[1]袁秋华,石鑫,吴文珊,等.羟基磷灰石-氧化石墨烯复合微球的制备及性能[J].深圳大学学报理工版,2022,39(4):447-455.[doi:10.3724/SP.J.1249.2022.04447]
 YUAN Qiuhua,SHI Xin,WU Wenshan,et al.Preparation and performance of hydroxyapatite-graphene oxide composite microspheres[J].Journal of Shenzhen University Science and Engineering,2022,39(4):447-455.[doi:10.3724/SP.J.1249.2022.04447]
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羟基磷灰石-氧化石墨烯复合微球的制备及性能()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第39卷
期数:
2022年第4期
页码:
447-455
栏目:
化学与化工
出版日期:
2022-07-12

文章信息/Info

Title:
Preparation and performance of hydroxyapatite-graphene oxide composite microspheres
文章编号:
202204012
作者:
袁秋华石鑫吴文珊代小毅钟骏熙杨袁简友亮李瑞龙王涛
深圳大学化学与环境工程学院,广东深圳 518071
Author(s):
YUAN Qiuhua SHI Xin WU Wenshan DAI Xiaoyi ZHONG Junxi YANG Yuan JIAN Youliang LI Ruilong and WANG Tao
College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518071, Guangdong Province, P. R. China
关键词:
生物医用材料复合微球羟基磷灰石氧化石墨烯模板法姜黄素载药性能
Keywords:
biomedical materials composite microspheres hydroxyapatite (HA) graphene oxide (GO) template method curcumin drug loading properties
分类号:
R318.08
DOI:
10.3724/SP.J.1249.2022.04447
文献标志码:
A
摘要:
载药微球目前在生物医用材料方面得到广泛研究,但存在载药量不高和突释等问题,为解决此类问题,利用羟基磷灰石(hydroxyapatite, HA)和氧化石墨烯(graphene oxide, GO)制备羟基磷灰石-氧化石墨烯(HA-GO)复合微球.首先采用硬模板法合成球状碳酸钙-氧化石墨烯(CaCO3-GO)复合材料,然后通过水热法以离子交换方式成功制备了球状中空HA-GO复合微球,研究不同合成条件对HA-GO复合材料的影响.通过X射线粉末衍射、傅里叶红外光谱、拉曼红外光谱、场发射扫描电子显微镜和紫外可见分光光度计等测试方法对所制备样品进行分析和表征,并以姜黄素作为载药模型对复合微球载药性能进行测试,通过包封率及载药量两个指标对复合微球的载药性能进行评估,同时测试微球样品材料的细胞毒性.研究表明:体系反应物的浓度及水热反应时间对复合微球的成型效果影响较大,在初始反应物浓度为0.3 mol/L、水热反应时间为6 h时,可制得形貌良好的中空HA-GO复合微球,所制的复合微球粒径为5.1~7.7 μm,孔径约为40 nm.研究还发现,球状结构能提高药物的负载量,复合微球的药物包封效率为(20.90 ± 0.31)%,载药量为(2.95 ± 0.19)%.由此表明,该方法制备的HA-GO复合微球有良好的医用价值.
Abstract:
Drug-loaded microspheres have been widely studied and applied in biomedical materials, but there are still some open problems such as low drug loading and sudden release. In order to solve such problems, hydroxyapatite-graphene oxide (HA-GO) composite microspheres were prepared by using hydroxyapatite (HA) and graphene oxide (GO). Firstly, spherical calcium carbonate-graphene oxide (CaCO3-GO) composites were synthesized by hard template method. Then, spherical hollow HA-GO composite microspheres were successfully prepared by a method of hydrothermal-assisted ion exchange. The effect of different synthesis conditions on the prepared HA-GO composites was studied. Through a series of measurements, such as X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Raman infrared spectroscopy, field emission scanning electron microscope (FESEM), ultraviolet visible spectrophotometer (UV-VIS), etc., the prepared samples were analyzed and characterized. The drug-loading performance of the microspheres was tested by using curcumin as the drug-loading model, and the drug-loading performance was evaluated by two indicators of encapsulation efficiency and drug-loading capacity. At the same time, the cytotoxicity test of the microsphere sample materials was also performed. The research results show that the reactant concentration of the system and the hydrothermal reaction time greatly affect the forming effect of the composite microspheres. When the initial reactant concentration is 0.3 mol/L and the hydrothermal reaction time is 6 h, hollow HA-GO composite microspheres with good morphology can be prepared, with a particle size from 5.1 μm to 7.7 μm and a pore size of about 40 nm. At the same time, it was found that the spherical structure can improve the drug loading, the drug encapsulation efficiency was (20.90 ± 0.31)%, and the drug loading was (2.95 ± 0.19)%. This shows that HA-GO composite microspheres have good medical application value.

