TANG Yu-lin,CAO Yan,OU Zhong-hua,et al.Regulatable gene expression controlled by the promoter of Sali3-2 under different abiotic stresses[J].Journal of Shenzhen University Science and Engineering,2012,29(No.1(001-094)):73-79.[doi:10.3724/SP.J.1249.2012.01073]





Regulatable gene expression controlled by the promoter of Sali3-2 under different abiotic stresses
1) 深圳大学生命科学学院,深圳 518060
2) 深圳市海洋生物资源与生态环境重点实验室,深圳市微生物基因工程重点实验室,深圳 518060
TANG Yu-lin12 CAO Yan12 OU Zhong-hua12 YANG Xi-ni12 HU Lian-ping12 and ZHENG Yi-zhi12
1) College of Life Science, Shenzhen University, Shenzhen 518060, P.R.China
2) The Key Laboratory for Marine Bioresource and Eco-environmental Science, Shenzhen Key Laboratory of Microbial and Gene Engineering, Shenzhen 518060, P.R.China
bioinformatics gene regulation promoter Sali3-2 copper response elements abiotic stress GUS analysis
Q 78; Q 812
通过生物信息学方法对Sali3-2基因上游非编码区序列(-1 945 /+1)中的顺式作用元件进行预测.发现该序列中存在铜响应元件、干旱胁迫响应元件及与低温等非生物胁迫相关元件等.在不同非生物胁迫条件下,分别对转基因烟草悬浮细胞和转基因拟南芥幼苗中Sali3-2启动子片段驱动报告基因β-葡萄糖苷酸酶(β-glucuronidase,Gus)的表达进行分析.结果表明,在转基因烟草细胞和拟南芥幼苗中, Gus基因的表达明显受Al3+胁迫的诱导和Cu2+过多的抑制;在转基因烟草细胞中,该基因的表达一定程度上受脱落酸、渗透胁迫和盐胁迫的诱导,研究结果为了解Sali3-2基因的功能及调控作用奠定了基础.
Sali3-2 from soybean is a BURP gene related to stress response. To elucidate its transcriptional regulation characteristics, the upstream non-coding sequence (-1 945 /+1) of Sali3-2 was scanned in PLACE database for searching the cis-acting regulatory elements. Several copper response elements and some cis-element motifs related to gene induction by abscisic acid (ABA), dehydration and cold stress were found in this sequence. The expression of reporter gene Gus driven by the promoter region of Sali3-2 was further studied in tobacco cells and Arabidopsis seedlings at different abiotic stresses. Results show that the expression of the reporter is obviously up-regulated by Al3+ stress and intensely suppressed by abundance Cu2+. The expression of the reporter is slightly up-regulated by abscisic acid, osmotic stress and salt stress in transgenic tobacco cells. These findings extend our understanding of the regulation of Sali3-2 expression.


[1] Hattori J, Boutilier K A, van Lookeren Campagne M M, et al. A conserved BURP domain defines a novel group of plant proteins with unusual primary structures[J].Molecular and General Genetics, 1998, 259(4): 424-428.
[2] TANG Yu-lin, WANG Ya-jing, CAI Xue-mei, et al. A BURP-domain family of plant proteins[J]. Progress in Natural Science, 2009,19 (3): 241-247.(in Chinese)
唐玉林,王亚静,蔡雪梅,等. 植物BURP-蛋白家族[J]. 自然科学进展, 2009; 19 (3): 241-247.
[3] Shao Y, Wei G, Wang L, et al. Genome-wide analysis of BURP domain-containing genes in Populus trichocarpa[J]. Journal of Integrative Plant Biology, 2011, 53 (9) : 743-755.
[4] WANG Ai-ming, XIA Qun, XIE Wen-shuang, et al. The classical Ubisch bodies carry a sporophytically produced structural protein (RAFTIN) that is essential for pollen development[J]. Proceedings of the National Academy of Sciences of the United States of America, 2003, 100(24): 14487-14492.
