[1]陈必婷,吴炜城,周董董,等.两株海南红树植物内生真菌的生物活性成分[J].深圳大学学报理工版,2022,39(3):245-252.[doi:10.3724/SP.J.1249.2022.03245]
 CHEN Biting,WU Weicheng,ZHOU Dongdong,et al.Bioactive components of endophytic fungi from two Hainan mangrove plants[J].Journal of Shenzhen University Science and Engineering,2022,39(3):245-252.[doi:10.3724/SP.J.1249.2022.03245]
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两株海南红树植物内生真菌的生物活性成分()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第39卷
期数:
2022年第3期
页码:
245-252
栏目:
生命与健康
出版日期:
2022-05-16

文章信息/Info

Title:
Bioactive components of endophytic fungi from two Hainan mangrove plants
文章编号:
202203002
作者:
陈必婷12吴炜城2周董董1邓小林1张世清2袁靖喆2徐静1郭志凯23
1)海南大学化学工程与技术学院,海南海口570228
2)海南省热带微生物资源重点实验室,中国热带农业科学院热带生物技术研究所,海南海口571101
3)海南省热带农业生物资源保护与利用重点实验室,中国热带农业科学院海南热带农业资源研究院,海南海口571101
Author(s):
CHEN Biting12WU Weicheng2ZHOU Dongdong1DENG Xiaolin1ZHANG Shiqing2YUAN Jingzhe2 XU Jing1and GUO Zhikai23
1) School of Chemical Engineering and Technology, Hainan University, Haikou 570228, Hainan Province, P. R. China
2) Hainan Key Laboratory of Tropical Microbe Resources, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan Province, P. R. China
3) Hainan Key Laboratory of Conservation and Utilization of Tropical Agricultural Bioresources, Hainan Institute of Tropical Agricultural Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan Province, P. R. China
关键词:
海洋化学天然产物红树植物内生真菌次级代谢产物苯并呋喃酮脑苷脂类免疫抑制活性
Keywords:
marine chemistry natural products mangrove endophytic fungus secondary metabolites benzofuranone-type natural compound cerebroside-type natural product immunosuppressive activity
分类号:
Q939.92;R978.1
DOI:
10.3724/SP.J.1249.2022.03245
文献标志码:
A
摘要:
红树植物内生真菌是新型天然产物先导药物的重要来源之一.为获得具有新颖结构的化合物,对两株红树内生真菌Phomopsis sp. DHS11和Aspergillus fumigatus JRJ111048的代谢产物进行研究.综合利用正/反相硅胶柱、Sephadex LH20凝胶柱和HPLC高效液相色谱法,从发酵提取物中分离得到9种化合物,分别记为化合物1~9.利用高分辨电喷雾质谱(high resolution electrospray ionization mass spectroscopy, HRESIMS)、核磁共振波谱(nuclear magnetic resonance spectroscopy, NMR)、旋光、紫外和红外光谱等技术,鉴定化合物1~9依次为 norpestaphthalide C(1)、norpestaphthalide A(2-a)、norpestaphthalide B(2-b)、7-hydroxy-3-(2-hydroxy-propyl)-5-methyl-isochromen-1-one (3)、alternaroside B (4)、细胞松驰素B (5)、啤酒甾醇(6)、5-羟甲基糠醛(7)、N-(4-羟基苯乙基)-乙酰胺(8)和3,5-dihydroxybenzoic acid (9).其中,化合物1为首次发现的新型苯并呋喃酮类化合物,化合物4是首次从Phomopsis sp.属真菌中分离得到的脑苷脂类化合物.对化合物1~9进行体外免疫抑制、抗肿瘤和抗老年痴呆活性评价,结果表明,细胞松弛素类化合物5具有良好的免疫抑制活性.研究结果为深入挖掘红树植物内生真菌新型生物活性物质提供了参考.
Abstract:
Mangrove endophytic fungi are one of the important sources for discovering natural drug leads. In order to obtain natural compounds with novel structures, the metabolites of two mangrove endophytic fungi Phomopsis sp. DHS11 and Aspergillus fumigatus JRJ111048 were studied. As a result, nine compounds (1-9) were isolated from their fermentation extract by comprehensive silica gel, reverse phase silica gel, Sephadex LH20 column chromatography and high performance liquid chromatography (HPLC). The structures of these compounds were identified as norpestaphthalide C (1), norpestaphthalide A (2-a), norpestaphthalide B (2-b), 7-hydroxy-3-(2-hydroxy-propyl)-5-methyl-isochromen-1-one (3), alternaroside B (4), cytochalasin B (5), cerevisterol (6), 5-hydroxymethyl-furaldehyde (7), N-(4-hydroxyphenethyl) acetamide (8), 3,5-dihydroxybenzoic acid (9) by using high resolution electrospray mass spectrometry (HRESIMS), nuclear magnetic resonance spectroscopy (NMR), optical rotation, ultraviolet, infrared spectroscopy techniques and comparing with reported data. Among them, compound 1 was a new benzofuranone-type natural compound discovered for the first time, and the compound 4 was a cerebroside-type natural product reported for the first time from Phomopsis sp. The immunosuppressive activity, antitumor activity and anti-Alzheimer’s activity of compounds 1-9 were evaluated. The result showed that compound 5 displayed good immunosuppressive activity. The research results provide new clues for the in-depth exploration of new bioactive substances in mangrove endophytes.

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备注/Memo

备注/Memo:
Received: 2021-10-05; Accepted: 2021-10-26; Online (CNKI): 2022-04-06
Foundation: The Central Public-Interest Scientific Institution Basal Research Fund for CATAS-ITBB (1630052019011); Key Research Program of Hainan Province (ZDYF2021108)
Corresponding author: Associate professor GUO Zhikai. E-mail: guozhikai@itbb.org.cn
Professor XU Jing. E-mail: happyjing3@163.com
Citation: CHEN Biting, WU Weicheng, ZHOU Dongdong, et al. Bioactive components of endophytic fungi from two Hainan mangrove plants [J]. Journal of Shenzhen University Science and Engineering, 2022, 39(3): 245-252.(in Chinese)
基金项目:中央公益性科研院所基本科研业务资助项目(1630052019011)海南省重点研发计划资助项目(ZDYF2021108);海南省重大科技计划资助项目(ZDKJ202008,ZDKJ202018);国家自然科学基金资助项目(81973229,82160675)
作者简介:陈必婷(1995—),海南大学硕士研究生.研究方向:微生物天然产物化学.E-mail: cbt688228@163.com
引文:引用格式:陈必婷,吴炜城,周董董,等.两株海南红树植物内生真菌的生物活性成分[J].深圳大学学报理工版,2022,39(3):245-252.
更新日期/Last Update: 2022-05-30