参考文献/References:

[1] SUZUKI O, SHIWAKU Y, HAMAI R. Octacalcium phosphate bone substitute materials: comparison between properties of biomaterials and other calcium phosphate materials [J]. Dental Materials Journal, 2020, 39(2): 187-199.
[2] SOON Y M, SHIN K H, KOH Y H, et al. Fabrication and compressive strength of porous hydroxyapatite scaffolds with a functionally graded core/shell structure [J]. Journal of the European Ceramic Society, 2011, 31(1/2): 13-18.
[3] MESKINFAM M, BERTOLDI S, ALBANESE N, et al. Polyurethane foam/nano hydroxyapatite composite as a suitable scaffold for bone tissue regeneration [J]. Materials Science & Engineering C-Materials for Biological Applications, 2018, 82: 130-140.
[4] KATARZYNA S, AMANDA B, MARCIN P, et al. Electrochemical properties and bioactivity of hydroxyapatite coatings prepared by MEA/EDTA double-regulated hydrothermal synthesis [J]. Electrochimica Acta, 2019, 298: 685-693.
[5] WU Yanhong, CHEN Diyun KONG Lingjun, et al. Rapid and effective removal of uranium (VI) from aqueous solution by facile synthesized hierarchical hollow hydroxyapatite microspheres [J]. Journal of Hazardous Materials, 2019, 371: 397-405.
[6] NURETTIN S, COSKUN S, SELIN S, et al. Porous and modified HA particles as potential drug delivery systems [J]. Microporous and Mesoporous Materials, 2012, 155: 124-130.
[7] WU Yaping, CHEN Jing, CHEN Gang, et al. Convenient synthesis of hydroxyapatite-coated ferroferric oxide microspheres by hydrothermal method [J]. Materials Letters, 2019, 253: 218-221.
[8] AKINDOYO J O, BEG M D H, GHAZALI S, et al. Impact modified PLA-hydroxyapatite composites: thermomechanical properties [J]. Composites Part A: Applied Science and Manufacturing, 2018, 107: 326-333.
[9] DEVI G V Y, PRABHU A, ANIL S, et al. Preparation and characterization of dexamethasone loaded sodium alginate-graphene oxide microspheres for bone tissue engineering [J]. Journal of Drug Delivery Science and Technology, 2021, 64: 9.
[10] GAO Junguo, ZHAO He, SUN Weifeng. Molecular dynamics simulation study of parallel orientation structure and gas transport in graphite-nanoplatelet/polyethylene composites [J]. Materials Today Communications, 2017, 13: 57-64.
[11] MINA K, MOHAMMAD A H F, MAJID M, et al. Development of curcumin-loaded gemini surfactant nanoparticles: Synthesis, characterization and evaluation of anticancer activity against human breast cancer cell lines [J]. Phytomedicine, 2019, 57:183-190.
[12] TABANELLI R, BROGI S, CALDERONE V. Improving curcumin bioavailability: current strategies and future perspectives [J]. Pharmaceutics, 2021, 13(10): 37.
[13] MATLOUB Z, HASSAN Z. HSA-curcumin nanoparticles: a promising substitution for curcumin as a cancer chemoprevention and therapy [J]. Daru-Journal of Pharmaceutical Sciences, 2020, 28(1): 209-219.
[14] DIZAJ S M, ALIPOUR M, ABDOLAHINIA E D, et al. Curcumin nanoformulations: beneficial nanomedicine against cancer [J]. Phytotherapy Research, 2022, 36(3): 1156-1181.
[15] PRASADS, DUBOURDIEU D, SRIVASTAVA A, et al. Metal-curcumin complexes in therapeutics: an approach to enhance pharmacological effects of curcumin [J]. International Journal of Molecular Sciences, 2021, 22(13): 24.
