首次预测获得218条环状 RNA序列, 通过对比缺硫处理前后莱茵衣藻环状 RNA 序列, 获得8条差异表达的环状 RNA序列(其中,5条为表达上调、3条为表达下调). 本研究首次对单细胞真核微藻莱茵衣藻进行环状 RNA的生物信息学预测, 确认莱茵衣藻环状 RNA响应缺硫胁迫, 为下一步揭示莱茵衣藻环状 RNA参与响应莱茵衣藻缺硫的作用机制奠定基础.

Circular ribonucleic acid(circular RNA or circRNA)is a class of non-coding RNA without 5' cap-structure and 3' poly A tail, connected by covalent bonds to form a closed circular structure, and involved in numerous metabolic pathways in animal and plant cells through post-transcriptional regulations. Chlamydomonas reinhardtii(C. reinhardtii)is an important eukaryotic single-cell microalga. In this study, 218 sequences associated with circRNA were predicted for the first time in C. reinhardtii. By comparing the sequences of circRNA from sulfur-replete and sulfur-deprived C. reinhardtii, 8 differentially expressed circRNA sequences were obtained(5 of them were up-regulated and 3 were down-regulated). This is the first report on a circRNA bioinformatics prediction of C. reinhardtii, confirming that the circRNA of C. reinhardtii responds to sulfur deprived stress.

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