[1]郭志凯,盖翠娟,蔡彩虹,等.Quinadoline C, 海南海桑内生真菌的一个新生物碱[J].深圳大学学报理工版,2018,35(4):334-338.[doi:10.3724/SP.J.1249.2018.04334]
　GUO Zhikai,GAI Cuijuan,CAI Caihong,et al.Quinadoline C, a new alkaloid from mangrove Sonneratia hainanensis-associated Aspergillus sp.[J].Journal of Shenzhen University Science and Engineering,2018,35(4):334-338.[doi:10.3724/SP.J.1249.2018.04334]
点击复制

Quinadoline C, 海南海桑内生真菌的一个新生物碱()
分享到：

《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第35卷
期数:
2018年第4期
页码:
334-338
栏目:
生物工程
出版日期:
2018-07-10

文章信息/Info

Title:
Quinadoline C, a new alkaloid from mangrove Sonneratia hainanensis-associated Aspergillus sp.
作者:
郭志凯; 盖翠娟; 蔡彩虹; 曾艳波; 袁靖喆; 梅文莉; 戴好富
中国热带农业科学院热带生物技术研究所；农业部热带作物生物学与遗传资源利用重点实验室，海南海口571101
Author(s):
GUO Zhikai;  GAI Cuijuan;  CAI Caihong;  ZENG Yanbo;  YUAN Jingzhe;  MEI Wenli;  and DAI Haofu
Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture; Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan Province, P.R.China
关键词:
红树植物; 海南海桑; 内生真菌; 曲霉菌; 天然产物; 新吲哚生物碱
Keywords:
Mangrove;  Sonneratia hainanensis;  endophytic fungi;  Aspergillus sp.;  natural products;  new indole alkaloid
分类号:
Q 939.92；R 978.1
DOI:
10.3724/SP.J.1249.2018.04334
文献标志码:
A
摘要:
红树植物内生真菌是寻找结构新颖天然产物的重要来源，已引起天然产物化学研究者的广泛重视. 采用正相、反相硅胶柱色谱和Sephadex LH20凝胶柱色谱法，从海南特有红树植物海南海桑内生曲霉菌菌株Aspergillus sp. HS02的麦芽培养基发酵液乙酸乙酯提取物中，分离得到了化合物1，该化合物为发酵液主要成分，利用高分辨电喷雾质谱、一维核磁共振波谱、异核单量子相关谱、异核多键相关谱和氢-氢质子同核相关谱对化合物1结构进行了鉴定，确定为一个新的吲哚生物碱，命名为quinadoline C. 利用滤纸片琼脂扩散法评价了该化合物抗农业病原真菌活性.
Abstract:
Mangrove-derived endophytic fungi are important and promising resources for the novel natural products discovery and have attracted much attention of natural product chemists. A strain of fungus Aspergillus sp. HS02 was isolated from the leaf of the mangrove plant Sonneratia hainanensis endemic in Hainan Island. Fermentation of this fungus was conducted in liquid malt extract medium (MEA). From the ethyl acetate extract of the fermentation broth, one major component 1 was isolated and purified by normal phase, reversed-phase silica gel and sephadex LH20 column chromatography. The structure of compound 1 was identified as a new indole alkaloid by high resolution electrospray ionization mass spectroscopy, one-dimensional nuclear magnetic resonance spectroscopy, heteronuclear single quantum correlation, heteronuclear multiple bond correlation and 1H-1H correlated spectroscopy, and names as quinadoline C. The antifungi activity of 1 against two agricultural pathogenic fungi was evaluated in vitro by filter paper disc diffusion method.
更新日期/Last Update: 2018-06-20