[1]高阳,刘国宝,刘科,等.大豆ASR表达提高酵母和烟草细胞对Cu2+耐受力[J].深圳大学学报理工版,2015,32(2):137-144.[doi:10.3724/SP.J.1249.2015.02137]
 Gao Yang,Liu Guobao,Liu Ke,et al.Expression of ASR gene confers copper tolerance to yeast mutant and transgenic tobacco BY-2 cells[J].Journal of Shenzhen University Science and Engineering,2015,32(2):137-144.[doi:10.3724/SP.J.1249.2015.02137]
点击复制

大豆ASR表达提高酵母和烟草细胞对Cu2+耐受力()
分享到:

《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第32卷
期数:
2015年第2期
页码:
137-144
栏目:
生物工程
出版日期:
2015-03-20

文章信息/Info

Title:
Expression of ASR gene confers copper tolerance to yeast mutant and transgenic tobacco BY-2 cells
文章编号:
201502004
作者:
高阳刘国宝刘科李冉辉郑易之
深圳市微生物基因工程重点实验室,深圳大学生命科学学院,深圳 518060
Author(s):
Gao Yang Liu Guobao Liu Ke Li Ranhui and Zheng Yizhi
Shenzhen Key Laboratory of Microbiology and Gene Engineering, College of Life Science, Shenzhen University, Shenzhen 518060, P.R.China
关键词:
植物基因工程GmASR基因转基因烟草重组突变体酵母抗金属离子胁迫金属离子结合蛋白
Keywords:
plant genetic engineering GmASR gene transgenic tobacco BY-2 cells recombinant mutant yeast metal ion stress tolerance metal-binding protein
分类号:
Q 943.2;Q 786
DOI:
10.3724/SP.J.1249.2015.02137
文献标志码:
A
摘要:
利用实时荧光定量聚合酶链式反应(polymerase chain reaction,PCR)技术,证明大豆幼苗在150 μmol/L CuSO4胁迫3 h后,其叶和根内GmASR基因表达均上调.将大豆GmASR基因转化Cu2+敏感型酵母菌ΔCUP2和烟草悬浮细胞BY-2,GmASR蛋白的表达可增强重组酵母和转基因烟草细胞抗Cu2+胁迫的能力.利用大肠杆菌表达体系表达、分离并纯化大豆GmASR蛋白,体外实验证明,GmASR蛋白可与Cu2+结合,并具有清除羟基自由基的能力.研究结果推测,ASR蛋白可通过螯合Cu2+降低细胞内Cu2+浓度,提高植物对Cu2+胁迫的耐受力.
Abstract:
Expression analysis by quantitative real-time polymerase chain reaction (PCR) shows that GmASR is identified with increased expression in the roots and leaves of soybean seedlings under 150 μmol/L CuSO4 stress for 3 h. The GmASR gene is introduced into Cu2+ sensitive yeast ΔCUP2 and tobacco suspension BY-2 cells, and we find that expression of GmASR protein could improve copper tolerance of the recombinant yeast mutant and transgenic tobacco BY-2 cells. GmASR protein is induced, separated and purified by using the recombinant E.Coli. It is confirmed that the GmASR protein can bind Cu2+ and also inhibit hydroxyl radical generation in vitro. We can speculate that the GmASR protein would enhance the plant tolerance of copper stress by chelating Cu2+, reducing intracellular Cu2+ concentration and oxidant damage.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(31370289);深圳市科技创新委资助项目(JCYJ20120614085333654)
作者简介:高阳(1989—),女(汉族),湖北省孝感市人,深圳大学硕士研究生.E-mail:gaoy822@163.com
引文:高阳,刘国宝,刘科,等. 大豆ASR表达提高酵母和烟草细胞对Cu2+耐受力[J]. 深圳大学学报理工版,2015,32(2):137-144.
Received:2014-12-26;Accepted:2015-01-30
Foundation:National Natural Science Foundation of China (31370289);Science and Technology Project of Shenzhen(JCYJ20120614085333654)
Corresponding author:Professor Zheng Yizhi.E-mail:yzzheng@szu.edu.cn
Citation:Gao Yang, Liu Guobao, Liu Ke, et al. Expression of ASR gene confers copper tolerance to yeast mutant and transgenic tobacco BY-2 cells[J]. Journal of Shenzhen University Science and Engineering, 2015, 32(2): 137-144.(in Chinese)
更新日期/Last Update: 2015-03-12