HE Huan,XIE Ruolan,DING Senxu,et al.Screening of cadmium resistant bacteria and their effects on plant growth promotion and remediation in soil[J].Journal of Shenzhen University Science and Engineering,2021,38(4):374-379.[doi:10.3724/SP.J.1249.2021.04374]





Screening of cadmium resistant bacteria and their effects on plant growth promotion and remediation in soil
3)四川大学宜宾产业技术研究院,四川宜宾 644010
HE Huan1 XIE Ruolan1 DING Senxu1 and YU Jiang1 2 3
1) College of Architecture and Environment, Sichuan University, Chengdu 610065, Sichuan Province, P.R.China
2) Institute of New Energy and Low Carbon Technology, Sichuan University, Chengdu 610065, Sichuan Province, P.R.China
3) Yibin Institute of Industrial Technology, Sichuan University, Yibin 644010, Sichuan Province, P.R.China
biomaterials Cd Cd resistant bacteria soil physicochemical properties growth-promoting properties phytoextraction heavy metal pollution
为考察微生物-植物联合体系对土壤重金属镉(Cd)污染的修复效果,通过菌株筛选与盆栽实验,筛选一株具有较高Cd耐受和促生能力的菌株,探究植物促生菌对黑麦草富集能力、土壤理化性质和重金属赋存形态的影响.研究结果显示,伯克霍尔德氏菌株YXL1呈淡黄色、杆状、边缘圆滑,平均长度为5 μm,且耐受重金属镉的质量浓度达250 mg/L.YXL1产吲哚乙酸(indole-3-acetic acid, IAA)能力为31.56 mg/L,产铁载体能力为0.89,溶磷能力为46.18 mg/L.在YXL1与黑麦草的联合修复体系中,土壤的有机质和有效磷质量浓度均显著提高,分别增加了4.08 g/kg和2.54 mg/kg;土壤中弱酸可提取态Cd和还原态Cd的质量分数分别达16.25%和12.53%;与仅种植黑麦草的对照组相比,联合修复体系黑麦草的富集Cd能力提高了2.76倍.研究发现,以YXL1和黑麦草联合修复体系处理Cd污染农田土壤,4 a后耕作层土壤的Cd含量降低到《土壤环境质量农用地土壤污染风险管控标准(试行)》(GB 15618—2018)规定的风险筛选值(0.3 mg/kg)以下,可实现农用地的安全利用.
In order to investigate the remediation effect of the microbial-plant joint system on soil heavy metal Cd pollution, we carry out strain screening and pot experiment to explore the effects of plant growth-promoting bacteria on the accumulation capacity of ryegrass, soil physical and chemical properties and heavy metal speciation. The results show that the microscopic morphology of Burkholderia sp.YXL1 is pale yellow, rod-shaped and smooth edge with an average length of 5 μm, and it possesses strong growth-promoting ability and can tolerate cadmium content about 250 mg/L. The capacity of IAA production, siderophore and phosphate-solubilizing substances are 31.56 mg/L, 0.89 and 46.18 mg/L, respectively. In the YXL1-ryegrass joint remediation system, the organic matter and available phosphorus content in the soil are significantly increased by 4.08 g/kg and 2.54 mg/kg, respectively. The contents of weak acid extractable Cd and reducible Cd in the soil reach 16.25% and 12.53%, respectively. Compared with the control group, the ability of ryegrass to extract Cd from the soil is 2.76 times higher than that of the ryegrass treatment. The results also show that the Cd content of the cultivated layer soil is below the risk screening value (<0.3 mg/kg) in the Soil Environmental Quality Agricultural Land Soil Pollution Risk Control Standard (GB 15618—2018) to realize the safe use of agricultural land in the YXL1- ryegrass joint remediation system after 4 years.


[1] ZHAO Fangjie, MA Yibing, ZHU Yongguan, et al. Soil contamination in China: current status and mitigation strategies[J]. Environmental Science & Technology,2015, 49(2):750-780.
[2] KANG C H, KWON Y J, SO J S. Bioremediation of heavy metals by using bacterial mixtures[J]. Ecological Engineering, 2016, 89:64-69.
[3] 韩辉, 王晓宇, 蔡红,等.重金属固定植物促生细菌的筛选及其阻控小麦富集重金属效应[J].环境科学, 2019, 40(7): 3339-3346.
