1)深圳大学光电工程学院,光电子器件与系统教育部/广东省重点实验室,广东深圳 518060; 2)深圳大学信息工程学院,广东深圳 518060

光学工程; 三维成像; 多粒子; 点扩展函数; 纳米定位; 活细胞; 动态追踪

Application of DH-PSF three-dimensional dynamic multi-particle tracking method in living cell imaging
Li Heng1, 2, Chen Danni1, Yu Bin1, Niu Hanben1, and Guo Baoping1

1)College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China 2)College of Information Engineering, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China

optical engineering; three-dimensional imaging; multi-particle; point spread function; nano-posi-tioning; living cell; dynamic tracking

DOI: 10.3724/SP.J.1249.2017.05526


利用双螺旋点扩散函数三维定位的能力建立了三维多粒子追踪成像系统,成像深度可达到4 μm,定位精度达到20 nm,并在该系统上实现了对巨噬细胞内多个荧光微球的动态追踪.结果显示,细胞内荧光探针的运动范围达到7 μm,可实时获取荧光微球在细胞内的运动速率,研究表明该系统是生物学家研究细胞内分子转运和相互关系非常有用的工具.

Multi-particle tracking is one of the most challenging aspects of living cell imaging. We establish a three-dimensional multi-particle tracking imaging system based on three-dimensional positioning of double helix point spread function.The imaging depth and localization precision can achieve 4 μm and 20 nm, respectively. The result of dynamic tracking of fluorescence microspheres in Raw cells shows that the detectable trajectory range of intracellular moleculesis could be up to 7 μm and the velocity of probes could be obtained in real time. Therefore, this system is a very useful tool for studying the intermolecular interaction in living cell.