[1]倪卓,刘士德,王应,等.PEEK-HA生物材料对成纤维细胞3T3的影响[J].深圳大学学报理工版,2013,30(No.4(331-440)):392-397.[doi:10.3724/SP.J.1249.2013.04392]
　Ni Zhuo,Liu Shide,Wang Ying,et al.Influence of 3T3 cells for PEEK-HA composite[J].Journal of Shenzhen University Science and Engineering,2013,30(No.4(331-440)):392-397.[doi:10.3724/SP.J.1249.2013.04392]
点击复制

PEEK-HA生物材料对成纤维细胞3T3的影响()
分享到：

《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第30卷
期数:
2013年No.4(331-440)
页码:
392-397
栏目:
材料科学
出版日期:
2013-07-12

文章信息/Info

Title:
Influence of 3T3 cells for PEEK-HA composite
文章编号:
20130408
作者:
倪卓1; 刘士德2; 王应2; 刘学3; 姜振华4
1) 深圳大学化学与化工学院，深圳 518060
2) 深圳大学生命科学学院，深圳 518060
3) 深圳市第六人民医院，深圳 518067
4）吉林大学化学系，长春 130012
Author(s):
Ni Zhuo1;  Liu Shide2;  Wang Ying2;  Liu Xue3;  and Jiang Zhenhua4
1) College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen 518060, P.R.China
2) College of Life Science, Shenzhen University, Shenzhen 518060, P.R.China
3) Shenzhen Sixth People’s Hospital, Shenzhen 518067, P.R.China
4) Department of Chemistry, Jilin University, Changchun 130012, P.R.China
关键词:
复合材料; 骨替代材料; PEEK-HA生物复合材料; 生物相容性; 3T3细胞; 细胞增殖; 细胞毒性; 黏附率
Keywords:
composite material;  bone substitute materials;  PEEK-HA composite;  biocompatibility 3T3 cells;  proliferation;  cytotoxicity;  cell adhesion
分类号:
TB 332；Q 2-33
DOI:
10.3724/SP.J.1249.2013.04392
文献标志码:
A
摘要:
聚醚醚酮（polyetheretherketone, PEEK）与纳米羟基磷灰石（hydroxyapatite, HA）共混制备PEEK-HA生物复合材料. 通过倒置荧光显微镜观察材料对细胞形态影响，不同HA含量的PEEK-HA复合材料中活细胞数目与空白对照无明显差异. 采用噻唑蓝（3-（4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide，MTT）染色法研究细胞增殖的作用，发现不同HA含量的PEEK-HA复合材料的质量浓度为4 mg/mL时，该生物复合材料对细胞增殖有一定促进作用；8～64 mg/mL时，对细胞增殖作用逐渐减小；64 mg/mL时，对细胞增殖作用最小，表明随着材料质量浓度增加可抑制细胞增殖. 测定PEEK-HA生物复合材料材料对3T3细胞黏附率，发现随着HA含量的增加，细胞黏附率增大，HA可以改善材料黏附性能. PEEK-HA 生物复合材料细胞毒性级别均在Ⅰ级，说明对3T3细胞无毒性.
Abstract:
On the basis of preparing polyetheretherketone-hydroxyapatite (PEEK-HA) composite materials, this paper focuses on the effect of the composite materials on 3T3 cells proliferation at different periods and under different concentrations. Based on the polycondensation of polyether-ether-ketone, the biological material was prepared by adding 10%, 20% and 30% hydroxyapatite blending into PEEK, respectively. The cell morphology 3T3 cell adhesion rate of the material was determined by cell counting with inverted fluorescence microscope. The MTT (4, 5-Dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide) method was used to study cell proliferation in various conditions. Results show that, in a concentration of 4 mg/mL，the material played a significant role in cell proliferation, and, in a concentration of 64 mg/mL, the effect of PEEK composites on cell proliferation is slight. In addition, cell adhesion rate increases with an increase of the HA content in PEEK-HA materials. The content of HA can improve cell adhesion to the composite materials. Cytotoxicity of the materials is found at levelⅠ, indicating that PEEK-HA composites are non-toxic to 3T3 cells.

