[1]段军秀,郑嵋戈,穆淑花,等.tDCS对中风大鼠功能恢复和Notch信号通路的影响[J].深圳大学学报理工版,2020,37(5):521-527.[doi:10.3724/SP.J.1249.2020.05521]
 DUAN Junxiu,ZHENG Meige,et al.Effect of transcranial direct current stimulation on Notch pathway activation and functional recovery promotion in stroke rats[J].Journal of Shenzhen University Science and Engineering,2020,37(5):521-527.[doi:10.3724/SP.J.1249.2020.05521]
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tDCS对中风大鼠功能恢复和Notch信号通路的影响()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第37卷
期数:
2020年第5期
页码:
521-527
栏目:
生物工程
出版日期:
2020-09-15

文章信息/Info

Title:
Effect of transcranial direct current stimulation on Notch pathway activation and functional recovery promotion in stroke rats
文章编号:
202005010
作者:
段军秀12郑嵋戈3穆淑花4田大胜3许新忠3贺震旦1张健1
1)深圳大学基础医学院,广东深圳518060
2)明斯特大学生物物理及生物医学研究所,德国明斯特 48149
3)安徽医科大学第二附属医院骨科,安徽合肥230601
4)深圳大学心理学院,广东深圳518060
Author(s):
DUAN Junxiu1 2 ZHENG Meige3 MU Shuhua4 TIAN Dasheng3 XU Xinzhong3 HE Zhendan1 and ZHANG Jian1
1) School of Basic Medical Sciences, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China
2) Institute of Medical Physics and Biophysics, University of Münster, Münster 48149, Germany
3)Department of Orthopaedics, the Second Hospital of Anhui Medical University, Hefei 230601, Anhui Province, P.R.China;
4) College of Psychology, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China
关键词:
神经生物学中风经颅直流电刺激大脑中动脉阻塞Notch信号通路神经功能评分量表脑缺血
Keywords:
neurobiology stroke transcranial direct current stimulation middle cerebral arterial occlusion Notch pathway modified neurological severity score ischemic brains
分类号:
R743.3
DOI:
10.3724/SP.J.1249.2020.05521
文献标志码:
A
摘要:
临床研究表明,在大鼠双眼眶上孔、眶下缘和鼻交点处采用经颅直流电刺激(transcranial direct current stimulation, tDCS)有助于中风后的康复.然而,tDCS的潜在神经生物学机制仍然知之甚少.本研究主要关注卒中后tDCS疗法对功能恢复和Notch信号通路的影响.采用大鼠中脑动脉闭塞(middle cerebral arterial occlusion, MCAO)再灌注模型,将实验动物随机分为缺血组(MCAO)和缺血后治疗组(MCAO+tDCS),采用神经系统严重程度评分(modified neurological severity score, mNSS)结合动物的行为学表现对神经功能的恢复进行评估.通过2,3,5-三苯基氯化四氮唑(2,3,5-triphenyltetrazole chloride, TTC)染色从组织学上比较治疗前后大鼠脑缺血组织损伤的恢复情况.应用实时定量聚合酶链反应(real time quantitative polymerase chain reaction, RT-PCR)测定缺血性脑中Notch信号通路中受体蛋白、配体蛋白及下游因子的基因表达变化.结果表明,大鼠脑缺血可以激活Notch信号通路,采用多导联脑反射仪的tDCS疗法可以提高Notch信号通路中Notch1、NICD、RBP-JK和Hes-1 的mRNA水平,促进神经功能的恢复,并显著减少脑梗死的体积.多导联脑反射仪的tDCS疗法有望发展为一种可靠的临床治疗方式.
Abstract:
Clinical studies show that transcranial direct current stimulation (tDCS) in the intersections of the bilateral supraorbital foramen and inferior orbital rim and nose is helpful for rehabilitation after stroke. However, the neurobiological mechanism of tDCS is poorly understood. Here we focus on the effect of tDCS on functional recovery and Notch signaling pathway after stroke. Middle cerebral arterial occlusion (MCAO) reperfusion is induced in rats. Animals with comparable infarcts are randomly divided into ischemia group (MCAO) and post-ischemia treatment group (MCAO + tDCS). Neural system severity score (mNSS) combined with animal behavioral performance is used to evaluate the recovery of neural function. TTC (2,3,5-triphenyltetrazolium chloride) staining is applied to compare histologically the recovery of ischemic tissue injury before and after treatments. Real-time quantitative polymerase chain reaction (RT-PCR) is used to determine mRNA expression changes of receptor protein, ligand protein, and downstream factor in the Notch signaling pathway in ischemic brains. The results reveal that ischemia could activate Notch signaling pathway, tDCS treated by multilead brain reflex instrument could facilitate mRNA expression of Notch1, NICD, RBP-JK and Hes-1, promote the recovery of neurological function, and significantly reduce the volume of cerebral infarct. Our study suggests that tDCS with multi-lead brain reflex instrument can be expected to become a reliable clinical treatment.

