[1]张毅哲,刘琼,田静,等.人脑中脱碘酶相互作用蛋白的筛选与验证[J].深圳大学学报理工版,2011,28(No.5(377-470)):423-429.
 ZHANG Yi-zhe,LIU Qiong,TIAN Jing,et al.Investigation on the interactive protein of iodothyronine deiodinase 3 mutant in human brain[J].Journal of Shenzhen University Science and Engineering,2011,28(No.5(377-470)):423-429.
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人脑中脱碘酶相互作用蛋白的筛选与验证()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第28卷
期数:
2011年No.5(377-470)
页码:
423-429
栏目:
生物工程
出版日期:
2011-09-20

文章信息/Info

Title:
Investigation on the interactive protein of iodothyronine deiodinase 3 mutant in human brain
文章编号:
1000-2618(2011)05-0423-07
作者:
张毅哲1刘琼1田静1邵永红2
1)深圳大学生命科学学院,深圳 518060
2)深圳大学光电工程学院,深圳 518060
Author(s):
ZHANG Yi-zhe1LIU Qiong1TIAN Jing1and SHAO Yong-hong2
1)College of Life Sciences, Shenzhen University, Shenzhen 518060, P.R.China
2)College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, P.R.China
关键词:
基因工程硒蛋白脱碘酶3大脑基因编码酵母双杂交系统蛋白质筛选荧光共振能量转移技术
Keywords:
genetic engineeringselenoproteiniodothyronine deiodinases 3(DIO3)braingene encodingyeast two-hybrid systemscreening of proteinfluorescence resonance energy transfer(FRET)
分类号:
Q 7;Q 5
文献标志码:
A
摘要:
阐述人脑内硒蛋白在维护细胞正常功能、抵御氧化损伤和预防脑疾病方面的重要作用,指出脱碘酶3是人脑中一种重要的硒蛋白.克隆了人脱碘酶3的开放读码框,将其编码区中编码硒代半胱氨酸的TGA码突变为编码半胱氨酸的密码,以脱碘酶3突变体为“诱饵”,利用酵母双杂交系统从人胎脑cDNA文库中筛选一个能与脱碘酶3相互作用的蛋白,即人丝氨酸蛋白酶抑制剂A族蛋白3.采用荧光共振能量转移技术中的敏化发射法和荧光寿命法,验证了人脑中脱碘酶的相互作用.
Abstract:
Selenoproteins play important roles in maintaining cell function,resisting peroxidation damage,and preventing brain diseases.Iodothyronine deiodinase 3 (DIO3) is an important selenoprotein in the human brain.In this study,a human DIO3 gene was cloned and mutated at the TGA codon in the open reading frame encoding for a selenocysteine (Sec) residue,where the Sec was site-mutated to a cysteine residue.The DIO3′ mutant was then used as a “bait” to screen a fetal brain cDNA library by yeast two-hybrid system.Preliminary investigation on the protein-protein interaction was performed by the life-time-resolved imaging measurement using the fluorescence resonance energy transfer technique.Results showed that the interactive protein of DIO3′ is a serpin peptidase inhibitor (clade A,member 3).

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备注/Memo

备注/Memo:
收稿日期:2010-08-16;修回日期:2011-06-23
基金项目:国家自然科学基金资助项目(31070731;30901182)
作者简介:张毅哲(1984-),男(汉族),江苏省无锡市人,深圳大学硕士研究生.E-mail:szunick2007@126.com
通讯作者:刘琼(1965-),女(汉族),深圳大学教授、博士生导师.E-mail:liuqiong@szu.edu.cn
更新日期/Last Update: 2011-09-23