[1]丁利平,黎丽,刘冬舟,等.IL-37对白塞病患者单核细胞分泌炎症因子的影响[J].深圳大学学报理工版,2016,33(5):447-451.[doi:10.3724/SP.J.1249.2016.05447]
 Ding Liping,Li Li,Liu Dongzhou,et al.The anti-inflammatory effects of interleukin-37 on active Behcet’s disease[J].Journal of Shenzhen University Science and Engineering,2016,33(5):447-451.[doi:10.3724/SP.J.1249.2016.05447]
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IL-37对白塞病患者单核细胞分泌炎症因子的影响()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第33卷
期数:
2016年第5期
页码:
447-451
栏目:
生物工程
出版日期:
2016-09-24

文章信息/Info

Title:
The anti-inflammatory effects of interleukin-37 on active Behcet’s disease
文章编号:
201605002
作者:
丁利平黎丽刘冬舟黄钟
1) 深圳大学医学部,广东深圳 518060
2) 暨南大学第二临床医学院-深圳市人民医院风湿免疫科,广东深圳518020
Author(s):
Ding Liping Li Li Liu Dongzhou and Huang Zhong
1) College of Medicine, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China
2) Department of Rheumatology & Immunology, Shenzhen people’s Hospital, the Second Clinical Medical College of Jinan University, Shenzhen 518020, Guangdong Province, P.R.China
关键词:
细胞免疫学白塞病白介素37interleukin-1β interleukin-17A tumor necrosis factor alpha
Keywords:
cellular immunology Behcet’s disease interleukin-37 interleukin-1β interleukin-17A tumor necrosis factor alphacellular immunology Behcet’s disease interleukin-37 interleukin-1β interleukin-17A tumor necrosis factor alpha
分类号:
R 392
DOI:
10.3724/SP.J.1249.2016.05447
文献标志码:
A
摘要:
通过合成白介素37(interleukin-37, IL-37)重组蛋白,对分离的活动期白塞病患者的外周血单核细胞(peripheral blood mononuclear cells, PBMC)进行预刺激,研究IL-37对白塞病炎症的影响.通过荧光定量PCR及酶联免疫吸附试验对白塞病人及正常人的外周血单核细胞中炎症细胞因子interleukin-1β(IL-1β)、interleukin-17A(IL-17A)和tumor necrosis factor alpha(TNF-α)的基因和蛋白表达水平进行分析,发现IL-37能有效降低白塞病PBMC中IL-1β、IL-17A及TNF-α的mRNA表达,减少炎性细胞因子IL-1β、IL-17A及TNF-α的细胞外分泌,从而证明IL-37在体外对白塞病的炎症具有抑制作用.
Abstract:
The recombinant protein interleukin-37 (IL-37) was synthesized for anti-inflammatory analyses in peripheral blood mononuclear cells (PBMC) from Behcet’s disease (BD). The protein levels of inflammatory cytokines such as interleukin-1β (IL-1β), interleukin-17A (IL-17A) and tumor necrosis factor alpha (TNF-α) in cell culture supernatant of patients with BD and healthy controls (HC) were measured by means of enzyme-linked immunosorbent assays (ELISA). The relative mRNA expression of these cytokines in PBMC was detected through real time quantitative PCR. We found that the protein and mRNA levels of inflammatory cytokines in active BD patients are significantly suppressed by recombinant IL-37 protein in vitro but not in the HCs. This finding suggests that IL-37 can significantly inhibit the inflammation in active BD patients.

参考文献/References:

