[1]张尉欣,刘阳,刘 帅,等.镉胁迫下菜心的转录组分析[J].深圳大学学报理工版,2018,35(5):543-550.[doi:10.3724/SP.J.1249.2018.05543]
　ZHANG Weixin,LIU Yang,LIU Shuai,et al.Transcriptome analysis of Brassica rapa ssp. chinensis var. parachinensis under cadmium stress[J].Journal of Shenzhen University Science and Engineering,2018,35(5):543-550.[doi:10.3724/SP.J.1249.2018.05543]
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镉胁迫下菜心的转录组分析()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第35卷
期数:
2018年第5期
页码:
543-550
栏目:
生物工程
出版日期:
2018-09-25

文章信息/Info

Title:
Transcriptome analysis of Brassica rapa ssp. chinensis var. parachinensis under cadmium stress
作者:
张尉欣1; 刘阳1; 刘 帅1; 唐玉林1; 2
1）深圳大学生命与海洋科学学院，广东省植物表观遗传学重点实验室，广东深圳 518060；2）深圳市海洋生物资源与生态环境重点实验室，广东深圳 518060
Author(s):
ZHANG Weixin1;  LIU Yang1;  LIU Shuai1;  and TANG Yulin1; 2
1） Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China 2) Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Sciences, Shenzhen 518060, Guangdong Province, P.R.China
关键词:
植物遗传学; 生物信息学; 高通量测序; 转录组; 镉胁迫; 菜心
Keywords:
plant genetics;  bioinformatics;  high-throughput sequencing;  transcriptome;  cadmium stress;  Brassica rapa ssp.chinensis var.Parachinensis
分类号:
Q 943.2；Q 786
DOI:
10.3724/SP.J.1249.2018.05543
文献标志码:
A
摘要:
为研究菜心吸收和转运镉的机制，对镉胁迫前后菜心叶部和根部的转录组进行高通量测序及差异表达分析，共获得3 854个差异表达基因．GO（gene ontology）富集分析显示，差异表达基因主要富集在与次生代谢物合成、氧化还原反应、光合作用、铁离子稳态及木质素或木栓质的合成及代谢等有关的GO条目中．KEGG（kyoto encyclopedia of genes and genomes）富集分析结果表明，差异表达上调基因主要富集在植物病原菌互作、植物信号传导、木栓质等物质的生物合成和谷胱甘肽代谢等通路中；差异表达下调基因主要富集在光合作用、核糖体、生物素代谢、脂肪酸的生物合成和芥子油苷的生物合成等通路中；这些差异表达基因可能与菜心应对镉胁迫的生理生化反应相关．分析了菜心在镉胁迫下响应的主要通路及基因，为进一步探索菜心镉胁迫响应机制、培养低镉积累的菜心品种提供理论基础．
Abstract:
In order to investigate the mechanism of absorption and transportation of cadmium in Choy-sum (Brassica rapa ssp.chinensis var. parachinensis), and cultivate low accumulation of cadmium（Cd） varieties, by comparing the transcriptome of the shoots and roots of Choy-sum before and after cadmium stress, total 3 854 differentially expressed genes (DEGs) were annotated. The gene ontology (GO) enrichment analysis showed that most of the DEGs are predominantly enriched in the terms of those related to secondary metabolic process, photosynthesis, redox reaction, ion transport, suberin biosynthesis process, lignin catabolic process and so on. The KEGG enrichment analysis showed that the down-regulated DEGs are predominantly enriched in the pathways involved in photosynthesis, ribosome, biotin metabolism, fatty acid biosynthesis and glucosinolate biosynthesis. The up-regulated DEGs are predominantly enriched in the pathways involved in plant-pathogen interaction, plant signal transduction, suberine biosynthesis, glutathione metabolism and so on. The DEGs might be related to plant response to cadmium stress. The study reveals the genes and pathways involved in response to Cd stress of Choy-sum and extends our understanding of the function of plant in response to Cd stress. It will also provide theoretical basis and novel strategies for creating new varieties of choy-sum with low accumulation of Cd via molecular breeding methods.

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更新日期/Last Update: 2018-08-21