参考文献/References:

［1］ Zhang Fang，Cui Chunxiang，Qi Yumin. Studies on cultured osteoblast cells on HA/Ti substitute for bone tissue［J］. Journal of Functional Materials,2005,36(5):740-742.(in Chinese)
张芳, 崔春翔, 戚玉敏.羟基磷灰石/钛合金骨替代材料体外培养成骨细胞的实验研究［J］.功能材料,2005,36(5):740-742.
［2］ Hou Wenming. The atomic absorption spectroscopy measurement of Ni ion release rate of TiNi and 317L alloys［J］. Journal of Biomedical Engineering,1995,12(2):182-185.(in Chinese)
侯文明. 羟基磷灰石复合材料的复合作用研究［J］. 生物医学工程杂志，1995，12(2):182-185.
［3］ Yan Han, Yang Yanhui, Xu Jing, et al. Research and application of nano-hydroxyapatite composites in hard tissue engineering［J］. Chemical Research and Application,2012,24(1):16-20.(in Chinese)
严涵，杨延慧，徐静,等.纳米羟基磷灰石复合材料在硬组织工程领域中的应用研究［J］. 化学研究与应用,2012,24(1):16-20.
［4］ Sang Peiming, Zhang Ming. Research status and advances of nano-hydroxyapatite/polyamide66 composite［J］. Medical Recapitulate,2012,18(13):2072-2074.(in Chinese)
桑裴铭,张明.医用纳米羟基磷灰石/聚酰胺66复合生物材料研究现状及进展［J］.医学综述，2012，18(13)：2072-2074.
［5］ Jockish K A, Brown S A, Bauer T W, et al. Biological response to chopped carbon reinforced PEEK［J］.Journal of Biomedical Materials Research, 1992, 26(2): 133-146.
［6］ Bakar M，Cheang P，Khor K, Mechanical properties of injection molded hudroxyapatite-polyetheretherketone biocomposites［J］. Composites Science and Technology, 2003,63:421-425.
［7］ Yu S,Hariraw K P,Kumar R. In vitro apatite formation and its growth kinetics on hydroxyapatite/polyetheretherketione biocomposites［J］.Biomaterials，2005，26（15）：2343-2352.
［8］ Xiao Yun. Biomedical materials research progress［J］. Journal of Wuhan Bioengineering Institute, 2007， 3(3)：183-186.(in Chinese)
肖云. 生物医学材料研究进展［J］. 武汉生物工程学院学报，2007，3(3)：183-186.
［9］ Zhao Chun, Zhang Yulong. Polyetheretherketone［M］.Beijing: Chemical Industry Press,2008.(in Chinese)
赵纯，张玉龙. 聚醚醚酮［M］. 北京：化学工业出版社，2008.
［10］ Feng Xing, Sui Guoxin,Yang Rui. Mechanical properties and in vitro bioactivity of carbon fibre-reinforced polyetheretherketone-hydroxyapatite(PEEK-HA)composites for biomedical applications［J］. Chinese Journal of Materials Research，2008,22(1):18-25.(in Chinese)
冯惺，隋国鑫，杨锐. PEEK-HA-CF 复合材料的力学性能和体外生物活性［J］. 材料研究学报，2008,22(1):18-25.
［11］ Xue Limin, Zhao Jianfeng, Wu Jiefeng. et al. Synthesis and characterization of PEEK［J］. New Chemical Materials, 2007,35(1):54-56.(in Chinese)
徐利敏,赵剑锋,吴结丰,等.PEEK的合成与表征［J］.化工新型材料,2007,35(1): 54-56.
［12］ Cai Weiquan，Weng Luqian，Ni Zhuo，et al. In situ polymerization of hydroxyapatite/poly(ether-ether-ketone) composites［J］. New Chemical Materials, 2010，38（6）：37-40.(in Chinese)
蔡卫全，翁履谦，倪卓,等. 原位聚合羟基磷灰石/聚醚醚酮复合材料［J］. 化工新型材料，2010，38(6)：37-40.
［13］ Ni Zhuo，Wang Ying，Liu Shide，et al. Thermal stability of PEEK/HA biological composite materials［J］. Journal of Shenzhen University Science and Engineering, 2012,29(6)：521-526.(in Chinese)
倪卓，王应，刘士德，等.PEEK/HA生物复合材料的热稳定性［J］. 深圳大学学报理工版，2012,29（6）：521-526.
［14］ Ni Zhuo，Tian Shengli，Wang Ying，et al. PEEK/HA composite materials biocompatibility［J］. Orthopaedic Biomechanics Materials and Clinical Study, 2012,9(4)：9-12.(in Chinese)
倪卓，田生礼，王应，等.PEEK/HA复合材料热稳定性及细胞毒性试验［J］.生物骨科材料与临床研究，2012,9(4)：9-12.
［15］ Quan Renfu，Yang Disheng，Miao Xudong. Biocompatibility of graded zirconia-hydroxyapatite composite［J］. Chinese Journal of Reparative and Reconstructive Surgery, 2006，20(5)：569-574.(in Chinese)
全仁夫，杨迪生，苗旭东. 二氧化锆梯度复合羟基磷灰石的生物相容性研究［J］. 中国修复重建外科杂志，2006，20(5)：569-574.
［16］ Zhao Ming，Zheng Qixin，Wang Jinguang，et al. Effect of biomimetic mineralization of PLGA on the adhesion rate of bone mesenchymal stem cells［J］. International Journal of Biomedical Engineering, 2006，29(1)：1-4.(in Chinese)
赵铭，郑启新，王金光，等. PLGA仿生矿化对骨髓基质干细胞黏附率的影响［J］. 国际生物医学工程杂志，2006，29(1)：1-4.
［17］ Wan Changxiu. Biological Properties of the Material and its Evaluation［M］. Chengdu: Sichuan University Press,2008.(in Chinese)
万昌秀. 材料的生物学性能及其评价［M］. 成都：四川大学出版社，2008.