HAN Hui, WANG Xiaoyu, CAI Hong, et al. Isolation of heavy metal immobilizing and plant growth-promoting bacteria and its effects on reducing heavy metal accumulation in wheat[J]. Environmental Science, 2017, 40(7): 3339-3346.(in Chinese)
[4] WANG Liang, LIN Hai, DONG Yingbo, et al. Isolation of vanadium-resistance endophytic bacterium PRE01 from Pterisvittata in stone coal smelting district and characterization for potential use in phytoremediation[J].Journal of Hazardous Materials,2018,341:1-9.
[5] ESTRADA-BONILLA G, DURRER A, CARDOSO E J B N. Use of compost and phosphate-solubilizing bacteria affect sugarcane mineral nutrition, phosphorus availability, and the soil bacterial community[J]. Applied Soil Ecology, 2021,157:103760.
[6] MA Ying, OLIVEIRA R S, NAI Fengjiao, et al. The hyperaccumulator Sedum plumbizincicola harbors metal-resistant endophytic bacteria that improve its phytoextraction capacity in multi-metal contaminated soil[J]. Journal of Environmental Management, 2015, 156: 62-69.
[7] LIU Huakang, XIE Yanluo, LI Junjie, et al. Effect of Serratia sp. K3 combined with organic materials on cadmium migration in soil-vetiveriazizanioides L. system and bacterial community in contaminated soil[J]. Chemosphere, 2020, 242: 125164.
[8] 蒋淼,张丹,支月娥,等.强化龙葵富集镉根际促生菌的分离、筛选与鉴定[J].微生物学通报, 2019, 46(9): 2231-2240.
JIANG Miao, ZHANG Dan, ZHI Yue’e, et al. Isolation, screening and identification of plant growth promoting rhizobacteria to enrich cadmium accumulation in Solanumnigrum L.[J]. Microbiology China, 2019, 46(9): 2231-2240.(in Chinese)
[9] MA Ying, RAJKUMAR M, ZHANG Chang, et al. Inoculation of Brassica oxyrrhina with plant growth promoting bacteria for the improvement of heavy metal phytoremediation under drought conditions[J].Journal of Hazardous Materials, 2016, 320: 36-44.
[10] MA Ying, RAJKUMAR M, ZHANG Chang, et al. Beneficial role of bacterial endophytes in heavy metal phytoremediation[J]. Journal of Environmental Management, 2016, 174: 14-25.
[11] RAJKUMAR M, FREITAS H. Influence of metal resistant-plant growth-promoting bacteria on the growth of Ricinuscommunis in soil contaminated with heavy metals[J]. Chemosphere, 2008,71: 834-842.
[12] 鲍士旦.土壤农化分析[M].北京:中国农业出版社, 2000:86-115.
BAO Shidan. Analysis of soil and agrochemistry[M]. Beijing: China Agriculture Press,2000: 86-115.(in Chinese)
[13] QUEVAUVILLER P, RAURET G, GRIEPINK B. Single and sequential extraction in sediments and soils[J]. International Journal of Environmental Analytical Chemistry, 1993, 51(1/2/3/4):231-235.
[14] 贺玉龙,余江,谢世前,等.可生物降解螯合剂GLDA 强化三叶草修复镉污染土壤[J]. 环境科学,2020, 41(2):979-985.
HE Yulong, YU Jiang, XIE Shiqian, et al. Enhanced phytoextraction of cadmium contaminated soil by trifoliumrepens with biodegradable chelate GLDA[J]. Environmental Science, 2020, 41(2): 979-985.(in Chinese)
[15] SALAZAR J M, RODRIGUEZ H J, CID V C, et al. Auxin effects on Pb phytoextraction from polluted soils by Tegetesminuta L. and Bidenspilosa L.: extractive power of their root exudates[J].Journal of Hazardous Materials, 2016, Materials, 311:63-69.
[16] YIN Kun, WU Yixuan, WANG Shasha, et al. A sensitive fluorescent biosensor for the detection of copper ion inspired by biological recognition element pyoverdine[J]. Sensor and Actuators B Chemical, 2016, 232: 257-63.
[17] WANG Q, XIONG D, ZHAO P, et al. Effect of applying an arsenic-resistant and plant growth-promoting rhizobacterium to enhance soil arsenic phytoremediation by Populusdeltoides LH05-17[J]. Journal of Applied Microbiology, 2011, 111(5): 1065-1074.
[18] DIMKPA C O, MERTEN D, ALE S, et al. Metal-induced oxidative stress impacting plant growth in contaminated soil is alleviated by microbial siderophores[J]. Soil Biology and Biochemistry, 2009, 41: 154-62.
