[1]李笑玥,秦华鹏,王凡,等.生物滞留池微生物种群的硝化反硝化功能研究——以深圳市为例[J].深圳大学学报理工版,2021,38(1):36-44.[doi:10.3724/SP.J.1249.2021.01036]
　LI Xiaoyue,QIN Huapeng,WANG Fan,et al.Nitrification and denitrification functions of microbial population in bioretention cells: a case study of Shenzhen[J].Journal of Shenzhen University Science and Engineering,2021,38(1):36-44.[doi:10.3724/SP.J.1249.2021.01036]
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生物滞留池微生物种群的硝化反硝化功能研究
——以深圳市为例()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第38卷
期数:
2021年第1期
页码:
36-44
栏目:
专题：海绵城市
出版日期:
2021-01-12

文章信息/Info

Title:
Nitrification and denitrification functions of microbial population in bioretention cells: a case study of Shenzhen
文章编号:
202101005
作者:
李笑玥1; 秦华鹏1; 王凡2; 银翼翔1
1）北京大学深圳研究生院环境与能源学院，城市人居环境科学与技术重点实验室，广东深圳 518055
2）中山大学大气科学学院，广东珠海519082
Author(s):
LI Xiaoyue1;  QIN Huapeng1;  WANG Fan2;  and YIN Yixiang1
1) School of Environment and Energy, Peking University Shenzhen Graduate School, Key Laboratory for Urban Habitat Environmental Science and Technology, Shenzhen 518055, Guangdong Province, P.R.China
2) School of Atmospheric Sciences, Sun Yat-sen University, Zhuhai 519082, Guangdong Province, P.R.China
关键词:
海绵城市; 环境科学; 生物滞留池; 高通量测序; 硝化; 反硝化; 微生物群落结构; 功能基因
Keywords:
sponge city;  environmental science;  bioretention cell;  high-throughput sequencing;  nitrification;  denitrification;  microbial community structure;  functional gene
分类号:
X52
DOI:
10.3724/SP.J.1249.2021.01036
文献标志码:
A
摘要:
生物滞留池是海绵城市建设中净化城市径流的重要设施，其中的微生物在径流氮处理中起到关键作用．为揭示生物滞留池氮去除机理，利用16S核糖体核糖核酸（ribosomal ribonucleic acid, rRNA）基因-Illumina MiSeq 高通量测序技术和实时荧光定量的方法，调查中国深圳市8个典型生物滞留池中总细菌的多样性与菌门水平分布，分析其中硝化与反硝化功能菌属的群落结构、功能基因丰度及其与环境因子的关系．结果表明：① 生物滞留池的主要优势菌门为变形菌门、放线细菌门、拟杆菌门、酸杆菌门、绿弯菌门和厚壁菌门，这些优势菌门的相对丰度占群落的93.49%~94.80%．② 微生物群落中相对丰度最高的硝化功能菌是亚硝化单胞菌科和硝化螺旋菌属；相对丰度最高的反硝化功能菌是芽孢杆菌属，其次是赖氨酸芽孢杆菌属和假单胞菌属．③ 在不同生物滞留池中硝化和反硝化功能基因拷贝数表现出较大差异，其中，市政道路旁的生物滞留池采样点的硝化和反硝化功能基因丰度均呈现较高值．④ 生物滞留池中氮转化微生物的多样性及功能基因的丰度受体系结构、植被类型与污染负荷的综合作用，反硝化菌更容易受到环境因子的影响．本研究的微生物调查结果可为生物滞留池的氮去除功能的评估和提高提供思路．
Abstract:
Bioretention cells are important facilities for purification of urban runoff in the sponge city construction, and the inside microorganisms play a key role in the treatment of runoff nitrogen. In order to reveal the mechanism of nitrogen removal in bioretention cells, 16S ribosomal ribonucleic acid (rRNA) gene-Illumina MiSeq high-throughput sequencing technology and real-time fluorescence quantification method are used to investigate the diversity and phylum-level distribution of total bacteria in 8 typical bioretention cells in Shenzhen, and analyze the community structure, functional gene abundance of nitrifying and denitrifying functional microorganisms and their relationship with environmental factors. The results show that: ① The main predominant phyla in the bioretention cells are Proteobacteria, Actinomycetes, Bacteroides, Acidobacteria,Chloroflexi, and Firmicutes. Their relative abundance accounts for 93.49%~94.80% of the community. ② The most abundant nitrifying bacteria in the microbial community are Nitrosomonadaceae and Nitrospira; the most abundant denitrifying bacteria are Bacillus, followed by Lysinibacillus and Pseudomonas. ③ The copy numbers of nitrification and denitrification functional genes in different bioretention cells show large differences, and the abundance of nitrification and denitrification functional genes in the samples of bioretention cells beside municipal roads all show higher values. ④ The diversity of nitrogen-converting microorganisms and the abundance of functional genes in bioretention cells are affected by the combined effects of system structure, vegetation type, and pollution load. Denitrifying bacteria are more susceptible to environmental factors.This study can provide ideas for the evaluation and improvement of the nitrogen removal function of bioretention cells.

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备注/Memo

备注/Memo:
Received：2020-04-26；Accepted：2020-06-18
Foundation：Shenzhen Basic Research Foundation（JCYJ20170818090113750）; National Natural Science Foundation for Young Scholars of China（41603073）
Corresponding author：Professor QIN Huapeng. E-mail: qinhp@pku.edu.cn
Citation：LI Xiaoyue, QIN Huapeng, WANG Fan, et al. Nitrification and denitrification functions of microbial population in bioretention cells: a case study of Shenzhen［J］. Journal of Shenzhen University Science and Engineering, 2021, 38(1): 36-44.（in Chinese）
基金项目：深圳市基础研究计划资助项目（JCYJ201708180901137 50）；国家自然科学基金青年基金资助项目（41603073）
作者简介：李笑玥（1994—），北京大学硕士研究生. 研究方向：海绵城市建设的生态理论与方法．E-mail：1701213693@pku.edu.cn
通讯作者：秦华鹏（1973—），北京大学教授、博士生导师.研究方向：城市水环境与水资源. E-mail：qinhp@pku.edu.cn
引文：李笑玥，秦华鹏，王凡，等. 生物滞留池微生物种群的硝化反硝化功能研究——以深圳市为例［J］. 深圳大学学报理工版，2021，38(1)：36-44.
更新日期/Last Update: 2021-01-26