[1]李琳,罗淋淋,罗光宇,等.一种植物基因组DNA快速提取方法的建立与评估[J].深圳大学学报理工版,2020,37(1):1-8.[doi:10.3724/SP.J.1249.2020.01001]
 LI Lin,LUO Linlin,et al.Establishment and evaluation of a method for rapid extraction of plant genomic DNA[J].Journal of Shenzhen University Science and Engineering,2020,37(1):1-8.[doi:10.3724/SP.J.1249.2020.01001]
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一种植物基因组DNA快速提取方法的建立与评估()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第37卷
期数:
2020年第1期
页码:
1-8
栏目:
生物工程
出版日期:
2020-01-08

文章信息/Info

Title:
Establishment and evaluation of a method for rapid extraction of plant genomic DNA
文章编号:
202001001
作者:
李琳12罗淋淋123罗光宇123莫蓓莘12刘琳12
1)深圳大学生命与海洋科学学院,广东省植物表观遗传学重点实验室,广东深圳518060
2)深圳大学龙华生物产业创新研究院,广东深圳518060
3)深圳大学物理与光电工程学院,广东深圳518060
Author(s):
LI Lin1 2 LUO Linlin1 2 3 LUO Guangyu1 2 3 MO Beixin1 2 and LIU Lin1 2
1) College of Life Sciences and Oceanography, Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China
2) Longhua Bioindustry and Innovation Research Institute, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China
3) College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China
关键词:
分子生物学基因组脱氧核糖核酸快速提取聚合酶链式反应水稻拟南芥基因型鉴定单核苷酸多态性分析简单重复序列标记分析
Keywords:
molecular biology genomic deoxyribonucleic acid rapid extraction polymerase chain reaction Oryza sativa L. Arabidopsis thaliana genotyping single nucleotide polymorphisms analysis simple sequence repeat analysis
分类号:
Q947.1
DOI:
10.3724/SP.J.1249.2020.01001
文献标志码:
A
摘要:
植物基因组脱氧核糖核酸(deoxyribonucleic acid, DNA)的提取是植物分子实验的基础,但随着样本数量的增加,基因组DNA的提取成为植株基因型鉴定以及单核苷酸多态性检测等分子实验的限速环节.为解决现有技术中提取植物基因组DNA过程繁琐、耗时耗力的问题,从提取液配方和提取条件等方面改良常规快速植物基因组DNA提取法,缩短了样本制备时间并简化了提取步骤,改良后每个样本DNA制备需时不到90 s,同时采用96孔板和配套设施显著提高了实验通量.该方法对植物样本的状态要求低、扩增效率高、成本低,适用于拟南芥、水稻和玉米等多种植物基因组DNA的提取与聚合酶链式反应(polymerase chain reaction, PCR)扩增实验,尤其适用于大样本量的规模化基因型鉴定.
Abstract:
The extraction of plant genomic deoxyribonucleic acid (DNA) is the basis for plant molecular experiments. However, as the number of plant samples increases, genomic DNA extraction becomes the rate-limiting step in molecular experiments such as the identification of plant genotypes and single nucleotide polymorphisms detection. Regular DNA extraction is usually complicated and time-consuming and effort-consuming. To cope with these problems, we improve the conventional rapid plant genomic DNA extraction method from the aspects of extraction buffer and extraction conditions to further shorten the sample preparation time and simplify the extraction steps. After optimization, the preparation of each DNA sample takes less than 90 s, and adoption of 96 wells plates and related facilities significantly improve experimental throughput. This system has low requirements for the status of plant samples, and has high amplification efficiency at low cost. Besides, this system is suitable for a variety of plant species such as Arabidopsis thaliana L., rice (Oryza sativa L.), and maize (Zea mays L.) for genomic DNA extraction and polymerase chain reaction (PCR) amplification, especially for genotyping experiments that require a large number of samples.

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备注/Memo

备注/Memo:
Received:2018-11-01;Revised:2019-05-17;Accepted:2019-06-05
Foundation:National Key Research and Development Program of China (2016YFD0101803);National Natural Science Foundation of China (31600982); Guangdong Innovation Research Team Fund (2014ZT05S078); Shenzhen High-level Talents Research Fund (827/000256); Shenzhen University Research Fund (2016095)
Corresponding author:Assistant professor LIU Lin. E-mail: linliu@szu.edu.cn
Citation:LI Lin, LUO Linlin, LUO Guangyu, et al. Establishment and evaluation of a method for rapid extraction of plant genomic DNA[J]. Journal of Shenzhen University Science and Engineering, 2020, 37(1): 1-8.(in Chinese)
基金项目:国家重点研发计划资助项目(2016YFD0101803);国家自然科学基金资助项目(31600982);广东省创新创业团队资助项目(2014ZT05S078);深圳市高端人才科研基金资助项目(827/000256);深圳大学科研基金资助项目(2016095)
作者简介:李琳(1994—),深圳大学硕士研究生.研究方向:植物表观遗传学.E-mail:15513820966@163.com
引文:李琳,罗淋淋,罗光宇,等.一种植物基因组DNA快速提取方法的建立与评估[J]. 深圳大学学报理工版,2020,37(1):1-8.
更新日期/Last Update: 2020-01-30