深圳大学学报理工版

评述了骨替代材料的生物相容性.骨替代材料分为自体骨、异体骨和人工骨替代材料.指出成骨细胞MG- 63、成纤维细胞3T3和骨髓瘤细胞U2OS是骨替代材料的主要生物环境,可作为研究骨替代材料生物相容性的常用细胞.骨替代材料生物相容性的评价方法主要有体内生物学研究和体外生物学研究.骨替代材料对所处的体内环境有重要影响,包括对细胞形态和对细胞功能的影响等.根据细胞形态特点(如细胞自溶变化、膜破裂和核聚变),可以判断材料的毒性程度,根据细胞功能(跨膜运输、细胞黏附、细胞增殖、周期、凋亡、氧化应激作用以及调节信号传导),可以判断生物材料的生物相容性.

This paper reviews the biocompatibility of bone substitute materials classified as autologous bone, allograft bone and artificial bone substitutes. Osteoblasts MG- 63, fibroblast 3T3, myeloma cells U2OS are the main biological environments of these biological materials. Therefore, these three kinds of cells are common cells for the biocompatibility research of bone substitute materials. The evaluations of the biocompatibility of bone substitute materials include in vitro and in vivo biologics. The artificial bone substitute materials have important effects on the in vivo environment. These effects include the impacts on cell morphology and cell function. The characteristics of the cell morphology such as cell autolysis changes, rupture of membranes, nuclear fusion can determine the toxicological grade of biomaterials. The cell functions including membrane transport, cell adhesion, cell proliferation, cell cycle, apoptosis, oxidative stress and regulating signal transduction can determine the biocompatibility.

引言
1 骨结构
2 骨替代及修复材料
3 骨替代高分子复合材料
4 骨替代材料在体内的细胞环境
5 细胞形态和结构与材料毒性的关系
6 骨替代材料的跨膜运输
7 细胞黏附与材料表面化学的关系
8 细胞增殖、周期和凋亡
9 氧化应激作用
10 调节信号转导
11 骨替代材料的生物相容性评价方法
12 结语

图1 骨的结构[3]
Fig.1 Bone structure[3]

图2 骨替代材料在体内接触的细胞环境[12]<br/>Fig.2 In vivo cellular environment of bone substitute material[12]

图2 骨替代材料在体内接触的细胞环境[12]
Fig.2 In vivo cellular environment of bone substitute material[12]

图3 细胞形态异常[20](显示细胞结构紊乱,质膜破裂,内含物泄露)<br/>Fig.3 Abnormal cell morphology[20](featured by structural disorder, membrane rupture and contents leak)

图3 细胞形态异常[20](显示细胞结构紊乱,质膜破裂,内含物泄露)
Fig.3 Abnormal cell morphology[20](featured by structural disorder, membrane rupture and contents leak)

图4 HAP粒子进入细胞的TEM图[21]<br/>Fig.4 TEM image of HAP particles into cells[21]

图4 HAP粒子进入细胞的TEM图[21]
Fig.4 TEM image of HAP particles into cells[21]

图5 细胞黏附过程[25]<br/>Fig.5 Schematic of the cell adhesion process[25]

图5 细胞黏附过程[25]
Fig.5 Schematic of the cell adhesion process[25]

图6 线粒体介导的细胞凋亡途径[20]<br/>Fig.6 Endogenous apoptosis pathway mediated by mitochondria[20]

图6 线粒体介导的细胞凋亡途径[20]
Fig.6 Endogenous apoptosis pathway mediated by mitochondria[20]

图7 ROS介导材料颗粒产生细胞毒性的分子机制[32]<br/>Fig.7 Molecular mechanism of cytotoxicity produced by material particles mediated by ROS[32]

图7 ROS介导材料颗粒产生细胞毒性的分子机制[32]
Fig.7 Molecular mechanism of cytotoxicity produced by material particles mediated by ROS[32]

图8 RANKL和OPG的比例调节骨的重建[36]<br/>Fig.8 Bone reconstruction by regulating proportions of RANKL/OPG regulate[36]

图8 RANKL和OPG的比例调节骨的重建[36]
Fig.8 Bone reconstruction by regulating proportions of RANKL/OPG regulate[36]

图9 材料与生物体之间相互影响[38]<br/>Fig.9 Interaction between the bone substitute material and the organism[38]

图9 材料与生物体之间相互影响[38]
Fig.9 Interaction between the bone substitute material and the organism[38]

图10 生物材料的体外研究[40]<br/>Fig.10 In vitro studies of biological materials[40]

图10 生物材料的体外研究[40]
Fig.10 In vitro studies of biological materials[40]

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备注

引言

1 骨结构

2 骨替代及修复材料

3 骨替代高分子复合材料

4 骨替代材料在体内的细胞环境

5 细胞形态和结构与材料毒性的关系

6 骨替代材料的跨膜运输

7 细胞黏附与材料表面化学的关系

8 细胞增殖、周期和凋亡

9 氧化应激作用

10 调节信号转导

11 骨替代材料的生物相容性评价方法

12 结语

期刊信息

备注

引言