[1] CantariniL, Lopalco G, Caso F, et al. Effectiveness and tuberculosis-related safety profile of interleukin-1 blocking agents in the management of Behcet’s disease[J]. Autoimmunity Reviews, 2015, 14(1): 1-9.
[2] Mendoza-Pinto C, Garcia-Carrasco M, Jimenez-Hernandez M, et al. Etiopathogenesis of Behcet’s disease[J]. Autoimmunity Reviews, 2010, 9(4): 241-245.
[3] Kapsimali V D, Kanakis M A, Vaiopoulos G A, et al. Etiopathogenesis of Behcet’s disease with emphasis on the role of immunological aberrations[J]. Clinical Rheumatology, 2010, 29(11): 1211-1216.
[4] Pineton de C M, Wechsler B, Geri G, et al. New insights into the pathogenesis of Behcet’s disease[J]. Autoimmunity Reviews, 2012, 11(10): 687-698.
[5] Emiroglu N, Cengiz F P, Erdem G B. Serum IL-17A in Behcet’s disease[J]. Postepy Dermatol Alergol, 2015, 32(5): 358-361.
[6] Zhou Zhenyuan, Chen S L, Shen Nan, et al. Cytokines and Behcet’s disease[J]. Autoimmunity Reviews, 2012, 11(10): 699-704.
[7] Xu Wangdong, Zhao Yi, Liu Yi. Insights into IL-37, the role in autoimmune diseases[J]. Autoimmunity Reviews, 2015, 14: 1170-1175.
[8] Hatemi G, Yazici Y, Yazici H. Behet’s syndrome[J]. Rheumatic Diseases Clinics of North America, 2013, 39(2): 245-261.
[9] Leng Ruixue, Chen Guimei, Pan Haifeng, et al. The role of IL-23/IL-17 axis in the etiopathogenesis of Behcet’s disease[J]. Clinical Rheumatology, 2010, 29(10): 1209.
[10] Touzot M, Cacoub P, Bodaghi B, et al. IFN-α induces IL-10 production and tilt the balance between Th1 and Th17 in Behet disease[J]. Autoimmunity Reviews, 2015, 14(5): 370-375.
[11] Vitale A, Rigante D, Lopalco G, et al. Interleukin-1 Inhibition in Behcet’s disease[J]. Israel Medical Association Journal, 2016, 18(3/4): 171-176.
[12] Chan E, Sangle S R, Coghlan J G, et al. Pulmonary artery aneurysms in Behcet’s disease treated with anti-TNFα: aa case series and review of the literature[J]. Autoimmunity Reviews, 2016, 15(4): 375-378.
[13] Ye Liang, Ji Ling, Wen Zhongyang, et al. IL-37 inhibits the production of inflammatory cytokines in peripheral blood mononuclear cells of patients with systemic lupus erythematosus: its correlation with disease activity[J]. Journal of Translational Medicine, 2014, 12(1): 69.
[14] Wang Weiqing, Dong Kui, Zhou Lu, et al. IL-37b gene transfer enhances the therapeutic efficacy of mesenchumal stromal cells in DSS-induced colitis mice[J]. Acta Pharmacologica Sinica, 2015, 36(11): 1377-1387.
[15] Chen Bingni, Huang Kunzhao, Ye Liang, et al. Interleukin-37 is increased in ankylosing spondylitis patients and associated with disease activity[J]. Journal of Translational Medicine, 2015, 13(1): 36.
[16] Bouali E, Kaabachi W, Hamzaoui A, et al. Interleukin-37 expression is decreased in Behcet’s disease and is associated with inflammation[J]. Immunology Letters, 2015, 167(2): 87-94.

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备注/Memo

备注/Memo:
Received:2016-07-07;Accepted:2016-08-15
Foundation:Postdoctoral Science Foundation of China (2015M572370); Shenzhen Science and Techndogy Research Foundation (JCYJ20130402092657775)
Corresponding author:Professor Liu Dongzhou. E-mail: liu_dz2001@sina.com;
Postdoctoral Li Li. E-mail: wlili19860318123@163.com
Citation:Ding Liping, Li Li, Liu Dongzhou, et al. The anti-inflammatory effects of interleukin-37 on active Behcet’s disease[J]. Journal of Shenzhen University Science and Engineering, 2016, 33(5): 447-451.(in Chinese)
基金项目:中国博士后科学基金资助项目(2015M572370);深圳市科技计划资助项目(JCYJ20130402092657775)
作者简介:丁利平(1984—)女,深圳大学博士后研究人员.研究方向:自身免疫及炎症. E-mail: 13552432187@163.com
引文:丁利平,黎丽,刘冬舟,等.IL-37对白塞病患者单核细胞分泌炎症因子的影响[J]. 深圳大学学报理工版,2016,33(5):447-451.
更新日期/Last Update: 2016-09-08