[19] KUMAR A, MAURYA B R, RAGHUWANSHI R. Isolation and characterization of PGPR and their effect on growth, yield and nutrient content in wheat (Triticumaestivum L.)[J].Biocatalysis and Agricultural Biotechnology, 2014, 3(4): 121-128.
[20] TRUYENS S, JAMBON I, CROES S, et al. The effect of longterm Cd and Ni exposure on seed endophytes of Agrostiscapillaris and their potential applicationin phytoremediation of metal-contaminated soils[J].International Journal Phytoremediation, 2014, 16: 643-659.
[21] TAGHAVI S, GARAFOLA C, MONCHY S, et al. Genome survey and characterization of endophytic bacteria exhibiting a beneficial effect on growth and development of poplar trees[J]. Applied and Environmental Microbiology, 2009, 75: 748-57.
[22] 郭晓方,卫泽斌, 许田芬,等. 不同pH值混合螯合剂对土壤重金属淋洗及植物提取的影响[J].农业工程学报, 2011, 27(7): 96-100.
GUO Xiaofang, WEI Zebin, XU Tianfen, et al. Effects of mixture of chelating agents with different pH values on phytoextraction and heavy metals removal[J]. Transactions of the CSAE, 2011, 27(7): 96-100.(in Chinese)
[23] 莫雪, 陈斐杰,游冲,等.黄河三角洲不同植物群落土壤酶活性特征及影响因子分析[J].环境科学, 2020, 41(2): 895-904.
MO Xue,CHENFeijie,YOU Chong, et al. Characteristics and factors of soil enzyme activity for different plant communities in Yellow River Delta[J]. Environmental Science,2020,41(2):895-904.(in Chinese)


 LIU Yi,LIN Xiao-dong,ZHANG Lei,et al.Study on preparation,structures and anticoagulation properties of diamond-like carbon films[J].Journal of Shenzhen University Science and Engineering,2008,25(4):287.
 YU Jiang and YANG Yu-feng.Response of seaweed Gracilaria lemaneiformis to combined toxicities of heavy metal and organic substance[J].Journal of Shenzhen University Science and Engineering,2009,26(4):343.
 ZHAO Yu-yan,LU Ji-long,HAO Li-bo,et al.Grey prediction of Cd,Hg pollution risk in Songhua Lake sediments[J].Journal of Shenzhen University Science and Engineering,2011,28(4):368.
 Ni Zhuo,Yang Sha,Wang Ying,et al.Research methods and advances on bone substitute materials[J].Journal of Shenzhen University Science and Engineering,2015,32(4):331.[doi:10.3724/SP.J.1249.2015.04331]
 Ni Zhuo,Yang Sha,Wu Gengfeng,et al.Thermal stability and biotoxicity of PES-HA biocomposites[J].Journal of Shenzhen University Science and Engineering,2017,34(4):313.[doi:10.3724/SP.J.1249.2017.03313]
 JIANG Yifeng,YANG Shili,WEI Wei,et al.Effect of nitrogen control on the coupling system of brewery waste water and microalgae cultivation[J].Journal of Shenzhen University Science and Engineering,2020,37(4):528.[doi:10.3724/SP.J.1249.2020.05528]
 YUAN Qiuhua,SHI Xin,LIANG Jinren,et al.Preparation and antibacterial properties of cerium-zinc co-doped hydroxyapatite-graphene composite[J].Journal of Shenzhen University Science and Engineering,2021,38(4):280.[doi:10.3724/SP.J.1249.2021.03280]


Foundation:National Key R & D Program of China(2018YFC1802605); Key Science and Technology Program of Sichuan Province(2017GZ0383,2017SZ0181); Strategic Cooperation Project of Sichuan University and Yibin City(2019CDYB-26)
Corresponding author:Professor YU Jiang. E-mail: yuj@scu.edu.cn
Citation:HE Huan, XIE Ruolan, DING Senxu, et al.Screening of cadmium resistant bacteria and their effects on plant growth promotion and remediation in soil[J]. Journal of Shenzhen University Science and Engineering, 2021, 38(3): 374-379.(in Chinese)
引文:何欢,谢若兰,丁森旭,等.重金属镉抗性菌的筛选及促生、修复效果[J]. 深圳大学学报理工版,2021,38(3):374-379.
更新日期/Last Update: 2021-07-30