[1]王玳玮,王筠,赵宋礼,等.消化系统多种癌变组织中3种蛋白质的表达谱[J].深圳大学学报理工版,2022,39(3):253-261.[doi:10.3724/SP.J.1249.2022.03253]
 WANG Daiwei,WANG Yun,ZHAO Songli,et al.Expression profiles of three proteins in various cancerous tissues of digestive system[J].Journal of Shenzhen University Science and Engineering,2022,39(3):253-261.[doi:10.3724/SP.J.1249.2022.03253]
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消化系统多种癌变组织中3种蛋白质的表达谱()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第39卷
期数:
2022年第3期
页码:
253-261
栏目:
生命与健康
出版日期:
2022-05-16

文章信息/Info

Title:
Expression profiles of three proteins in various cancerous tissues of digestive system
文章编号:
202203003
作者:
王玳玮1王筠1赵宋礼2曾健1胡铭慧1许特1江波涛13董蕾3盛司潼1
1)深圳大学生命与海洋科学学院,广东深圳 518071
2)佛山市三水区人民医院,广东佛山 528100
3)西安交通大学第二附属医院,陕西西安 710003
Author(s):
WANG Daiwei1WANG Yun1ZHAO Songli2ZENG Jian1HU Minghui1XU Te1JIANG Botao13DONG Lei3and SHENG Sitong1
1) College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518071, Guangdong Province, P. R. China
2) Sanshui District People’s Hospital, Foshan 528100, Guangdong Province, P. R. China
3) The Second Affiliated Hospital, Xi’an Jiaotong University, Xi’an 710003, Shaanxi Province, P. R. China
关键词:
分子诊断消化道肿瘤蛋白质表达谱恶性肿瘤多重免疫组织化学染色stomatin家族蛋白1stomatin家族蛋白2TATA序列结合蛋白相关因子1
Keywords:
molecular diagnosis gastrointestinal cancer protein expression profiling malignant tumors multiple immunohistochemical staining stomatin-like protein 1 (STOML1) stomatin-like protein 2 (STOML2) TATA-box binding protein associated factor 1 (TAF1L)
分类号:
R363.1;R735
DOI:
10.3724/SP.J.1249.2022.03253
文献标志码:
A
摘要:
在口腔、食管和结直肠癌病灶中,TATA序列结合蛋白相关因子1(TATA-box binding protein associated factor 1 like,TAF1L)、stomatin家族蛋白1(stomatin-like protein 1, STOML1)和stomatin家族蛋白2(stomatin-like protein 2, STOML2)存在异常表达.为探究它们在消化系统癌变过程中的作用机理,利用6种消化道肿瘤(食道、胃、结肠、直肠、肝脏和胰腺)及癌旁组织的微陈列芯片,通过多重免疫组织化学染色,比对分析TAF1L、STOML1和STOML2这3种蛋白质在癌与癌旁组织的差异表达及与癌变的关联.结果发现,STOML2在食道、胃、结肠、直肠、肝脏和胰腺这6种消化系统癌组织中均呈显著性高表达(P<0.001),而同一家族成员STOML1则仅在食管癌和肝癌组织中表达明显增高(P<0.005).另外,除胰腺癌灶外,TAF1L在其余5种消化系统癌组织中也呈现高表达(P<0.005).同时,TAF1L、STOML1和STOML2这3种蛋白质均与上皮间充质转化的关键蛋白质MMP9有共定位现象.由此表明,3种蛋白质TAF1L、STOML1和STOML2在多种癌组织中可能具有广泛的促癌作用.由于3种蛋白质在多种癌组织的表达水平不同,说明它们各自存在着组织特异性,且具有不同的功能使命,需进一步研究TAF1L、STOML1和STOML2这3种蛋白质在消化系统恶性肿瘤发生发展过程中各自的分子病理学潜能,如能将它们打造成用于精准诊疗和预后判断的新型分子标志物,此研究结果将有重要的临床意义.
Abstract:
A variety of gastrointestinal tumors are the most malignancies with high morbidity and mortality globally. The previous studies show that there are abnormal expressions of TATA-box binding protein associated factor 1 like (TAF1L), stomatin-like protein 1 (STOML1) and stomatin-like protein 2 (STOML2) genes in oral, esophageal and colorectal cancer lesions. To explore the potential roles of above three genes in the carcinogenesis of digestive system, the differential expressions of TAF1L, STOML1 and STOML2 in cancerous and paracancerous tissues are compared and analyzed by multiple immunohistochemical staining using microarray of 6 cancer tissues of esophagus, stomach, liver, pancreas, colon and rectum with matched paracancerous tissues. The results show that STOML2 is significantly overexpressed in all of 6 gastrointestinal cancers (P<0.001), and STOML1, one of the same family member of STOML2, is highly expressed only in esophageal and liver cancers (P<0.005). In addition, except for pancreatic cancer, TAF1L is also abnormal highly expressed in other 5 cancer tissues (P<0.005). At the same lesion tissue section, STOML1, STOML2 and TAF1L proteins are colocated with the key protein MMP9 of epithelial-mesenchymal transformation in positive cells. This study indicates that the three proteins of TAF1L, STOML1 and STOML2 might have a wide range of cancer promoting effects in multiple cancer tissues in the digestive system.In addition, due to their different expression levels in a variety of cancer tissues, they have tissue specificity and different functional missions.Further exploration of the molecular pathological potential of TAF1L, STOML1 and STOML2 in the occurrence and development of malignant tumors in digestive system can make them serve as new molecular markers for early diagnosis, differential diagnosis, targeted therapy and prognosis prediction in multiple digestive tumors.