相似文献/References:

[1]倪　卓,刘剑洪,魏波,等.PEEK/AS4复合材料微观结构表征[J].深圳大学学报理工版,2007,24(1):47.
　NI Zhuo,LIU Jian-hong,WEI Bo,et al.Microstructural charaterization of Polyetheretherketon/AS4 composites[J].Journal of Shenzhen University Science and Engineering,2007,24(No.4(331-440)):47.
[2]曹慧群,周晓明,刘剑洪,等.氧化铕组装碳纳米管的制备和表征[J].深圳大学学报理工版,2007,24(1):54.
　CAO Hui-qun,ZHOU Xiao-ming,LIU Jian-hong,et al.The preparation and characteration of carbon nanotubes assembled with europium oxide[J].Journal of Shenzhen University Science and Engineering,2007,24(No.4(331-440)):54.
[3]倪卓,张萍,林燕玲,等.不饱和聚酯/微胶囊复合材料自修复性能[J].深圳大学学报理工版,2010,27(3):260.
　NI Zhuo,ZHANG Ping,LIN Yan-ling,et al.Self-repairing behaviours of unsaturated polyester/microcapsule composites[J].Journal of Shenzhen University Science and Engineering,2010,27(No.4(331-440)):260.
[4]贵大勇,郝景峰,赖玉丽,等.有机硅改性环氧树脂制备及热稳定性[J].深圳大学学报理工版,2011,28(No.6(471-564)):507.
　GUI Da-yong,HAO Jing-feng,LAI Yu-li,et al.Modification of epoxy resin with diphenyl silanediol and its thermal stability[J].Journal of Shenzhen University Science and Engineering,2011,28(No.4(331-440)):507.
[5]许新统,翟剑庞,付玲,等.CHA晶体孔道内Se8合成及光学表征[J].深圳大学学报理工版,2011,28(No.6(471-564)):518.
　XU Xin-tong,ZHAI Jian-pang,FU Ling,et al.Synthesis and optical characterization of Se8ring incorporated inside the cages of CHA crystals[J].Journal of Shenzhen University Science and Engineering,2011,28(No.4(331-440)):518.
[6]宋远佳,张炜,杨正伟,等.固体火箭发动机壳体脱黏缺陷的热波检测[J].深圳大学学报理工版,2012,29(No.3(189-282)):252.[doi:10.3724/SP.J.1249.2012.03252]
　SONG Yuan-jia,ZHANG Wei,YANG Zheng-wei,et al.Debond defect detection in shell of solid rocket motor by thermal wave nondestructive testing[J].Journal of Shenzhen University Science and Engineering,2012,29(No.4(331-440)):252.[doi:10.3724/SP.J.1249.2012.03252]
[7]倪卓,王应,华文语,等.PEEK/HA生物复合材料的热稳定性[J].深圳大学学报理工版,2012,29(No.6(471-580)):521.[doi:10.3724/SP.J.1249.2012.06521]
　NI Zhuo,WANG Ying,HUA Wen-yu,et al.Thermal stability of ?PEEK/HA biological composite materials[J].Journal of Shenzhen University Science and Engineering,2012,29(No.4(331-440)):521.[doi:10.3724/SP.J.1249.2012.06521]
