[1]黎丽,李玉珍,丁利平,等.角蒿咖啡酸酯对小鼠耳肿胀的抑制作用[J].深圳大学学报理工版,2017,34(1):46-50.[doi:10.3724/SP.J.1249.2017.01046]
 Li Li,Li Yuzhen,et al.Effect of Incarvillea caffeate on mouse ear edema[J].Journal of Shenzhen University Science and Engineering,2017,34(1):46-50.[doi:10.3724/SP.J.1249.2017.01046]
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角蒿咖啡酸酯对小鼠耳肿胀的抑制作用()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第34卷
期数:
2017年第1期
页码:
46-50
栏目:
生物工程
出版日期:
2017-01-09

文章信息/Info

Title:
Effect of Incarvillea caffeate on mouse ear edema
文章编号:
201701007
作者:
黎丽12李玉珍2丁利平1黄钟1
1) 深圳大学医学部,广东深圳 518060
2) 中山大学第八附属医院药学部,广东深圳518033
Author(s):
Li Li1 2 Li Yuzhen2 Ding Liping1 and Huang Zhong1
1) School of Medicine, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China
2) Department of Pharmacy, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen 518033, Guangdong Province, P.R.China
关键词:
药理学耳肿胀角蒿咖啡酸酯花生四烯酸5-脂氧酶白三烯B4
Keywords:
pharmacology ear edema Incarvillea caffeate arachidonic acid (AA) 5-lipoxygenase (5-LOX) leukotrienes B4 (LTB4)
分类号:
R 392
DOI:
10.3724/SP.J.1249.2017.01046
文献标志码:
A
摘要:
研究了角蒿咖啡酸酯((+)-2-(1-hydroxyl-4-oxocyclohexyl) ethyl caffeate, JH2)对花生四烯酸(arachidonic acid, AA)致小鼠耳肿胀的抑制作用,探讨其作用机制,采用花生四烯酸诱导的小鼠急性耳肿胀模型评价JH2的体内药效,通过酶联免疫吸附测定实验检测JH2对AA通路中5-脂氧合酶(5-lipoxygenase, 5-LOX)活性的影响.实验结果显示,在AA诱导的小鼠耳肿胀模型中,JH2能显著抑制AA所致的小鼠耳肿胀程度和组织蛋白渗出量,并降低小鼠发炎耳组织中白三烯B4 (leukotrienes B4, LTB4) 的生成量.体外活性研究显示,JH2对5-LOX有较强的抑制活性,半抑制浓度IC50值为12.16 μmol/L.说明JH2对AA诱导的小鼠耳肿胀急性炎症具有抑制作用,其作用机制可能与JH2抑制AA通路中5-LOX的活性从而降低炎症介质LTB4的产生有关.
Abstract:
We investigated the effect of Incarvillea caffeate ((+)-2-(1-hydroxyl-4-oxocyclohexyl) ethyl caffeate, JH2) on arachidonic acid (AA) and its possible mechanism. We used AA-induced mouse acute ear edema model to determine the effect of JH2 on in vivo. We also studied the effect of JH2 on the enzyme activities of 5-lipoxygenase (5-LOX) by ELISA. The results showed that topical treatment with JH2 markedly inhibits the extent of oedema and protein permeability. Importantly, leukotrienes B4 (LTB4) production in ear mouse is also drastically suppressed by JH2 and reaches a maximum inhibition rate of 87.50%. Activity assay in vitro showed that JH2 inhibits 5-LOX activity with half maximal inhibitory concentration (IC50) of 12.16 μmol/L. JH2 presents anti-inflammatory effect on AA-induced mouse acute ear edema model through controlling the excessive production of LTB4 by inhibiting the activity of 5-LOX.

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备注/Memo

备注/Memo:
Received:2016-07-07;Accepted:2016-07-12
Foundation:Postdoctoral Science Foundation of China (2015M572370)
Corresponding author:Chief physician Li Yuzhen. E-mail: 695768596@qq.com;
Postdoctoral Ding Liping. E-mail: 13552432187@163.com
Citation:Li Li, Li Yuzhen, Ding Liping, et al. Effect of Incarvillea caffeate on mouse ear edema[J]. Journal of Shenzhen University Science and Engineering, 2017, 34(1): 46-50.(in Chinese)
基金项目:中国博士后科学基金资助项目(2015M572370)
作者简介:黎丽 (1986—),女,深圳大学博士后研究人员. 研究方向:天然产物机制研究.E-mail: wlili19860318123@163.com
引文:黎丽,李玉珍,丁利平,等. 角蒿咖啡酸酯对小鼠耳肿胀的抑制作用[J]. 深圳大学学报理工版,2017,34(1):46-50.
更新日期/Last Update: 2016-12-30