[1]张建华,刘士德,刑苗.多头绒泡菌G2期cDNA表达文库的构建[J].深圳大学学报理工版,2005,22(1):50-56.
　ZHANG Jian-hua,LIU Shi-de,and XING Miao.Construction of a cDNA expression library of Physarum polycephalum at G2phase[J].Journal of Shenzhen University Science and Engineering,2005,22(1):50-56.
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多头绒泡菌G2期cDNA表达文库的构建()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第22卷
期数:
2005年1期
页码:
50-56
栏目:
生命科学
出版日期:
2005-01-30

文章信息/Info

Title:
Construction of a cDNA expression library of Physarum polycephalum at G2phase
文章编号:
1000-2618(2005)01-0050-07
作者:
张建华; 刘士德; 刑苗
深圳大学生命科学学院, 深圳市微生物基因工程重点实验室, 深圳 518060
Author(s):
ZHANG Jian-hua;  LIU Shi-de;  and XING Miao
Shenzhen Key Laboratory of Microbial Gene Engineering, College of Life Science Shenzhen University Shenzhen 518060 P .R. China
关键词:
多头绒泡菌;  cDNA表达文库;  SC35类似蛋白
Keywords:
Physarum polycephalum;  cDNA expression library;  SC35 like protein
分类号:
Q7
文献标志码:
A
摘要:
cDNA表达文库是分离、筛选基因的重要平台.为分离多头绒泡菌SC35类似蛋白PSCL32.5及其激酶的基因,首先检测多头绒泡菌PSCL32.5及其激酶在细胞周期不同时相的含量,发现PSCL32.5在G2期含量最高;再从多头绒泡菌G2期RNA出发,构建多头绒泡菌G2期cDNA表达文库.得到cDNA文库的初始滴度为1.22×106pfu/mL(pfu为噬菌体数目),cDNA扩增文库的滴度为1.12×1011pfu/mL,插入片段的重组率接近100%,插入片段的大小在0.5～2.5kb范围,说明所构建的cDNA文库质量较好.
Abstract:
In order to isolate the genes of SC35-like proteins and SRPK-like proteins of Physarum polycephalum, a cDNA expression library of P. polycephalum at G2 phase was constructed. The titer of the primary cDNA library is1.22×106 pfu/mL and of the amplified library is 1.12×1011pfu/mL?(plaque-forming units, pfu). Almost all of the phages in the cDNA library were reconstituted. The size of the inserts ranges from0.5 kb to 2.5 kb. All of the above mentioned have answered for the general requirements of a cDNA expression library.

相似文献/References:

[1]刘士德,陈汗英,张建华,等.PSTI1可提高大肠杆菌应答胁迫能力[J].深圳大学学报理工版,2011,28(No.4(283-376)):347.
　LIU Shi-de,CHEN Han-ying,ZHANG Jian-hua,et al.A novel STI1-like protein from Physarum polycephalum can enhance E.coli response to stress[J].Journal of Shenzhen University Science and Engineering,2011,28(1):347.

更新日期/Last Update: 2015-10-16