[1]倪卓,黄苇殷,王应,等.PEEK-SPEEK-HA复合材料与U2OS细胞相容性研究[J].深圳大学学报理工版,2016,33(1):1-9.[doi:10.3724/SP.J.1249.2016.01001]
 Ni Zhuo,Huang Weiyin,Wang Ying,et al.Experiments on biocompatibility of PEEK-SEEK-HA composite materials with U2OS cells[J].Journal of Shenzhen University Science and Engineering,2016,33(1):1-9.[doi:10.3724/SP.J.1249.2016.01001]
点击复制

PEEK-SPEEK-HA复合材料与U2OS细胞相容性研究()
分享到:

《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第33卷
期数:
2016年第1期
页码:
1-9
栏目:
化学与化工
出版日期:
2016-01-20

文章信息/Info

Title:
Experiments on biocompatibility of PEEK-SEEK-HA composite materials with U2OS cells
文章编号:
201601001
作者:
倪卓黄苇殷王应吴耿锋潘田光
深圳大学化学与环境工程学院,广东深圳518060
Author(s):
Ni ZhuoHuang WeiyinWang YingWu Gengfengand Pan Tianguang
College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China
关键词:
聚醚醚酮-羟基磷灰石复合材料U2OS骨瘤细胞细胞增殖细胞形态细胞黏附细胞凋亡活性氧
Keywords:
polyetheretherketone-hydroxyapatite (PEEK-HA) composite U2OS cell cell morphology proliferation cell adhesion cell apoptosis reactive oxygen species (ROS)
分类号:
R 318.08
DOI:
10.3724/SP.J.1249.2016.01001
文献标志码:
A
摘要:
体外培养U2OS细胞,通过噻唑蓝(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltet razolium bromide, MTT)法研究新型聚醚醚酮-羟基磷灰石(polyetheretherketone-hydroxyapatite, PEEK-HA)生物复合材料对细胞增殖的影响.研究发现,聚醚醚酮-磺化聚醚醚酮-羟基磷灰石(polyetheretherketone-sulfnated polyetheretherketone-hydroxyapatite, PEEK-SPEEK-HA)复合材料的细胞增殖作用较PEEK-HA复合材料强,且随着HA含量的增加细胞增殖作用增强,材料浸提液质量浓度为4~8 mg/mL时对细胞有较好的增殖促进作用.复合材料毒性级别为Ⅰ,对细胞无毒性.测定黏附率研究该材料对U2OS细胞的黏附特性,PEEK-SPEEK-HA复合材料随着HA含量的增大,细胞黏附率增加,高于PEEK-HA复合材料组,表明加入HA和SPEEK能改善复合材料的黏附性能.采用流式细胞术研究材料对细胞凋亡的影响,发现加入SPEEK的PEEK-HA复合材料能抑制细胞发生早期凋亡,从而降低凋亡率.激光共聚焦和流式细胞仪检测该材料对细胞活性氧的影响发现,SPEEK引入复合材料能够降低细胞活性氧水平,降低细胞毒性. 扫描电镜实验表明,U2OS细胞能够在PEEK-SPEEK-HA复合材料表面黏附、伸展和生长.
Abstract:
Effects of polyetheretherketone-hydroxyapatite (PEEK-HA) composites and polyetheretherketone-sulfnated polyetheretherketone-hydroxyapatite (PEEK-SPEEK-HA) composites on U2OS cells are studied by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltet razolium bromide (MTT) method. It is found that cell proliferation in PEEK-SPEEK-HA composites is broader than that in PEEK-HA composites. With an increase of HA content in PEEK-HA composites, cell proliferation accelerates. The material of sulfnated polyetheretherketone promotes cell proliferation significantly at 4-8 mg/mL concentration. A cytotoxicity test shows that these bio-materials are non-toxic to U2OS cells and the U2OS cells can adapt to these materials. With an increase of HA content, the cell adhesion rate increases and is higher in PEEK-SPEEK-HA composites than in PEEK-HA composites, which indicates that adding HA and SPEEK in PEEK composite could improve the adhesion property. Cell apoptosis test by the flow cytometry (FCM) indicates that the addition of SPEEK in PEEK-HA composites can inhibit the early apoptosis thus decreasing the apoptosis rate. A test on the effect of SPEEK on the reactive oxygen species (ROS) of U2OS cells by using the flow cytometry (FCM) and the confocal laser scanning microscopy (CLSM) shows that SPEEK in PEEK-HA composites can decrease the ROS level, and reduce the cytotoxicity. Scanning electron microscope (SEM) results show that U2OS cells can adhere, stretch and grow on surfaces of PEEK-SPEEK-HA composites.

