胡 浪,刘烨蓉,许伟钊,等.集胞藻缺氮驯化株的代谢组学分析[J].深圳大学学报理工版,2019,36(No.5(473-598)):564-569.[doi:10.3724/SP.J.1249.2019.05564]
HU Lang,LIU Yerong,XU Weizhao,et al.Metabonomics analysis of nitrogen deficiency-generated strains of Synechocystis sp.[J].Journal of Shenzhen University Science and Engineering,2019,36(No.5(473-598)):564-569.[doi:10.3724/SP.J.1249.2019.05564]
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集胞藻缺氮驯化株的代谢组学分析
胡 浪,刘烨蓉,许伟钊,王 璐,胡章立,王江新,雷安平

深圳大学生命与海洋科学学院,广东深圳 518060

微生物学; 集胞藻; 蓝藻; 缺氮胁迫; 实验室驯化; 代谢组

Metabonomics analysis of nitrogen deficiency-generated strains of Synechocystis sp.
HU Lang, LIU Yerong, XU Weizhao, WANG Lu, HU Zhangli, WANG Jiangxin, and LEI Anping

College of Life Sciences and Oceanography, Shenzhen University,Shenzhen 518060,Guangdong Province, P.R.China

microbiology; Synechocystis; cyanobacteria; nitrogen deficiency; experimental evolution; metabolome

DOI: 10.3724/SP.J.1249.2019.05564

备注

集胞藻Synechocystis sp.PCC6803是一种非固氮蓝藻,它是重要的模式生物,可作为实验室驯化的研究材料.实验室驯化是指生物为适应人工环境而改变其遗传性状的过程.为研究生物在胁迫环境下的适应机制,以缺氮胁迫为选择压力对野生型Synechocystis sp.PCC6803进行了长达615 d的实验室驯化,最终获得了8株缺氮驯化株.这些驯化株在缺氮条件下的比生长速率与对照株无显著差异.用气相色谱-质谱检测这些驯化株的代谢物,共检测到52种代谢物,主要为氨基酸、脂肪酸、糖类及其衍生物.根据代谢物的组成对这些藻株进行分层聚类分析,结果显示,藻株形成3个聚类,分别由比生长速率较高的驯化株、比生长速率较低的驯化株和对照株构成.实验表明,由相同方式得到的驯化株的代谢组不同,这些驯化株可能以不同的代谢模式来应对缺氮胁迫.

Synechocystis sp. PCC6803 is a non nitrogen-fixing cyanobacteria, which is an important model organism and can be used as a research material for experimental evolution. Experimental evolution refers to the process of changing biological and genetic traits of organisms to adapt to the artificial environment. To explore the adaptation mechanism of organisms under stress environment, a 615 d experimental evolution with nitrogen deficiency as the selective pressure was conducted on wild type Synechocystis sp. PCC6803 in this study. Finally, 8 nitrogen deficiency-generated strains were obtained, and the growth of nitrogen deficiency-generated strain was tested. The results showed that the specific growth rates of nitrogen deficiency-generated strains were not significantly different from that of the control strain under nitrogen deficient conditions. The metabolites of these strains were detected by gas chromatography-mass spectrometry. A total of 52 metabolites, including amino acids, fatty acids, sugars and their derivatives, were detected. Based on the composition of metabolites, these strains were analyzed by hierarchical cluster analysis. Three clusters were formed within these strains. The nitrogen deficiency-generated strains with high specific growth rate, the strains with low specific growth rate and the control strain formed different clusters. These results suggest that the nitrogen deficiency-generated strains obtained from the same way are different in composition of metabolites and may use different metabolic mechanisms to deal with the nitrogen deficiency stress.

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