Deng Xu,Huang Huiwei,Liang Cailiu,et al.Analysis of a marine bacterium with high mercury resistance and bioaccumulation capacity[J].Journal of Shenzhen University Science and Engineering,2016,33(No.6(551-660)):551-557.[doi:10.3724/SP.J.1249.2016.06551]





Analysis of a marine bacterium with high mercury resistance and bioaccumulation capacity
Deng Xu Huang Huiwei Liang Cailiu and Liu Bing
College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China
genetic engineering Pseudomonas transcriptomics mercury resistance gene genetically engineered bacterium bioaccumulation
X 55; Q 789
为探究一株海洋类产碱假单胞菌A1高汞抗性和高富集能力的机制,从分子水平出发,对汞胁迫(或无汞胁迫)条件下的A1进行转录组学分析,得到差异表达基因427个.从中筛选出差异表达倍数较大且与重金属抗性相关的基因Unigene97-All, 经Blast分析和构建系统进化树,并构建基因工程菌进行抗性和生物富集性能验证.结果表明, Unigene97-All为MerA汞还原酶编码基因,其编码的氨基酸序列与同样来自假单胞菌的MerA (KJU79639.1)序列具有90%的相似性.构建成功的重组菌U97具有一定的抗汞能力,在Hg2+质量浓度为6.0 mg/L的培养基中能正常生长,但重组菌U97和对照组大肠杆菌的汞富集量无明显差异,说明细菌的汞富集量与抗性没有必然的联系.
A transcriptome analysis of an ocean Pseudomonas pseudoalcaligenes A1 with/without Hg2+ stress was carried out at the molecular level in order to explore the mechanism of high mercury resistance and bioaccumulation capacity. The number of differentially expressed genes induced by Hg2+ is 427. Unigene97-All was found to be a greatly-differentially expressed gene correlating with the resistance to Hg2+ by Blast analysis and phylogenetic tree construction. Then a recombinant bacterium was constructed to evaluate the role of Unigene97-All gene in mercury resistance and bioaccumulation capacity. The results suggest that the Unigene97-All is a mercury reductase MerA gene, and its coding sequence has a 90% similarity to that of the Pseudomonas MerA (KJU79639.1). The recombinant bacterium is capable of growing in the presence of 6.0 mg/L Hg2+, higher than the 2.0 mg/L for the control bacterium, but the maximum accumulation capacity of the recombinant cells shows no difference compared with that of the control. Results indicate that no direct connection between the heavy metal resistance and the metal bioaccumulation capacity of the bacteria exists.


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Foundation:National Natural Science Foundation of China (51578339); Shenzhen Science and Technology Research Foundation(JCYJ20140418193546101)
Corresponding author:Professor Deng Xu, E-mail: dengxu@szu.edu.cn
Citation:Deng Xu, Huang Huiwei, Liang Cailiu, et al. Analysis of a marine bacterium with high mercury resistance and bioaccumulation capacity [J]. Journal of Shenzhen University Science and Engineering, 2016, 33(6): 551-557.(in Chinese)
作者简介:邓旭(1968— ),男,深圳大学教授.研究方向:环境生物技术.E-mail: dengxu@ezu.edu.cn
引文:邓旭,黄慧炜,梁彩柳,等.一株海洋细菌高汞抗性及高富集能力的分析[J]. 深圳大学学报理工版,2016,33(6):551-557.
更新日期/Last Update: 2016-10-31