[1]钟姗,等.食管鳞癌中3个新miRNA的分子功能预测[J].深圳大学学报理工版,2019,36(4):347-353.[doi:10.3724/SP.J.1249.2019.04347]
　ZHONG Shan,WANG Yun,et al.The prediction of molecular functions for three novel miRNAs in esophageal squamous cell carcinoma[J].Journal of Shenzhen University Science and Engineering,2019,36(4):347-353.[doi:10.3724/SP.J.1249.2019.04347]
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食管鳞癌中3个新miRNA的分子功能预测()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第36卷
期数:
2019年第4期
页码:
347-353
栏目:
【生物工程】
出版日期:
2019-07-10

文章信息/Info

Title:
The prediction of molecular functions for three novel miRNAs in esophageal squamous cell carcinoma
文章编号:
201904001
作者:
钟姗1;  2; 王筠1; 刘乃嘉1; 颜鸿飞3; 李延鹏1; 张庆英2; 盛司潼1
1）深圳大学生命与海洋科学学院，广东深圳518055
2）汕头大学医学院预防医学教研室，广东汕头515041
3）汕头大学医学院附属肿瘤医院，广东汕头515000
Author(s):
ZHONG Shan1;  2;  WANG Yun1;  LIU Naijia1;  YAN Hongfei3;  LI Yanpeng1;  ZHANG Qingying2;  and SHENG Sitong1
1) College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518055, Guangdong Province, P.R.China
2) Department of Preventive Medicine, Shantou University Medical College, Shantou 515041, Guangdong Province, P.R.China
3) Pathology Laboratory, Cancer Hospital, Shantou University Medical College, Shantou 515000, Guangdong Province, P.R.China
关键词:
病理生理学; 食管鳞癌; 微小RNA; 生物信息学; 癌症相关通路; 生物标记物
Keywords:
pathophysiology;  esophageal squamous cell carcinoma;  miRNA;  bioinformatics;  pathway in cancer;  biomarkers
分类号:
R363.1+4；R735.1
DOI:
10.3724/SP.J.1249.2019.04347
文献标志码:
A
摘要:
为寻找食管鳞癌（esophageal squamous cell carcinoma, ESCC）诊断与治疗的新分子标志物，利用实时定量荧光PCR技术检测ESCC中3个未知miRNAs（miR-3914、miR-8082和miR-4770）的表达状况；运用生物信息学方法分别对它们差异表达miRNA下游的候选靶基因做基因本体（gene ontology, GO）富集和KEGG(Kyoto encyclopedia of genes and genomes)富集分析；采用蛋白免疫印迹方法观察转染miRNA mimic后一些与癌症发生和发展相关蛋白的表达改变．结果显示，与食管癌癌旁正常组织和正常食管上皮细胞比较，在ESCC组织/细胞中的miR-3914和miR-8082表达均呈显著性降低（P<0.05）， 但miR-4770无明显的表达变化．GO富集分析发现，miR-3914和miR-8082参与RNA聚合酶II启动子转录、蛋白结合和细胞凋亡等生物学过程；KEGG富集分析发现，miR-3914和miR-8082两者均与癌症相关通路有关, 且均可调控LAMC2、 CCDC6和PDGFB基因．当ESCC细胞分别转染miR-3914 mimic和miR-8082 mimic后，与未转染组比较，p53蛋白质表达增加，而c-Myc和Bcl-2蛋白质表达降低．研究结果表明，miR-3914和miR-8082各自均具有成为辅助ESCC早期诊断与精准治疗生物靶标的潜能．
Abstract:
In order to find novel biomarkers for the diagnosis and treatment of esophageal squamous cell carcinoma (ESCC), qRT-PCR was used to detect the expression of three unknown miRNAs (miR-3914, miR-8082 and miR-4770) in ESCC. GO (gene ontology) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analyses were performed to evaluate and predict biological functions of targeted genes in differentially expressed miRNAs. Westernblot was used to analyzecancer-related protein expression after miRNA mimic transfection. Results showed that miR-3914 and miR-8082 were significantly decreased in ESCC tissues/cells compared with normal esophageal tissues/cells (P<0.05). Data of GO enrichment analysis revealed that miR-3914 and miR-8082 could participate in RNA polymerase II promoter transcription, protein binding, cell apoptosis and other biological processes. KEGG pathway analysis displayed that both miR-3914 and miR-8082 were associated with pathway in cancer, simultaneously regulated to LAMC2, CCDC6 and PDGFB genes. After transfection of miR-3914 and miR-8082 mimic, the expression of p53 protein was increased and accompanied with the decreases of c-Myc and Bcl-2proteins in ESCC cells compared to untreated cells. Taken together, both miR-3914 and miR-8082 may become novel potential biomarkers for helping early diagnosis and precision treatment of ESCC.

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备注/Memo

备注/Memo:
Received：2018-10-15；Accepted：2019-03-02
Foundation：National High-tech R & D Program（2014AA022402); Shenzhen Science and Technology Basic Research Foundation (JCYJ20160520174247964)
Corresponding author：Professor WANG Yun. E-mail: yunw@szu.edu.cn
Citation：ZHONG Shan, WANG Yun, LIU Naijia, et al. The prediction of molecular functions for three novel miRNAs in esophageal squamous cell carcinoma［J］. Journal of Shenzhen University Science and Engineering, 2019, 36(4): 347-353.（in Chinese）
基金项目：国家高技术研究发展计划资助项目（2014AA022402）；深圳市科技基础研究计划资助项目（JCYJ20160520 174247964）
作者简介：钟姗（1990—），汕头大学博士研究生．研究方向：病理生理学．E-mail：shanzhong33@126.com
引文：钟姗，王筠，刘乃嘉，等．食管鳞癌中3个新miRNA的分子功能预测［J］. 深圳大学学报理工版，2019，36(4)：347-353.
更新日期/Last Update: 2019-07-04