[1]马轩,李盛本,莫蓓莘,等.拟南芥ago1-27突变体的RNA-seq分析[J].深圳大学学报理工版,2017,34(1):27-32.[doi:10.3724/SP.J.1249.2017.01027]
　Ma Xuan,Li Shengben,Mo Beixin,et al.RNA-seq analysis on Arabidopsis ago1-27 mutant[J].Journal of Shenzhen University Science and Engineering,2017,34(1):27-32.[doi:10.3724/SP.J.1249.2017.01027]
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拟南芥ago1-27突变体的RNA-seq分析()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第34卷
期数:
2017年第1期
页码:
27-32
栏目:
生物工程
出版日期:
2017-01-09

文章信息/Info

Title:
RNA-seq analysis on Arabidopsis ago1-27 mutant
文章编号:
201701005
作者:
马轩1; 2; 李盛本3; 莫蓓莘1; 曹晓风2; 陈雪梅1; 4
1）深圳大学生命与海洋科学学院，广东深圳518060；2）中国科学院遗传与发育生物学研究所，北京100101；
3）中国农业科学院深圳农业基因组研究所，广东深圳518120；4）美国加利福尼亚大学河滨分校植物科学系, 加利福尼亚河滨CA 92521，美国
Author(s):
Ma Xuan1; 2;  Li Shengben3;  Mo Beixin1;  Cao Xiaofeng2;  and Chen Xuemei1; 4
1）College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China
2）Institute of Genetics and Developmental Biology, Chinese Academy of Sciences,Beijing 100101, P.R.China
3）Shenzhen Institute of Agricultural Genomics, Chinese Academy of Agricultural Sciences,Shenzhen 518120, Guangdong Province, P.R.China
4）Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, California 92521, USA
关键词:
植物生物化学; miRNA; ago1-27基因; RNA-seq; 降解组; 靶基因; 拟南芥
Keywords:
phytobiochemistry;  microribonucleic acid (miRNA);  ago1-27;  RNA-seq;  degradome;  target genes;  Arabidopsis
分类号:
Q 946.2
DOI:
10.3724/SP.J.1249.2017.01027
文献标志码:
A
摘要:
miRNA在植物生长发育和环境适应等方面起着极为重要的作用．本研究对拟南芥ago1-27突变体进行了RNA-seq，鉴定到几千个差异表达基因，并且miRNA靶基因倾向于在ago1-27中表达上调．ago1-27 RNA-seq结合降解组分析鉴定到新的miRNA靶基因，如miR396靶向半胱氨酸蛋白酶基因和miR167靶向编码AT-hook DNA结合蛋白的基因等．
Abstract:
miRNAs play important roles in plant development and adaptation to environment. We conduct the RNA-seq of Arabidopsis ago1-27 mutant and has identified thousands of differentially expressed genes. The study shows that miRNA targets tend to be upregulated in the ago1-27 mutant. New miRNA targets are identified by ago1-27 RNA-seq combined with degradome analysis. For examples, miR396 targets cysteine proteinase genes and miR167 targets a gene encoding AT-hook DNA-binding protein.

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备注/Memo

备注/Memo:
Received：2016-12-23；Accepted：2016-12-27
Foundation：National Natural Science Foundation of China (31571332, 31210103901, 91440105)
Corresponding author：Academician of American Academy of Sciences, Professor Chen Xuemei. E-mail: xuemei.chen@ucr.edu
Citation：Ma Xuan, Li Shengben, Mo Beixin, et al. RNA-seq analysis on Arabidopsis ago1-27 mutant［J］. Journal of Shenzhen University Science and Engineering, 2017, 34(1): 27-32.（in Chinese）
基金项目：国家自然科学基金资助项目（31571332，31210103901，91440105）
作者简介：马轩（1978—），男，深圳大学博士后研究人员. 研究方向：植物miRNA介导的翻译抑制机理. E-mail: xuanma@genetics.ac.cn
引文：马轩，李盛本，莫蓓莘，等. 拟南芥ago1-27突变体的RNA-seq分析［J］. 深圳大学学报理工版，2017，34(1)：27-32.
更新日期/Last Update: 2016-12-30