[1]胡章立,李建成,程雪薇.莱茵衣藻线粒体遗传系统及其外源基因表达[J].深圳大学学报理工版,2013,30(No.6(551-660)):603-610.[doi:10.3724/SP.J.1249.2013.06603]
 Hu Zhangli,Li Jiancheng,and Cheng Xuewei.The mitochondrial genetic system and exogenous gene expression of Chlamydomonas reinhardtii[J].Journal of Shenzhen University Science and Engineering,2013,30(No.6(551-660)):603-610.[doi:10.3724/SP.J.1249.2013.06603]
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莱茵衣藻线粒体遗传系统及其外源基因表达()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第30卷
期数:
2013年No.6(551-660)
页码:
603-610
栏目:
生物工程
出版日期:
2013-09-30

文章信息/Info

Title:
The mitochondrial genetic system and exogenous gene expression of Chlamydomonas reinhardtii
文章编号:
20130609
作者:
胡章立李建成程雪薇
深圳市海洋生物资源与生态环境重点实验室,深圳市海洋藻类开发应用工程实验室,深圳大学生命科学学院,深圳 518060
Author(s):
Hu Zhangli Li Jiancheng and Cheng Xuewei
Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Shenzhen Engineering Laboratory of Marine Algal Biotechnology, College of Life Science, Shenzhen University, Shenzhen 518060, P.R.China
关键词:
基因工程遗传转化莱茵衣藻线粒体基因组外源基因表达线粒体遗传基因调控
Keywords:
genetic engineering genetic transformation Chlamydomonas reinhardtii mitochondrial genome exogenous gene expression mitochodrial genetics expression regulation
分类号:
Q 785
DOI:
10.3724/SP.J.1249.2013.06603
文献标志码:
A
摘要:
外源基因表达是目前光合生物线粒体遗传系统研究的瓶颈.作者在成功进行光合生物线粒体外源基因稳定表达的基础上,评述光合生物——莱茵衣藻的线粒体遗传表达系统,指出该系统是目前唯一能够有效表达外源功能基因的光合生物.介绍莱茵衣藻线粒体基因组结构、mtDNA复制、转录及翻译特性,论述莱茵衣藻线粒体遗传转化方法、转化株筛选标记基因及外源基因表达的研究进展,分析外源基因在线粒体基因组中的同质化过程、表达效率及存在问题,为莱茵衣藻线粒体基因表达调控及反向遗传学研究辟出新径.
Abstract:
The efficient expression of an exogenous gene in mitochondria of photosynthetic organism has been an insurmountable problem. The authors carried out the first successful stable expression of foreign egfp in the mitochondria of Chlamydomonas reinhardtii. Based on these results, this review described briefly mitochondrial genetic expression system of Chlamydomonas reinhardtii which indicating that it is the only photosynthetic organism that can utilize the mitochondrial genetic system to express exogenous genes effectively up to date. Meanwhile, mitochondrial genome structure, the replication of mtDNA, the transcription and translation is introduced. The latest research progresses are reviewed for the mitochondrial transformation method, screening markers and expression levels of exogenous genes Chlamydomonas reinhardtii. In addition, the homogenization processes of an exogenous gene in the mitochondrial genome and the expression efficiency of as well as the influence factors were analysed. Further development of mitochondrial gene expression and reverse genetics were also discussed in Chlamydomonas reinhardtii.

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备注/Memo

备注/Memo:
Received:2013-09-10;Accepted:2013-11-07
Foundation:National Natural Science Foundation of China (31070323, 41176106)
Corresponding author:Professor Hu Zhangli. E-mail: huzl@szu.edu.cn
Citation:Hu Zhangli, Li Jiancheng, Cheng Xuewei. The mitochondrial genetic system and exogenous gene expression of Chlamydomonas reinhardtii[J]. Journal of Shenzhen University Science and Engineering, 2013, 30(6): 603-610.(in Chinese)
基金项目:国家自然科学基金资助项目(31070323, 41176106)
作者简介:胡章立(1964-),男(汉族),湖南省常德市人,深圳大学教授、博士生导师. E-mail: huzl@szu.edu.cn
引文:胡章立,李建成,程雪薇. 莱茵衣藻线粒体遗传系统及其外源基因表达[J]. 深圳大学学报理工版,2013,30(6):603-610.
更新日期/Last Update: 2013-11-20