[5] Ding X, Hou X, Xie K, et al. Genome-wide identification of BURP domain-containing genes in rice reveals a gene family with diverse structures and responses to abiotic stresses[J]. Planta, 2009, 230(1): 149-163.
[6] Yamaguchi-Shinozaki K, Shinozaki K. The plant hormone abscisic acid mediates the drought-induced expression but not the seed-specific expression of rd22, a gene responsive to dehydration stress in Arabidopsis thaliana[J]. Molecular and General Genetics MGG, 1993, 238(1/2): 17-25.
[7] Yu S, Zhang L, Zuo K, et al. Isolation and characterization of a BURP domain-containing gene BnBDC1 from Brassica napus involved in abiotic and biotic stress[J]. Physiologia Plantarum, 2004, 122(2): 210-218.
[8] Banzai T, Sumiya K, Hanagata N, et al. Molecular cloning and characterization of genes encoding BURP domain-containing protein in the mangrove, Bruguiera gymnorrhiza[J]. Trees-Structure and Function, 2002, 16(2): 87.
[9] Ragland M, Soliman K M. Two genes induced by Al in soybean roots[J]. Plant Physiol, 1997, 114: 395-396.
[10] TANG Yu-lin, LI Xiao-jie, ZHONG Yun-ting, et al. Functional analysis of soybean SALI3-2 in yeast[J]. Journal of Shenzhen University Science and Engineering, 2007, 24(3): 324-330. (in Chinese)
唐玉林, 李小杰, 钟昀廷, 等. 利用酵母表达体系研究大豆SALI3-2蛋白功能[J]. 深圳大学学报理工版, 2007, 24(3): 324-330.
[11] Higo K,Ugawa Y,Iwamoto M,et al.Plant cis-acting regulatory DNA elements (PLACE) database[J]. Nucleic Acids Research, 1999, 27 (1): 297-300.
[12] Clough S J, Bent A F. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana[J]. The Plant Journal, 1998, 16(6): 735-743.
[13] Jefferson R A, Kavanagh T A, Bevan M W. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants[J]. The EMBO Journal, 1987, 6(13): 3901-3907.
[14] Campbell M, Segear E, Beers L, et al. Dormancy in potato tuber meristems: chemically induced cessation in dormancy matches the natural process based on transcript profiles[J]. Funct Integr Genomics, 2008, 8(4): 317-328.
[15] Abe H, Yamaguchi-Shinozaki K, Urao T, et al. Role of arabidopsis MYC and MYB homologs in drought- and abscisic acid-regulated gene expression[J]. Plant Cell, 1997, 9(10): 1859-1868.
[16] Quinn J M, Barraco P, Eriksson M, et al. Coordinate copper-and oxygen-responsive Cyc6 and Cpx1 expression in Chlamydomonas is mediated by the same element[J]. The Journal of Biological Chemistry, 2000, 275(9): 6080-6089.
[17] Kropat J, Tottey S, Birkenbihl R P, et al. A regulator of nutritional copper signaling in Chlamydomonas is an SBP domain protein that recognizes the GTAC core of copper response element[J]. Proceeding of the National Academy of Sciences, 2005, 102(51): 18730-35.


[1]夏立新,闫浩,汤慕瑾,等.花生过敏原Ara h2与Ara h6的生物信息学比较研究[J].深圳大学学报理工版,2010,27(2):241.
 XIA Li-xin,YAN Hao,TANG Mu-jin,et al.Bioinformatics comparison of peanut allergen Ara h2 and Ara h6[J].Journal of Shenzhen University Science and Engineering,2010,27(No.1(001-094)):241.
