黑荆树提取物对铜绿微囊藻的化感抑制效应

四川大学建筑与环境学院,成都 610065

微生物学; 黑荆树提取物; 抑藻剂; 铜绿微囊藻; 化感作用; 抑制机理; 超微结构; 环境保护

Allelopathic inhibitory effect of the Acacia mearnsii extracts on Microcystis Aeruginosa
Luo Kai, Liu Dandan, Zhou Lirong, and Chen Wenqing

Luo Kai, Liu Dandan, Zhou Lirong, and Chen WenqingCollege of Architecture and Environment, Sichuan University, Chengdu 610065, P.R.China

microbiology; Acacia mearnsii extracts; algal inhibiting agent; Microcystis aeruginosa; allelopathy; inhibition mechanism; ultrastructure; environmental protection

DOI: 10.3724/SP.J.1249.2014.02205

备注

通过检测不同质量浓度黑荆树提取物处理下,不同处理时间段藻细胞浓度与叶绿素a含量的变化,结合细胞膜通透性检测及细胞微观结构,探究黑荆树提取物对藻细胞的抑制机理.研究发现,高浓度的黑荆树提取物降低了铜绿微囊藻细胞浓度和叶绿素a含量,增加了藻细胞膜通透性,藻细胞超微结构呈现明显损伤.黑荆树提取物作用于铜绿微囊藻细胞壁及细胞膜等微观结构,导致细胞膜通透性增加,细胞内物质流失; 在生理指标上表现为藻细胞生物量及叶绿素a含量的降低.

With the treatment of 0, 2, 10 and 20 mg/L algal inhibiting agents, Microcystis aeruginosa growth inhibition was measured by the density of algal cells, content of chlorophy-a, membrane permeability and ultrastructure of algal cell. It is found that 10 and 20 mg/L algal inhibiting agents reduce the concentration of algal cells and content of chlorophyll-a, increase the membrane permeability, and damage the ultrastructure of the algal cell. The Acacia mearnsii extracts increase the membrane permeability of Microcystis aeruginosa by damaging the ultrastructure of the algal cell, leading to the decrease of algal cells and chlorophyll-a.

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