参考文献/References:

[1] Boden S D.Bone repair and enhancement clinical trial design[J]. Clinical Orthopedics and Related Research, 1998, 355(355S):S336-S346.
[2] 倪卓,王应,王双,等.PEEK/HA 生物复合材料对MG-63细胞的作用[J]. 深圳大学学报理工版,2014,31(3):258-265.
Ni Zhuo,Wang Ying,Wang Shuang,et al. Influence of PEEK/HA composites on MG-63 cells[J]. Journal of Shenzhen University Science and Engineering,2014,31(3):258-265.(in Chinese)
[3] 倪卓,杨莎,王应,等.骨替代材料的研究方法及其进展[J].深圳大学学报理工版,2015, 32(4):331-342.
Ni Zhuo,Yang Sha,Wang Ying,et al. Research methods and advances on bone substitute materials[J]. Journal of Shenzhen University Science and Engineering,2015,32(4):331-342.(in Chinese)
[4] Ong J L, Chan D C. Hydroxyapatite and their use as coatings in dental implants[J]. Critical Reviews in Biomedical Engineering, 2000, 28(5):667-707.
[5] 倪卓,王应,华文语,等.PEEK/HA生物复合材料的热稳定性研究[J]. 深圳大学学报理工版,2012, 29(6):522-526.
Ni Zhuo,Wang Ying,Hua Wenyu, et al. Thermal stability of PEEK/HA biological composite materials[J]. Journal of Shenzhen University Science and Engineering,2012, 29(6):522-526.(in Chinese)
[6] Rigby R B. Victrex polyethersulfone (PES) and victrex polyetheretherketone (PEEK)[R]. Mississauga, Canada: Society of Plastics Engineers, Brookfield Center, CT, USA, 1984.
[7] Godara A,Raabe D, Green S,et al. The influence of sterilization processes on the micromechanical properties of carbon fiber enforced PEEK composites for bone implant applications[J]. Acta Biomaterial,2007, 3(2):209-220.
[8] 李跃文. 聚醚醚酮复合材料的研究进展[J].化工新型材料, 2009, 37(3):37-40.
Li Yuewen. Advance in poly (ether ether ketone)-based composites[J]. New Chemical Materials, 2009, 37(3):37-40.(in Chinese)
[9] Bakar M, Cheong P, Khor K. Mechanical properties of injection molded hudroxyap-tite-polyetheretherketone biomaterials[J]. Composites Science and Technology, 2003, 63(3):421-425.
[10] Goyal R K, Tiwari A N, Negi Y S, et al. Novel high performance Al2O3/poly(ether ether ketone) nanocomposites for electronics applications[J].Composites Science and Technology, 2007,67(9):1802-1812.
[11] Sayes C M, Liang Feng, Hudson J L, et al. Functionalization density dependence of single-walled carbon nanotubes cytotoxicity in vitro[J]. Toxicology Letters, 2006, 161(2): 135-142.
[12] 倪卓,刘士德,王应,等. PEEK/HA生物材料对成纤维细胞3T3的影响[J].深圳大学学报理工版,2013,30(4):392-397.
Ni Zhuo,Liu Shide,Wang Ying, et al. Influences of 3T3 cells for PEEK/HA composite[J]. Journal of Shenzhen University Science and Engineering,2013,30(4):392-397.(in Chinese)
[13] 王建营,朱治国,孙家跃,等. 聚醚醚酮人造骨关节材料研究[J].化学世界,2004,45(1):53-54.
Wang Jangying. Zhu Zhiguo, Sun Jiayue, et al. The study of PEEK artificial bone joint[J]. Chemical World,2004,45(1):53-54.(in Chinese)
[14] Zhu Xiaolong, Chen Jun, Scheideler I, et al. Effects of topography and composition of titanium surface oxides on osteoblast responses[J]. Biomaterials, 2004, 25(18): 4087-4103.
[15] Li Ning, Xia Tian, Nel A E. The role of oxidative stress in ambient particulate matter-induced lung diseases and its implications in the toxicity of engineered nanoparticles[J]. Free Radical Biology & Medicine, 2008, 44(9): 1689-1699.

备注/Memo

备注/Memo:
Received:2015-10-28;Accepted:2015-12-16
Foundation:National Natural Science Foundation of China (51378315); Science and Technology Innovation Project of Guangdong Provincial Educational Department (2013KJCX0163)
Corresponding author:Professor Ni Zhuo.E-mail: royzhuoni@hotmail.com
Citation:Ni Zhuo, Huang Weiyin, Wang Ying, et al.Experiments on biocompatibility of PEEK-SEEK-HA composite materials with U2OS cells[J]. Journal of Shenzhen University Science and Engineering, 2016, 33(1): 1-9.(in Chinese)
基金项目:国家自然科学基金资助项目(51378315);广东省教育厅科技创新资助项目(2013KJCX0163)
作者简介:倪卓(1965—),男,深圳大学教授、博士生导师. 研究方向:生物材料和功能材料. E-mail: royzhuoni@hotmail.com
引文:倪卓,黄苇殷,王应,等. PEEK-SPEEK-HA复合材料与U2OS细胞相容性研究[J]. 深圳大学学报理工版,2016,33(1):1-9.
更新日期/Last Update: 2016-01-14