深圳大学光电工程学院,光电子器件与系统教育部/广东省重点实验室, 广东深圳 518060

光学工程; 相干反斯托克斯拉曼散射; 图像重构; 点扩展函数; 空间分辨率; 非线性光学; 拉曼散射; 信息光学

Three-dimensional spatial resolution calibration of the coherent anti-Stokes Raman scattering microscopy
Liu Wei, Liu Shuanglong, Chen Danni, and Niu Hanben

Liu Wei, Liu Shuanglong, Chen Danni, and Niu HanbenCollege of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China

optical engineering; Raman scattering microscopy" target="_blank"> coherent anti-Stokes Raman scattering microscopy; imaging reconstruction; point spread function; resolution; nonlinear optics; Raman scattering; information optics

DOI: 10.3724/SP.J.1249.2017.03272


空间分辨率是衡量相干反斯托克斯拉曼散射(coherent anti-stokes Raman scattering,CARS)显微成像系统性能的一个重要指标. 选择直径为110 nm的聚苯乙烯微球进行三维成像,根据各个点的位置数据与光谱数据形成的二维数组分别重构了系统x-y平面和x-z平面的点扩展函数,准确测试了自行搭建的CARS显微成像系统的横向空间分辨率约为484 nm,轴向空间分辨率约为3.17 μm.

The spatial resolution is one of the most important indices for measuring the performance of coherent anti-stokes Raman scattering(CARS)microscopy system. Accordingly, we acquire the three dimensional images of the polystyrene micro-balls with 110 nm diameter in order to reconstruct the point spread function of the system on x-y and x-z planes based on the two dimensional array consisting of position data of each point and spectral data. The results show that lateral and axial spatial resolutions of the homemade CARS microscopy are about 484 nm and 3.17 μm, respectively.