[1]丁利平,刘冬舟,黎丽,等.脂多糖协同MSU诱导THP1分泌IL-1β和IL-18[J].深圳大学学报理工版,2016,33(6):566-570.[doi:10.3724/SP.J.1249.2016.06566]
 Ding Liping,Liu Dongzhou,Li Li,et al.Synergistic action of LPS on production of IL-1β and IL-18 induced by MSU in THP1 cells[J].Journal of Shenzhen University Science and Engineering,2016,33(6):566-570.[doi:10.3724/SP.J.1249.2016.06566]
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脂多糖协同MSU诱导THP1分泌IL-1β和IL-18()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第33卷
期数:
2016年第6期
页码:
566-570
栏目:
生物工程
出版日期:
2016-11-20

文章信息/Info

Title:
Synergistic action of LPS on production of IL-1β and IL-18 induced by MSU in THP1 cells
文章编号:
201606003
作者:
丁利平12刘冬舟2黎丽1黄钟1
1)深圳大学医学部,广东深圳 518060
2)深圳市人民医院暨南大学第二临床医学院风湿免疫科,广东深圳518020
Author(s):
Ding Liping12 Liu Dongzhou2 Li Li1 and Huang Zhong1
1) School of Medicine, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China
2) Department of Rheumatology & Immunology, Shenzhen People’s Hospital, 2nd Clinical Medical College of Jinan University, Shenzhen 518020, Guangdong Province, P.R.China
关键词:
细胞免疫学尿酸钠结晶脂多糖痛风炎症体白细胞介素-1β白细胞介素-18
Keywords:
cellular immunology monopodium urate crystals lipopolysaccharide gout inflammasome interleukin-1β (IL-1β) interleukin-18 (IL-18)
分类号:
R 392
DOI:
10.3724/SP.J.1249.2016.06566
文献标志码:
A
摘要:
通过合成尿酸钠结晶(monosodium urate crystals, MSU)分析其对人外周血单核细胞系THP1的促炎作用,分别以 0、1.0、10.0和100.0 μmol/L的MSU刺激THP1细胞4 h,检测不同浓度的MSU对炎症体组分Nod 样受体蛋白3(Nod-like receptor protein 3, NLRP3)、半胱氨酸天冬氨酸特异性蛋白水解酶(caspase-1)及促炎因子白细胞介素-1β(interleukin-1β, IL-1β)和IL-18的信使核糖核酸(messenger ribonucleic acid, mRNA)表达的影响. 为研究toll 样受体4(toll like receptor 4,TLR4)的配体脂多糖的作用,用1.0 ng/mL的脂多糖(lipopolysaccharide, LPS)预刺激THP1细胞3 h,再加100.0 μmol/mL的MSU刺激细胞,通过实时荧光定量聚合酶链式反应及酶联免疫吸附试验,检测LPS对MSU诱导的促炎因子IL-1β和IL-18的mRNA及蛋白水平.研究发现,在THP1 细胞中,100.0 μmol/L的MSU即可显著激活炎症体的mRNA,但其对促炎因子IL-1β及IL-18的mRNA表达的诱导作用却不明显,加入1.0 ng/mL LPS预刺激3 h后,MSU诱导的THP1细胞IL-1β和IL-18的表达量显著增加.证明脂多糖对MSU诱导THP1细胞产生IL-1β 和IL-18有协同作用.
Abstract:
The synthesized monosodium urate crystals (MSU) was used for pro-inflammatory analyses in THP1 cells. THP1 cells were treated with 0, 1.0, 10.0 and 100.0 μmol/L MSU for 4 h in order to analyze the effects of MSU on the expression of messenger ribonucleic acid (mRNA) of inflammasome Nod-like receptor protein 3 (NLRP3), caspase-1 and pro-inflammatory cytokines interleukin-1β (IL-1β) and IL-18. To investigate the role of lipopolysaccharide (LPS)-toll like receptor 4 (TLR4) ligand, THP1 cells were pre-treated with 1.0 ng/mL LPS 3 h, and then stimulated with 100.0 μmol/mL MSU. The mRNA and protein expression of pro-inflammatory cytokines IL-1β and IL-18 induced by LPS and MSU were detected using quantitative real-time polymerase chain reaction (qPCR) and enzyme linked immunosorbent assay (ELISA), respectively. The results show that 100.0 μmol/L MSU could activate the mRNA expression of inflammasome, but has comparatively less effect on the mRNA expression of IL-1β and IL-18 in THP1 cells. The productions of IL-1β and IL-18 were significantly amplified in THP1 cells pretreated with 1.0 ng/mL LPS for 3 h before the MSU stimulation. Results suggest that MSU can act in synergy with LPS to enhance the release of IL-1β and IL-18 in mononuclear cells.

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相似文献/References:

[1]丁利平,黎丽,刘冬舟,等.IL-37对白塞病患者单核细胞分泌炎症因子的影响[J].深圳大学学报理工版,2016,33(5):447.[doi:10.3724/SP.J.1249.2016.05447]
 Ding Liping,Li Li,Liu Dongzhou,et al.The anti-inflammatory effects of interleukin-37 on active Behcet’s disease[J].Journal of Shenzhen University Science and Engineering,2016,33(6):447.[doi:10.3724/SP.J.1249.2016.05447]

备注/Memo

备注/Memo:
Received:2016-07-07;Accepted:2016-09-12
Foundation:Postdoctoral Science Foundation of China (2015M572370); Shenzhen Science and Technology Research Foundation(JCYJ20130402092657775)
Corresponding author:Chief physician Liu Dongzhou. E-mail: liu_dz2001@sina.com; Postdoctornal Li Li. E-mail: wlili19860318123@163.com
Citation:Ding Liping, Liu Dongzhou, Li Li, et al. Synergistic action of LPS on production of IL-1β and IL-18 induced by MSU in THP1 cells[J]. Journal of Shenzhen University Science and Engineering, 2016, 33(6): 566-570.(in Chinese)
基金项目:中国博士后科学基金资助项目(2015M572370); 深圳市科技计划资助项目(JCYJ20130402092657775)
作者简介:丁利平(1984—),女,深圳大学博士后研究人员.研究方向:自身免疫及炎症. E-mail: 13552432187@163.com
引文:丁利平,刘冬舟,黎丽,等. 脂多糖协同MSU诱导THP1分泌IL-1β和IL-18[J]. 深圳大学学报理工版,2016,33(6):566-570.
更新日期/Last Update: 2016-10-31