新型近红外花菁类光声探针用于肼检测

深圳大学医学部生物医学工程学院,广东深圳518060

生物医学工程; 光声检测; 光声成像; 花菁类分子探针; 肼检测; 近红外光谱

Novel near-infrared cyanine-based photoacoustic probe for hydrazine detection
JIANG Chao, LIN Jing, and HUANG Peng

School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China

biomedical engineering; photoacoustic detection; photoacoustic imaging; cyanine molecule probe; hydrazine detection; near infrared spectroscopy

DOI: 10.3724/SP.J.1249.2020.01033

备注

肼含量的检测在化工、环境和生命健康等领域具有重要意义.利用在近红外区域具有较强吸收的花菁类分子为母体,发展新型光声探针用于肼检测.该探针在波长808 nm处具有很强的光声信号.当探针分子与肼作用后,随着溶液中肼浓度的增高,分子探针在波长808 nm处的光密度D(808)显著下降,而550 nm处的光密度D(550)明显升高,且在肼浓度为0~5 μmol/L内, D(808)/D(550)比值随肼浓度变化具有良好的线性关系.同时,新型光声探针在808 nm处的光声信号强度P(808)也随肼浓度增加而降低,并且在肼浓度为0~10 μmol/L内,P(808)与肼浓度呈良好的线性关系,肼检测限为0.50 μmol/L.该探针合成方法简单,并能够对肼实现快速高效的光声检测,为今后在体肼的检测提供了一种新的方法.

Hydrazine detection is of great significance in the fields of chemical industry, environment, life,health, and so on. A novel photoacoustic probe is designed and synthesized for hydrazine detection, using cyanine with strong near-infrared absorbance as the core. The probe has strong photoacoustic signal at 808 nm. When the probe molecules interact with the hydrazine, the absorbance intensities of probe at 808 nm and 550 nm decrease and increase with the increase of hydrazine concentration, respectively. The optical density ratio of D(808)/D(550)in the range of 0-5 μmol/L hydrazine concentration has a good linear relationship with the change of concentration. Meanwhile, photoacoustic intensity of probe at 808 nm P(808)gradually decreases with the increase of hydrazine concentration, and shows a good linear relationship in the range of 0-10 μmol/L, with a detection limit of 0.50 μmol/L. The probe is easy to synthesis and can achieve photoacoustic detection of hydrazine rapidly and efficiently, which provids a new solution for the development of in vivo detection of hydrazine.

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