参考文献/References:

[1] SIEGEL R, MILLER K, JEMAL A.Cancer statistics [J].CA: A Cancer Journal for Clinicians, 2019, 69(1): 7-34.
[2] 李辰飞,陈星.生物标记物在胃癌筛查中的研究进展及临床应用价值[J].中国药物与临床,2018,18(6):924-927.
LI Chenfei, CHEN Xing. Research progress and clinical application value of biomarkers in gastric cancer screening [J].Chinese Remedies and Clinics, 2018, 18(6): 924-927.(in Chinese)
[3] SATHIYASEKAR A C, CHANDRASEKAR P, PAKASH A, et al.Overview of immunology of oral squamous cell carcinoma [J].Journal of Pharmacy and Bioallied Sciences, 2016, 8(Suppl.1): 8-12.
[4] ZHANG Qu, ZHANG Jun, JIN Hong, et al.Whole transcriptome sequencing identifies tumor-specific mutations in human oral squamous cell carcinoma [J].BMC Medical Genomics, 2013, 6(28): 1-7.
[5] WANG Daiwei, QI Hong, ZHANG Haoxing, et al. TAF1L promotes development of oral squamous cell carcinoma via decreasing autophagy-dependent apoptosis [J].International Journal of Biological Sciences, 2020, 16(7): 1180-1193.
[6] XU Te, WANG Yun, ZHAO Songli, et al. Two new molecular biomarkers in human colorectal cancer [J]. Journal of Shenzhen University Science and Engineering, 2018, 35: 529-535.
[7] ZHONG Shan, YAN Hongfei, CHEN Zhengshan, et al. Overexpression of TAF1L promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma [J]. Journal of Cancer, 2019, 10(4): 979-989.
[8] WANG Daiwei, QI Hong, LI Ang, et al. Coexisting overexpression of STOML1 and STOML2 proteins may be associated with pathology of oral squamous cell carcinoma [J]. Oral Surgery Oral Medicine Oral Pathology and Oral Radiology, 2020, 129(6): 591-599.
[9] ASIEDU M K, THOMAS C J, DONG J, et al. Pathways impacted by genomic alterations in pulmonary carcinoid tumors [J]. Clinical Cancer Research : an Official Journal of the American Association for Cancer Research, 2018, 24(7): 1691-1704.
[10] OH H R, AN C H, YOO N J, et al. Frameshift mutations in the mononucleotide repeats of TAF1 and TAF1L genes in gastric and colorectal cancers with regional heterogeneity [J].Pathology Oncology Research, 2017, 23(1): 125-130.
[11] NECCHI A, LO V S, MARIANI L,et al.An open-label, single-arm, phase 2 study of the Aurora kinase A inhibitor alisertib in patients with advanced urothelial cancer [J].Investigational New Drugs, 2016, 34(2): 236-242.
[12] ZHU Wenyu, WEI Li, QIAN Geng, et al. Silence of stomatin-like protein 2 represses migration and invasion ability of human liver cancer cells via inhibiting the nuclear factor kappa B (NF-κB) pathway [J].Medical Science Monitor, 2018, 24(25): 7625-7632.