[16] GUO Xia, LI Wenfeng, WANG Heping, et al. Preparation, characterization, release and antioxidant activity of curcumin-loaded amorphous calcium phosphate nanoparticles [J]. Journal of Non-Crystalline Solids, 2018, 500: 317-325.
[17] ZHENG Yaxin, LIU Xun, MA Yongjun, et al. Controlled synthesis of hydroxyapatite microspheres with hierarchical structure and high cell viability [J]. Materials Letters, 2017, 195: 18-21.
[18] TANUSHREE B, BONAMALI P, SATNAM S. Hollow chitosan nanocomposite as drug carrier system for controlled delivery of ramipril [J]. Chemical Physics Letters, 2018, 706: 465-471.
[19] 国家药典委员会.中华人民共和国药典[M]. 2005版2部. 北京:化学工业出版社,2005.
National Pharmacopoeia Commission. The Pharmacopoeia of the People’s Republic of China [M]. 2005 ed. Two. Beijing: Chemical Industry Press, 2005.(in Chinese)
[20] 马在强. 碳酸钙晶型调控及机理研究[D].大庆:东北石油大学,2019.
MA Zaiqiang. Regulation and mechanism of calcium carbonate crystal form [D]. Daqing: Northeast Petroleum University, 2019.(in Chinese)
[21] 袁秋华,陈泽汇,万磊,等. 锶掺杂羟基磷灰石-石墨烯复合材料制备与表征[J]. 深圳大学学报理工版, 2020,37(3):298-304.
YUAN Qiuhua, CHEN Zehui, WANG Lei, et al. Preparation and characterization of strontium-doped hydroxyapatite-graphene composites [J]. Journal of Shenzhen University Science and Engineering, 2020, 37(3): 298-304.(in Chinese)
[22] 袁秋华,石鑫,梁进仁,等. 铈锌共掺杂HA-GP复合物合成及抗菌性研究[J]. 深圳大学学报理工版, 2021,38(3):280-286.
YUAN Qiuhua, SHI Xing, LIANG Jinren, et al. Synthesis and antibacterial properties of Ce-Zn co-doped HA-GP complexes [J]. Journal of Shenzhen University Science and Engineering, 2021, 38(3): 280-286.(in Chinese)
[23] GUO Yajun, WANG Yingying, CHEN Ting, et al. Hollow carbonated hydroxyapatite microspheres with mesoporous structure: Hydrothermal fabrication and drug delivery property [J]. Materials Science and Engineering C, 2013, 33(6): 3166-3172.
[24] MILOVAC D, WEIGAND I, KOVACIC M, et al. Highly porous hydroxyapatite derived from cuttlefish bone as TiO2 catalyst support [J]. Processing and Application of Ceramics, 2018, 12(2): 136-142.
[25] El-MORSY M A, AWWAD N S, IBRAHIUM H A, et al. Optimizing the mechanical and surface topography of hydroxyapatite/Gd2O3/graphene oxide nanocomposites for medical applications [J]. Journal of Saudi Chemical Society, 2022, 26(3): 14.

备注/Memo

备注/Memo:
Received: 2022-04-23; Accepted: 2022-05-25; Online (CNKI): 2022-06-24
Foundation: Shenzhen Basic Research Foundation (JCYJ20190808110613626); National Natural Science Foundation of China (21471102)
Corresponding author: Associate professor YUAN Qiuhua. E-mail: yuanqiuh@szu.edu.cn
Citation: YUAN Qiuhua, SHI Xin, WU Wenshan, et al. Preparation and performance of hydroxyapatite-graphene oxide composite microspheres [J]. Journal of Shenzhen University Science and Engineering, 2022, 39(4): 447-455.(in Chinese)
基金项目:深圳市基础研究计划资助项目 (JCYJ20190808110613626);国家自然科学基金资助项目(21471102)
作者简介:袁秋华(1967—),深圳大学副教授、博士.研究方向:生物医用材料.E-mail: yuanqiuh@szu.edu.cn
引文:袁秋华,石鑫,吴文珊,等.羟基磷灰石-氧化石墨烯复合微球的制备及性能[J].深圳大学学报理工版,2022,39(4):447-455.
更新日期/Last Update: 2022-07-30