[1]郭志凯,盖翠娟,蔡彩虹,等.土壤链霉菌Streptomyces sp.的抑菌成分[J].深圳大学学报理工版,2018,35(2):166-171.[doi:10.3724/SP.J.1249.2018.02166]
 GUO Zhikai,GAI Cuijuan,CAI Caihong,et al.Antibacterial metabolites from the soil-derived actinobacteria Streptomyces sp.[J].Journal of Shenzhen University Science and Engineering,2018,35(2):166-171.[doi:10.3724/SP.J.1249.2018.02166]

土壤链霉菌Streptomyces sp.的抑菌成分()




Antibacterial metabolites from the soil-derived actinobacteria Streptomyces sp.
GUO Zhikai GAI Cuijuan CAI Caihong CHEN Liangliang DAI Haofu and MEI Wenli
Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan Province, P.R.China
bioactive natural products actinobacteria Streptomyces sp. column chromatography secondary metabolites antibacterial activity
Q 939.92;R 978.1
为从放线菌次生代谢产物中寻找结构新颖的活性先导分子,通过抗菌活性筛选,发现一株土壤链霉菌Streptomyces sp.的发酵提取物具有抑菌活性. 对该菌进行大规模发酵,利用正相、反相硅胶柱层析和半制备高效液相色谱法,从其发酵产物中分离得到化合物1—11,利用高分辨质谱、一维和二维核磁共振技术,分别将其结构鉴定为(E)-3-甲硫基丙烯酸(1)、(E)-3-甲硫基丙烯酰胺(2)、反式肉桂酸(3)、(E)-4-苯基-3-丁烯酸(4)、 3-羟基-4-甲氧基肉桂酰胺(5)、 3-indoleketol(6)、 N-乙酰基-β-氧色胺(7)、 2-乙酰基大黄素(8)、7-羟基-2-(2-羟丙基)-5-甲基色酮(9)、环(苯丙-脯)(10)和环(3-羟基酪-脯)(11). 其中,化合物4—6、8、9和11是首次从链霉菌中发现,并且首次报道了化合物2的 13C核磁数据. 体外对化合物2—4、6、8和9进行了抗菌活性测试,结果表明,化合物3和6对枯草芽孢杆菌和耻垢分支杆菌具有弱抑菌活性,化合物8对枯草芽孢杆菌、金黄色葡萄球菌和耻垢分支杆菌也具有弱抑菌活性.
Aiming to find bioactive natural products with novel structures from the secondary metabolites of actinomycetes and through screening the in vitro antibacterial activity, we find that the fermentation broth of the soil-derived Streptomyces sp. shows inhibitory activity against several bacteria. The strain is then fermented in a large scale. Eleven compounds (1-11) from the ethyl acetate extract are isolated by using various column chromatography. Their structures are (E)-3-(methylthio)acrylic acid (1), (E)-3-(methylthio)acrylamide (2), (E)-cinnamic acid (3), (E)-4-phenyl-3-butenoic acid (4), 3-hydroxy-4-methoxycinnamamide (5), 3-indoleketol (6), N-acetyl-β-oxotryptamine (7), 2-acetylemodin (8), 7-hydroxy-2-(2-hydroxypropyl)-5-methylchromone (9), cyclo (Phe-Pro) (10), and (3S-cis)-hexahydro-3-[(3,4-dihydroxyphenyl)methyl]pyrrolo[1,2-a]pyrazine-1,4-dione (11), which are identified by HRESIMS, 1D and 2D NMR analysis, and comparison of their NMR data with those published previously. Compounds 4-6, 8, 9 and 11 are firstly discovered from Streptomyces and the 13C NMR data of compound 2 is also firstly reported. Compounds 2-4, 6, 8 and 9 are assessed for in vitro antibacterial activity. Results show that the compounds 3 and 6 display weak activity against the gram-positive Bacillus subtilis and Mycobacterium smegmatis, while compound 8 exhibits weak activity against Bacillus subtilis and Mycobacterium smegmatis and Staphylococcus aureus.


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Foundation:National Natural Science Fundation of China (41506096, 41776093, 41406083); Natural Science Foundation of Hainan Province(20163117, 217254)
Corresponding author:Associate professor GUO Zhikai.E-mail: guozhikai@itbb.org.cn
Citation:GUO Zhikai,GAI Cuijuan,CAI Caihong,et al.Antibacterial metabolites from the soil-derived actinobacteria Streptomyces sp.[J]. Journal of Shenzhen University Science and Engineering, 2018, 35(2): 166-171.(in Chinese)
基金项目:国家自然科学基金资助项目(41506096, 41776093, 41406083) ;海南省自然科学基金资助项目(20163117, 217254)
引文:郭志凯,盖翠娟,蔡彩虹,等.土壤链霉菌Streptomyces sp.的抑菌成分研究[J]. 深圳大学学报理工版,2018,35(2):166-171.
更新日期/Last Update: 2018-03-07