[1]莫蓓莘,姜里文.从烟草悬浮细胞中分离液泡前体的方法研究[J].深圳大学学报理工版,2005,22(2):109-112.
　MO Bei-xin and JIANG Li-wen.Isolation of prevacuolar compartments from tobacco suspension cells[J].Journal of Shenzhen University Science and Engineering,2005,22(2):109-112.
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从烟草悬浮细胞中分离液泡前体的方法研究()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第22卷
期数:
2005年2期
页码:
109-112
栏目:
生命科学
出版日期:
2005-04-30

文章信息/Info

Title:
Isolation of prevacuolar compartments from tobacco suspension cells
文章编号:
1000-2618(2005)02-0109-04
作者:
莫蓓莘1;  姜里文2
1）深圳大学生命科学学院, 深圳 518060；
2）香港中文大学生物系，香港
Author(s):
MO Bei-xin1 and JIANG Li-wen2
1)College of Life Science Shenzhen University ，Shenzhen 518060 P. R. China
2)Department of Biology The Chinese University of Hong Kong，Hong Kong
关键词:
液泡前体;  蛋白分泌途径;  蔗糖密度梯度离心;  烟草悬浮细胞;  纤维素酶;  果胶酶;  原生质体;  免疫印迹法
Keywords:
Prevacuolar compartment;  protein secretory pathway;  sucrose density gradient ultra-centrifugation;  tobacco suspension cells;  cellulase;  pectinase;  protoplast;  western blot
分类号:
Q 291
文献标志码:
A
摘要:
通过跟踪含有液泡前体特异标记物的组分, 利用细胞壁酶水解胞壁制造原生质体, 并用真针头挤压的方法裂解细胞, 用蔗糖密度梯度离心的方法分离液泡前体. 通过相差显微镜观察, 发现所分离的液泡前体保持完整的结构. 利用不同细胞器的标记物抗体进行免疫标记检测, 证明所纯化的液泡前体不含其他细胞器, 具有较高的纯度.
Abstract:
Pure and intact prevacuolar(PVC) compartments were isolated from tabacco suspension cells. The technique involved enzymes to digest the cell wall, releasing cell contents, which were scavenged by syringe. The PVC was then isolated using sucrose gradient ultracentrifugation. The organization of the isolated PVC fractions remained intact as observed by differential interference contrast microscopy. Western blots using markers for different organelles proved that the isolated PVC fractions have high purity.

相似文献/References:

[1]莫蓓莘,姜里文.烟草液泡前体的分离与超微结构研究[J].深圳大学学报理工版,2005,22(3):248.
　MO Bei-xin,JIANG Li-wen.Identification of multivesicular bodies structure for prevacuolar compartments[J].Journal of Shenzhen University Science and Engineering,2005,22(2):248.

更新日期/Last Update: 2015-10-16