[8]罗园春,朱光明,汤皎宁,等.环氧树脂及其固化剂对生物微胶囊性能的影响[J].深圳大学学报理工版,2015,32(4):371.[doi:10.3724/SP.J.1249.2015.04371]
　Luo Yuanchun,Zhu Guangming,Tang Jiaoning,et al.Influence of epoxy resin and its curing agent on the properties of biomicrocapsule[J].Journal of Shenzhen University Science and Engineering,2015,32(No.4(331-440)):371.[doi:10.3724/SP.J.1249.2015.04371]
[9]倪卓,曾茵茵,周方宇,等.碳纳米管对有机相变储能材料的影响[J].深圳大学学报理工版,2016,33(3):241.[doi:10.3724/SP.J.1249.2016.03241]
　Ni Zhuo,Zeng Yinyin,Zhou Fangyu,et al.Effect of carbon nanotubes on organic phase change materials[J].Journal of Shenzhen University Science and Engineering,2016,33(No.4(331-440)):241.[doi:10.3724/SP.J.1249.2016.03241]
[10]倪卓,杨莎,吴耿峰,等.PES-HA生物复合材料的热稳定性和生物毒性[J].深圳大学学报理工版,2017,34(3):313.[doi:10.3724/SP.J.1249.2017.03313]
　Ni Zhuo,Yang Sha,Wu Gengfeng,et al.Thermal stability and biotoxicity of PES-HA biocomposites[J].Journal of Shenzhen University Science and Engineering,2017,34(No.4(331-440)):313.[doi:10.3724/SP.J.1249.2017.03313]
[11]倪卓,王应,王双,等.PEEK-HA生物复合材料对MG-63细胞的作用[J].深圳大学学报理工版,2014,31(3):258.[doi:10.3724/SP.J.1249.2014.03258]
　Ni Zhuo,Wang Ying,Wang Shuang,et al.Influence of PEEK-HA composites on MG-63 cells[J].Journal of Shenzhen University Science and Engineering,2014,31(No.4(331-440)):258.[doi:10.3724/SP.J.1249.2014.03258]
[12]倪卓,杨莎,王应,等.骨替代材料的研究方法及进展[J].深圳大学学报理工版,2015,32(4):331.[doi:10.3724/SP.J.1249.2015.04331]
　Ni Zhuo,Yang Sha,Wang Ying,et al.Research methods and advances on bone substitute materials[J].Journal of Shenzhen University Science and Engineering,2015,32(No.4(331-440)):331.[doi:10.3724/SP.J.1249.2015.04331]

备注/Memo

备注/Memo:
Received：2013-03-19；Accepted：2013-06-05
Foundation：Guangdong Province Innovative Team Fund (9351806001000001)
Corresponding author：Professor Ni Zhuo. E-mail：royzhuoni@hotmail.com
Citation：Ni Zhuo, Liu Shide, Wang Ying, et al. Influence of 3T3 cells for PEEK-HA composite［J］. Journal of Shenzhen University Science and Engineering, 2013, 30(4): 392-397.（in Chinese）
基金项目：广东省创新团队基金资助项目(9351806001000001)
作者简介：倪卓（1963-），男（汉族），吉林省通化市人，深圳大学教授、博士生导师. E-mail：royzhuoni@hotmail.com
引文：倪卓，刘士德，王应，等. PEEK-HA生物材料对成纤维细胞3T3的影响［J］. 深圳大学学报理工版，2013，30（4）：392-397.
更新日期/Last Update: 2013-07-12