[1]陆佳运,宋剑波,王晓彦,等.核苷酸转移酶与玉米非生物胁迫响应[J].深圳大学学报理工版,2018,35(5):529-535.[doi:10.3724/SP.J.1249.2018.05529]
 LU Jiayun,SONG Jianbo,WANG Xiaoyan,et al.Nucleotidyl transferase proteins and the biological responses of Zea mays to abiotic stress[J].Journal of Shenzhen University Science and Engineering,2018,35(5):529-535.[doi:10.3724/SP.J.1249.2018.05529]
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核苷酸转移酶与玉米非生物胁迫响应()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第35卷
期数:
2018年第5期
页码:
529-535
栏目:
生物工程
出版日期:
2018-09-25

文章信息/Info

Title:
Nucleotidyl transferase proteins and the biological responses of Zea mays to abiotic stress
作者:
陆佳运1宋剑波12王晓彦1莫小为1王碎抗1许欣彤1劳康文1莫蓓莘1
1)深圳大学生命与海洋科学学院,广东省表观遗传学重点实验室,广东深圳518060;2)江西农业大学理学院,江西南昌330045
Author(s):
LU Jiayun1 SONG Jianbo12 WANG Xiaoyan1 MO Xiaowei1 WANG Suikang1 XU Xintong1 LAO Kangwen1 and MO Beixin1
1) Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life and Oceanography, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China 2) College of Science, Jiangxi Agricultural University, Nanchang 330045, Jiangxi Province,P.R.China
关键词:
核苷酸转移酶玉米拟南芥水稻顺式作用元件反转录聚合酶链式反应非生物胁迫
Keywords:
nucleotidyl transferase protein Zea mays Arabidopisis thaliana Oryza sativa cis-acting elements realtime fluorescence quantitative polymerase chain reaction (RT-PCR) abiotic stress
分类号:
Q 946.1
DOI:
10.3724/SP.J.1249.2018.05529
文献标志码:
A
摘要:
核苷酸转移酶(nucleotidyl transferase protein,NTP)能够对RNA 3′末端进行修饰,从而影响其稳定性.通过分析玉米NTP蛋白家族的结构域与拟南芥、水稻中NTP蛋白进化的关系,发现玉米中含有24种NTP基因,并且玉米、拟南芥和水稻3种植物中的NTP基因具有高度同源性与保守性.对玉米NTP基因启动子上游顺式作用元件进行预测分析,结果显示,玉米NTP基因启动子中含有多个与非生物胁迫相关的启动子元件.通过反转录聚合酶链式反应分析在高盐、干旱、植物激素朊落酸的非生物胁迫条件下,玉米NTP相关基因的表达量,研究发现,玉米NTP基因能被非生物胁迫诱导表达.实验表明玉米NTP基因与非生物胁迫相关,且可能参与了植株抵抗逆境胁迫的过程.
Abstract:
Nucleotidyl transferase proteins (NTPs) can modify the 3′ ends of small RNA and mRNA and affect their stability. By analyzing the domain and evolutionary relationship of NTPs in Zea mays, Arabidopisis thaliana and Oryza sativa, we find that there are 24 NTPs in Zea mays, and the NTPs in Zea mays, Arabidopsis thaliana, and Oryza sativa are highly homologous and conserved. Sequence analysis shows that cis-acting elements of Zea mays’ NTP gene promoters contain several elements that are related to abiotic stress responses. Further analysis of realtime fluorescence quantitative polymerase chain reaction (RT-PCR) data shows that the expressions of NTP genes are induced under high salinity, drought, and abscisic acid stress, which indicates that NTPs may be involved in plant stress resistance pathway.
更新日期/Last Update: 2018-08-21