参考文献/References:

[1] ZHAO Jin, GUO Pei, LI Xinyi,et al.Neuroprotective effects of irisin against cerebral ischemia/reperfusion injury via Notch signaling pathway[J].Biomedicine & Pharmacotherapy,2019,120:109452.
[2] ARUMUGAM T V, SANG-HA B, PRIYANKA B,et al.Notch signaling and neuronal death in stroke[J].Progress in Neurobiology,2018,165/166/167(167):103-116.
[3] ZHANG Runrui, ENGLER A, VERDON T.Notch: an interactive player in neurogenesis and disease[J].Cell and Tissue Research,2018,371(1):73-89.
[4] NCHEZ-KUHN A, PREZ-FERNNDEZ C, CNOVAS R,et al.Transcranial direct current stimulation as a motor neurorehabilitation tool: an empirical review[J].Biomedical Engineering Online,2017,16(Suppl. 1):76.
[5] RAN Yuan, LIU Zongjian, SHUO Huang,et al.Splenectomy fails to provide long-term protection against ischemic stroke[J].Aging and Disease,2018,9(3):467-479.
[6] DANIEL C M, WING L C, RICHARD L,et al.Thymosin β4 for the treatment of acute stroke in aged rats[J].Neuroscience Letters,2017,659: 7-13.
[7] SWANSON R A, MORTON M T, TSAO-WU G,et al.A semiautomated method for measuring brain infarct volume[J]. Journal of Cerebral Blood Flow and Metabolism,1990,10(2):290-293.
[8] SATURNINO G B, ANTUNES A, THIELSCHER A.On the importance of electrode parameters for shaping electric field patterns generated by tDCS[J].NeuroImage,2015,120: 25-35.
[9] WOODS A J, BRYANT V, SACCHETTI D,et al.Effects of electrode drift in transcranial direct current stimulation[J].Brain Stimulation,2015,8(3):515-519.
[10] FLEMING M K, THEOLOGIS T, BUCKINGHAM R,et al.Transcranial direct current stimulation for promoting motor function in cerebral palsy: a review[J].Journal of NeuroEngineering and Rehabilitation,2018,15(1): 121.
[11] LADENBAUER J, LADENBAUER J, KLZOW N,et al.Promoting sleep oscillations and their functional coupling by transcranial stimulation enhances memory consolidation in mild cognitive impairment[J].The Journal of Neuroscience,2017,37(30):7111-7124.
[12] ANDROUTSELLIS-THEOTOKIS A, LEKER R R, SOLDNER F,et al.Notch signalling regulates stem cell numbers in vitro and in vivo[J].Nature,2006,442(714):823-826.
[13] SUN Fen, MAO Xiaoou, XIE Lin,et al.Notch1 signaling modulates neuronal progenitor activity in the subventricular zone in response to aging and focal ischemia[J].Aging Cell,2013,12(6):978-987.
[14] WANG Xiaomei, MAO Xiaoou, XIE Lin,et al.Involvement of notch1 signaling in neurogenesis in the subventricular zone of normal and ischemic rat brain in vivo[J].Journal of Cerebral Blood Flow and Metabolism,2009,29(10):1644-1654.
[15] FELLING R J, LEVISON S W.Enhanced neurogenesis following stroke[J].Journal of Neuroscience Research,2003,73(3):277-283.
[16] DHANESH S B, SUBASHINI C, JAMES J. Hes1: the maestro in neurogenesis[J].Cellular and Molecular Life Sciences,2016,73(21):4019-4042.
[17] WANG Lei, CHOPP M, ZHANG Ruilan, et al.The Notch pathway mediates expansion of a progenitor pool and neuronal differentiation in adult neural progenitor cells after stroke[J].Neuroscience,2009,158(4): 1356-1363.

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备注/Memo

备注/Memo:
Received:2020-02-13;Accepted:2020-03-28
Foundation:National Natural Science Foundation of China(81400064)
Corresponding author:Professor ZHANG Jian. E-mail: jzhanghappy@163.com
Citation:DUAN Junxiu, ZHENG Meige, MU Shuhua, et al. Effect of transcranial direct current stimulation on Notch pathway activation and functional recovery promotion in stroke rats[J]. Journal of Shenzhen University Science and Engineering, 2020, 37(5): 521-527.(in Chinese)
基金项目:国家自然科学基金资助项目(81400064)
作者简介:段军秀(1990—),明斯特大学博士研究生.研究方向:突触前膜蛋白组成及结构. E-mail: jxduan_anne@163.com
引文:段军秀, 郑嵋戈, 穆淑花, 等. tDCS对中风大鼠功能恢复和Notch信号通路的影响[J]. 深圳大学学报理工版,2020,37(5):521-527.
更新日期/Last Update: 2020-07-26