[1]罗顺,刘冰,张金龙,等.混凝土自修复菌Bacillus sp.芽孢生产和萌发条件的优化[J].深圳大学学报理工版,2020,37(1):25-32.[doi:10.3724/SP.J.1249.2020.01025]
 LUO Shun,LIU Bing,ZHANG Jinlong,et al.Optimization of sporulation and germination of self-healing bacterium Bacillus sp. for concrete crack[J].Journal of Shenzhen University Science and Engineering,2020,37(1):25-32.[doi:10.3724/SP.J.1249.2020.01025]
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混凝土自修复菌Bacillus sp.芽孢生产和萌发条件的优化()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第37卷
期数:
2020年第1期
页码:
25-32
栏目:
生物工程
出版日期:
2020-01-08

文章信息/Info

Title:
Optimization of sporulation and germination of self-healing bacterium Bacillus sp. for concrete crack
文章编号:
202001004
作者:
罗顺1刘冰2张金龙1李秋伟1邓旭1
1)深圳大学生命与海洋科学学院,广东深圳 518055
2)深圳信息职业技术学院交通与环境学院,广东深圳 518172
Author(s):
LUO Shun1 LIU Bing2 ZHANG Jinlong1 LI Qiuwei1 and DENG Xu1
1) College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518055, Guangdong Province, P.R.China
2) School of Traffic and Environment, Shenzhen Institute of Information Technology, Shenzhen 518172, Guangdong Province, P.R.China
关键词:
微生物学自修复枯草芽孢菌属芽孢生成萌发矿化
Keywords:
microbiology self-healing Bacillus sporulation germination mineralization
分类号:
Q819;Q939.99
DOI:
10.3724/SP.J.1249.2020.01025
文献标志码:
A
摘要:
对一株混凝土自修复菌Bacillus sp.B8芽孢生产条件进行优化,研究芽孢萌发的影响因素.芽孢生产的单因素实验表明,最适接种量为1×107 mL-1;最适碳源和氮源分别为淀粉和酵母粉,相应的最适质量浓度分别为0.5 g/L和2 g/L;一定质量浓度的Mg2+(0.24 g/L)和Mn2+(4.8 mg/L)有利于芽孢生成. 在单因素实验的基础上利用正交实验对各影响因子进行了优化,结果表明,接种量和酵母粉浓度是影响芽孢产量的显著因子,在接种量、淀粉、酵母粉、Mg2+和Mn2+浓度分别为1×107 mL-1、5、5、0.48和4.8×10-3 g/L时,芽孢产量达到1.54×109 mL-1,比优化前提高150倍. 此外,芽孢萌发实验表明,芽孢萌发时间约为4 h;最适萌发剂为肌苷,最适质量浓度为2 g/L,最适pH值为10;金属离子Na+能促进芽孢萌发,但Ca2+对芽孢的萌发有严重的抑制作用.在实验中首次发现一定质量浓度的Na+能消除Ca2+的抑制作用,最适质量浓度为24 g/L.
Abstract:
The single factor experimental results of spore production of a self-healing bacterial strain, Bacillus sp.B8, show that the optimal inoculation volume is 1×107 mL-1. The suitable carbon and nitrogen source are starch and yeast extract and the corresponding optimal concentrations being 0.5 g/L and 2 g/L, respectively. The presence of Mg2+ (0.24 g/L) and Mn2+ (4.8 mg/L) is beneficial for spore formation. Furthermore, based on the above results, orthogonal experiment is carried out to optimize the influential factors. The result shows that the inoculation volume and yeast extract concentration are the significant factors affecting spore yield. Under the optimized condition in which the concentrations of inoculation cells, starch, yeast extract, Mg2+ and Mn2+ are 1×107 mL-1, 5, 5, 0.48 and 4.8×10-3 g/L, respectively, the final spore concentration reaches up to 1.54×109 mL-1, showing more than 150 times enhancement. On the other hand, the results of spore germination indicate that spores need about 4 h to complete germination process. The most suitable germinant is inosine, and the optimal concentration is 2 g/L. The suitable pH is 10. Na+ could promote the germination of spores, whereas Ca2+ shows a severe inhibition on the germination of spores. However, we find, for the first time, that the presence of Na+ at a certain concentration (the optimal concentration is 24 g/L) can alleviate the inhibition of Ca2+ on spore germination to a large extent.

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备注/Memo

备注/Memo:
Received:2019-05-20;Accepted:2019-09-19
Foundation:National Natural Science Foundation of China (51578339); Project of Department of Education of Guangdong Province (2017GKTSCX064)
Corresponding author:Professor DENG Xu. E-mail: dengxu@szu.edu.cn
Citation:LUO Shun,LIU Bing,ZHANG Jinlong,et al. Optimization of sporulation and germination of self-healing bacterium Bacillus sp. for concrete crack[J]. Journal of Shenzhen University Science and Engineering, 2020, 37(1): 25-32.(in Chinese)
基金项目:国家自然科学基金资助项目(51578339);广东省高等学校科技创新资助项目(2017GKTSCX064)
作者简介:罗顺(1995—),男,深圳大学硕士研究生.研究方向:混凝土的微生物自修复. E-mail:luoshun2017@email.szu.edu.cn
引文:罗顺,刘冰,张金龙,等. 混凝土自修复菌Bacillus sp.芽孢生产和萌发条件的优化[J]. 深圳大学学报理工版,2020,37(1):25-32.
更新日期/Last Update: 2020-01-30