 BI De,XIAO Hua-jun,ZHOU Cui-hong,et al.Bioinformatic analysis of ?cardiac and pulmonary hypoxia[J].Journal of Shenzhen University Science and Engineering,2012,29(No.1(001-094)):541.[doi:10.3724/SP.J.1249.2012.06541]
 Hu Zhangli,Li Jiancheng,and Cheng Xuewei.The mitochondrial genetic system and exogenous gene expression of Chlamydomonas reinhardtii[J].Journal of Shenzhen University Science and Engineering,2013,30(No.1(001-094)):603.[doi:10.3724/SP.J.1249.2013.06603]
 Chen Mengzhu,Wang Xiujie,and Xia Lixin.Comparison of alternative splicing in lung adenocarcinoma and lung squamous carcinoma[J].Journal of Shenzhen University Science and Engineering,2017,34(No.1(001-094)):33.[doi:10.3724/SP.J.1249.2017.01033]
 LOU Sulin,ZHU Xiulan,ZENG Zhiyong,et al.Bioinformatics analysis of Dunaliella microRNAs by high-throughput sequencing[J].Journal of Shenzhen University Science and Engineering,2018,35(No.1(001-094)):331.[doi:10.3724/SP.J.1249.2018.04331]
[6]张尉欣,刘阳,刘 帅,等.镉胁迫下菜心的转录组分析[J].深圳大学学报理工版,2018,35(5):543.[doi:10.3724/SP.J.1249.2018.05543]
 ZHANG Weixin,LIU Yang,LIU Shuai,et al.Transcriptome analysis of Brassica rapa ssp. chinensis var. parachinensis under cadmium stress[J].Journal of Shenzhen University Science and Engineering,2018,35(No.1(001-094)):543.[doi:10.3724/SP.J.1249.2018.05543]
 ZHONG Shan,WANG Yun,et al.The prediction of molecular functions for three novel miRNAs in esophageal squamous cell carcinoma[J].Journal of Shenzhen University Science and Engineering,2019,36(No.1(001-094)):347.[doi:10.3724/SP.J.1249.2019.04347]
 XU Zhouheng,ZHU Yingli,SUN Mengfei,et al.Comparative transcriptomic analysis of mouse striatum and retina[J].Journal of Shenzhen University Science and Engineering,2020,37(No.1(001-094)):17.[doi:10.3724/SP.J.1249.2020.01017]
[9]陈扬,陈献雄,欧阳春艳,等.粉尘螨过敏原Der f 35的克隆表达及免疫学鉴定[J].深圳大学学报理工版,2021,38(3):301.[doi:10.3724/SP.J.1249.2021.03301]
 CHEN Yang,CHEN Xianxiong,OUYANG Chunyan,et al.Cloning,expression and immunological identification of Der f 35 allergen from Dermatophagoides farinae[J].Journal of Shenzhen University Science and Engineering,2021,38(No.1(001-094)):301.[doi:10.3724/SP.J.1249.2021.03301]
 YANG Lei,WANG Yun,DENG Fang,et al.Circular RNAs related to skeletal muscle atrophy in diabetic mouse model[J].Journal of Shenzhen University Science and Engineering,2022,39(No.1(001-094)):262.[doi:10.3724/SP.J.1249.2022.03262]


Foundation:National Natural Science Foundation of China (30770184)
Corresponding author:Professor ZHENG Yi-zhi. E-mail: yzzheng@szu.edu.cn
Citation:TANG Yu-lin, CAO Yan, OU Zhong-hua, et al. Regulatable gene expression controlled by the promoter of Sali3-2 under different abiotic stresses[J]. Journal of Shenzhen University Science and Engineering, 2012, 29(1):73-79.(in Chinese)
作者简介:唐玉林(1968-),女(汉族),广西壮族自治区桂林市人,深圳大学副教授、博士. E-mail: yltang@szu.edu.cn
引文:唐玉林, 曹雁, 欧忠华, 等. 非生物胁迫因子对大豆Sali3-2基因的调控作用[J]. 深圳大学学报理工版,2012,29(1):73-79.
更新日期/Last Update: 2012-01-06