[1]曹会,蔡德丰,杨平常,等.家蚕MBP-Bmlp7蛋白的克隆表达及免疫学鉴定[J].深圳大学学报理工版,2017,34(2):117-121.
 Cao Hui,Cai Defeng,Yang Pingchang,et al. Expression and characterization of allergenicity of MBP-Bmlp7 from Bombyx mori[J].Journal of Shenzhen University Science and Engineering,2017,34(2):117-121.
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家蚕MBP-Bmlp7蛋白的克隆表达及免疫学鉴定()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第34卷
期数:
2017年第2期
页码:
117-121
栏目:
生物工程
出版日期:
2017-03-20

文章信息/Info

Title:
 Expression and characterization of allergenicity of MBP-Bmlp7 from Bombyx mori
作者:
 曹会1蔡德丰2杨平常1刘志刚1夏立新1
 1)深圳大学医学部,呼吸疾病国家重点实验室深圳大学变态反应分室,广东深圳518060;2)深圳市儿童医院检验科,广东深圳 518026
Author(s):
 Cao Hui1 Cai Defeng2 Yang Pingchang1 Liu Zhigang1 and Xia Lixin1  
 1)Health Science Center, State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China; 2) Department of Clinical Laboratory, Shenzhen Children’s Hospital, Shenzhen 518026, Guangdong Province, P.R.China
关键词:
 免疫学家蚕30 kD脂蛋白表达纯化免疫印迹融合蛋白免疫原性
Keywords:
 immunology Bombyx mori 30 kD lipoprotein expression and purification immunoblotting fusion protein allergenicity
分类号:
R 392.8;Q 966
文献标志码:
A
摘要:
克隆表达家蚕蚕蛹30 u(1 u=1 kD)脂蛋白家族成员Bmlp7(Bombyx mori lipoprotein 7)的融合蛋白,鉴定其致敏原性.合成Bmlp7的基因后连接至载体质粒pMAL-C5e上,在大肠杆菌BL21中诱导表达出麦芽糖结合蛋白(maltose binding protein, MBP)-Bmlp7,纯化后,利用western blot鉴定MBP-Bmlp7融合蛋白与家蚕过敏患者血清中特异性免疫球蛋白E(immunoglobulins E, IgE)的结合能力.结果显示,纯化得到了纯度较高的MBP-Bmlp7融合蛋白,且该融合蛋白能够与家蚕过敏患者血清特异性IgE结合,说明Bmlp7是家蚕中一种潜在的致敏原.
Abstract:
 The open reading frame of Bmlp7 from silkworm (Bombyx mori) was chemically synthesized and cloned into plasmid pMAL-c5e. The recombinant plasmid pMAL-Bmlp7 was transformed into E.coli BL21 and expressed as a fusion protein. The protein was purified using Amylose affinity chromatography and purified to homogeneity. The IgE reactivity of the fusion protein was assayed by western blotting using sera of patient with silkworm allergies. The results show that the Bmlp7 fusion protein had IgE reactivity with the patient sera.

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更新日期/Last Update: 2017-03-03