[13] GOSNEY J A, WILKEY D W, MERCHANT M L, et al. Proteomics reveals novel protein associations with early endosomes in an epidermal growth factor-dependent manner [J]. The Journal of Biological Chemistry, 2018, 293(16): 5895-5908.
[14] ZHOU Chaxi, LI Yong, WANG Guiying, et al. Enhanced SLP-2 promotes invasion and metastasis by regulating Wnt/β-catenin signal pathway in colorectal cancer and predicts poor prognosis [J]. Pathology Research and Practice, 2019, 215(1): 57-67.
[15] GUO R L, WANG X R, WANG Q G, et al. The regulatory role of SLP-2 and mechanism on CCBE1 gene expression in rectal carcinoma and adjacent lymphatic tube tissues [J]. European Review for Medical and Pharmacological Sciences, 2018, 22(1): 87-94.
[16] 许特,王筠,邓芳,等.组织芯片多重免疫组织化学染色方法的优化[J].深圳大学学报理工版,2019,36(5):570-575.
XU Te, WANG Yun, DENG Fang, et al. Technique improvement of multiple immunohistochemical staining on tissue microarray [J]. Journal of Shenzhen University Science and Engineering, 2019, 36(5): 570-575.(in Chinese)
[17] WANG P J, PAGE D C. Functional substitution for TAF(II) 250 by a retroposed homolog that is expressed in human spermatogenesis [J]. Human Molecular Genetics, 2002, 11(19): 2341-2346.
[18] LAPATSINA L, BRAND J, POOLE Kate, et al. Stomatin-domain proteins [J]. European Journal of Cell Biology, 2012, 91(4): 240-245.
[19] ZHENG Yahui, HUANG Chong, LU Lu, et al. STOML2 potentiates metastasis of hepatocellular carcinoma by promoting PINK1-mediated mitophagy and regulates sensitivity to lenvatinib [J]. Journal of Hematology & Oncology, 2021, 14(1): 16.

备注/Memo

备注/Memo:
Received: 2021-02-20; Accepted: 2021-05-12; Online (CNKI): 2022-04-22
Foundation: National Key R & D Program of China (2016YFC1304000); Science Technology and Innovation Committee of Shenzhen Municipality (KCXFZ202002011006448); Opening Project of Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research (2016LHM-KFKT010, 2018LHM-KFKT007)
Corresponding author: Professor WANG Yun.E-mail: yunw@szu.edu.cn
Citation: WANG Daiwei, WANG Yun, ZHAO Songli, et al. Expression profiles of three proteins in various cancerous tissues of digestive system [J]. Journal of Shenzhen University Science and Engineering, 2022, 39(3): 253-261.(in Chinese)
基金项目:国家重点研发计划资助项目(2016YFC1304000);深圳市科技创新委员会可持续发展专项资助项目(KCXFZ 202002011006448);陕西省颅颌面精准医学研究重点实验室开放基金项目(2016LHM-KFKT010,2018LHM-KFKT007)
作者简介:王玳玮(1985—),深圳大学副研究员、博士.研究方向:慢性病早期筛查及诊断.E-mail: dweiwang@foxmail.com
引 文:引用格式:王玳玮,王筠,赵宋礼,等.消化系统多种癌变组织中3种蛋白质的表达谱[J].深圳大学学报理工版,2022,39(3):253-261.
更新日期/Last Update: 2022-05-30