Ma Mingming,Zhang Yan,and Zhao Qiang.Spectroscopy of interaction of herring fine dsDNA and benzidine[J].Journal of Shenzhen University Science and Engineering,2016,33(5):457-463.[doi:10.3724/SP.J.1249.2016.05457]





Spectroscopy of interaction of herring fine dsDNA and benzidine
西安工程大学环境与化学工程学院,陕西西安 710048
Ma Mingming Zhang Yan and Zhao Qiang
College of Environment and Chemical Engineering, Xi’an Polytechnic University, Xi’an 710048, Shaanxi Province, P.R.China
分光光度法荧光光谱紫外可见吸收光谱联苯胺鲱鱼精双链脱氧核糖核酸嵌插 脱氧核糖核酸熔点
spectrophotometry fluorescence spectroscopy ultraviolet visible benzidine herring fine double-stranded deoxyribonucleic acid (dsDNA) intercalation DNA melting point
O 657.3
在pH值为 7.38的磷酸盐缓冲溶液(phosphate buffer saline,PBS)中,采用荧光光谱和紫外-可见吸收光谱,考察联苯胺(benzidine,BZ)与鲱鱼精双链脱氧核糖核酸(double-stranded deoxyribonucleic acid, dsDNA)的相互作用机制,运用离子强度验证结合体系(BZ-dsDNA)荧光光谱的影响和DNA熔点变化两种方法的结合模式.结果表明,BZ以嵌插方式与鲱鱼精dsDNA结合,结合作用力主要是氢键和范德华力,结合反应为自发过程.实验还发现,当dsDNA质量浓度为8.00×10-6~48.00×10-6 g/L时,BZ荧光强度F0和结合体系BZ-dsDNA荧光强度F的比值(F0/F )与dsDNA质量浓度呈线性关系,dsDNA的检测限为2.00×10-6 g/L,且其测定不受常见共存金属离子和抗坏血酸等的干扰.因此,基于BZ与dsDNA作用的荧光光谱建立的检测dsDNA的新方法,可用于检测实验室废水中的dsDNA,平均回收率在98.00%~103.00%.
The interaction mechanism of benzidine (BZ) with herring fine dsDNA in PBS (pH=7.38) is investigated by the fluorescence spectroscopy and the UV-visible absorption spectroscopy. Their binding mode is verified by two methods. One examines the effect of the ionic strength on the fluorescence intensity of the binding system BZ-dsDNA. The other is an DNA melting experiment. The results show that BZ combines with dsDNA in the mode of intercalation mainly by hydrogen bonding and van der Waals forces, and the interaction reaction is spontaneous. In addition, the ratio of fluorescence intensity of BZ (F0) to BZ-dsDNA (F) is linearly proportional to the concentration of dsDNA in the range of 8.00×10-6–48.00×10-6 g/L with a detection limit of 2.00×10-6 g/L. The common co-existed metal ions and ascorbic acid do not interfere with the determination. A new fluorescence spectroscopy method for the determination of dsDNA is developed based on the interaction between dsDNA and BZ. This method is applied into determining dsDNA in waste water from the lab which achieves the average recovery between 98.00% and 103.00%.


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Foundation:Program for Science & Technology of Shaanxi Province (2013kj07-24)
Corresponding author:Professor Ma Mingming. E-mail: 18220593193@163.com
Citation:Ma Mingming, Zhang Yan, Zhao Qiang. Spectroscopy of interaction of herring fine dsDNA and benzidine[J]. Journal of Shenzhen University Science and Engineering, 2016, 33(5): 457-463.(in Chinese)
作者简介:马明明(1969—),女,西安工程大学教授. 研究方向:功能材料制备与应用.E-mail:18220593193@163.com
引文:马明明,张彦,赵强.鲱鱼精dsDNA和联苯胺相互作用的光谱分析[J]. 深圳大学学报理工版,2016,33(5):457-463.
更新日期/Last Update: 2016-09-08