[1]李冰石,姜婷婷.聚集诱导发光在生物/化学传感器的应用[J].深圳大学学报理工版,2014,31(1):88-95.[doi:10.3724/SP.J.1249.2014.01088]
　Li Bingshi and Jiang Tingting.The application of aggregation induced emission as bio/chemosensors[J].Journal of Shenzhen University Science and Engineering,2014,31(1):88-95.[doi:10.3724/SP.J.1249.2014.01088]
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聚集诱导发光在生物/化学传感器的应用()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第31卷
期数:
2014年第1期
页码:
88-95
栏目:
材料科学
出版日期:
2014-01-14

文章信息/Info

Title:
The application of aggregation induced emission as bio/chemosensors
文章编号:
20140113
作者:
李冰石; 姜婷婷
深圳大学化学与化工学院，深圳 518060
Author(s):
Li Bingshi and Jiang Tingting
College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen 518060, P.R.China
关键词:
分析化学; 聚集诱导发光; 分子内旋转受限;  生物/化学传感器; 离子及有害物检测; 蛋白质; 荧光; 光开关
Keywords:
analytical chemistry;  aggregation-induced emission;  restricted intramolecular rotation;  bio/chem-sensor;  ion and harmful substance detection;  protein;  fluorescence;  light switch
分类号:
O 661
DOI:
10.3724/SP.J.1249.2014.01088
文献标志码:
A
摘要:
综述聚集诱导发光（aggregation-induced emission，AIE）现象的发现、机理及其在生物/化学探针应用研究方面的进展．指出传统荧光分子溶液浓度过高或分子发生聚集后，往往发生分子荧光淬灭，而AIE现象刚好与之相反，分子发生聚集后荧光会增强数倍或数百倍．AIE现象的本质是分子聚集使分子间相互作用增强，限制了分子内基团的自由旋转，抑制了其非辐射能量的转移，使分子荧光增强．生物分子或待测化学物质可以通过疏溶剂作用、静电作用或配位作用诱导AIE分子发生聚集，使分子荧光显著增强，根据这一“光开关效应” 可以构筑用于蛋白、DNA、金属离子以及有害物质检测的生物/化学传感器．该类新型传感器操作简单便捷，与传统的检测方法相比，在检测灵敏性和选择性方面具有极大的优势．
Abstract:
The finding of aggregation-induced emission (AIE) and development of AIE-based application in bio/chemosensors is reviewed. The conventional chromophoric molecules are usually quenched because of molecular aggregation. However, the AIE molecules are non-emissive in solution, but their luminescence is significantly enhanced when they aggregate or exist in solid state. The essence of AIE is the restriction of intramolecular rotation and enhancement of intermolecular interaction, which blocks the non-emissive energy transfer and therefore leads to an increase of luminescence. Biomolecules or chemical analyte can induce AIE molecules to aggregate with a significant increase of luminescence. This “light-switch” mechanism can be employed to develop bio/chemosensors for protein, DNA and chemical analyte detection. The AIE based bio/chemosensors are easy to operate. They also have the advantages of high sensitivity and selectivity compared with conventional detection methods.

参考文献/References:

［1］ Forster T, Kasper K. Ein konzentrationsumschlag der fluoreszenz［J］. Zeitschrift für Physikalische Chemie, 1954, 1(5/6): 275-277.
［2］ Birks J B. Photophysics of Aromatic Molecules［M］. London: Wiley, 1970.
［3］ Slav1′k J. Fluorescence Microscopy and Fluorescent Probes［M］. New York: Plenum, 1996.
［4］ Valeur B. Molecular Fluorescence: Principle and Applications［M］. Weinheim(German): Wiley-VCH, 2002.
［5］ Sapsford K E, Berti L, Medintz I L. Materials for fluorescence resonance energy transfer analysis：beyond traditional donor-acceptor combinations［J］. Angewandte Chemie International Edition, 2006, 45(28): 4562-4589.
［6］ Borisov S M, Wolfbeis O S. Optical biosensors［J］. Chemical Society Reviews, 2008, 108(2): 423-461.
［7］ Domaille D W, Que E L, Chang C J. Synthetic fluorescent sensors for studying the cell biology of metals［J］ . Nature Chemical Biology, 2008, 4(3): 168-175.
［8］ Lim M H, Lippard S J. Metal-based turn-on fluorescent probes for sensing nitric oxide［J］. Accounts of Chemical Research, 2007, 40(1): 41-51.
［9］ Giepmans B N G, Adams S R, Ellisman M H, et al. The fluorescent toolbox for assessing protein location and function［J］. Science, 2006, 312(5771): 217-224.
［10］ Luo J, Xie Z, Lam J W Y, et al. Aggregation-induced emission of 1-methyl-1, 2, 3, 4, 5-pentaphenylsilole［J］. Chemical Communication, 2001(18): 1740-1741.
［11］ Yan Jiming, Qin Anjun, Sun Jingzhi, et al. Application of AIE-active molecules in biosensing［J］. Chinese Sci Bull, 2010, 55(13): 1206-1213.(in Chinese)
闫继明，秦安军，孙景志，等. 聚集诱导发光分子在生物传感检测领域的应用［J］. 科学通报, 2010, 55(13): 1206-1213.
［12］ Tong H, Dong Y. Color-tunable, aggregation-induced emission of a butterfly-shaped molecule comprising a pyran skeleton and two cholesteryl wings［J］. Journal of Physical Chemistry B, 2007, 111(8) : 2000-2007.
［13］ Tong H, Dong Y, Hong Y N, et al. Aggregation-induced emission: effects of molecular structure, solid-state conformation, and morphological packing arrangement on light-emitting behaviors of diphenyldi-benzofulvene derivatives［J］. Journal of Physical Chemistry C, 2007, 111(5): 2287-2294.
［14］ Hong Y N, Lam J W Y, Tang B Z. Aggregation-induced emission: phenomenon, mechanism and applications［J］. Chemical Communication, 2009(29): 4332-4353.
［15］ Hong Y N, Lam J W Y, Tang B Z. Aggregation-induced emission［J］. Chemical Society Reviews, 2011, 40(11): 5361-5388.
［16］ Zhang Shuang, Qin Anjun, Sun Jingzhi, et al. Mechanism study of agreegation-induced emission［J］. Progress in Chemistry, 2011, 23(4): 623-636.（in Chinese）
张双，秦安军，孙景志，等. 聚集诱导发光机理研究［J］. 化学进展, 2011, 23(4): 623-636.
［17］ Yu G, Yin S W, Liu Y Q, et al. Structures, electronic states, photoluminescence, and carrier transport properties of 1, 1-disubstituted 2, 3, 4, 5-tetraphenylsiloles［J］. Journal of the American Chemical Society, 2005, 127(17): 6335-6346.
［18］ Chen J W, Law C C W, Lam J W Y, et al. Synthesis, light emission, nanoaggregation，and restricted intramolecular rotation of 1, 1-substituted 2, 3, 4, 5-tetraphenylsiloles［J］. Chemical Materials, 2003, 15(7): 1535-1546.
［19］ Fan X, Sun J L, Wang F Z, et al. Photoluminescence and electroluminescence of hexaphenylsilole are enhanced by pressurization in the solid state［J］. Chemical Communication, 2008(26): 2989-2991.
［20］ Ren Y, Lam J W Y, Dong Y Q, et al. Enhanced emission efficiency and excited state lifetime due to restricted intramolecular motion in silole aggregates［J］. Journal of Physical Chemistry B, 2005, 109(3): 1135-1140.
［21］ Ren Y, Lam J W Y, Dong Y Q, et al. Studies on the aggregation-induced emission of silole film and crystal by time-resolved fluorescence technique［J］. Chemical Physics Letters, 2005, 402(4): 468-473.
［22］ Li Z, Dong Y Q, Mi B X, et al. Structural control of the photoluminescence of silole regioisomers and their utility as sensitive regiodiscriminating chemosensors and efficient electroluminescent materials［J］. Journal of Physical Chemistry B, 2005, 109(20): 10061-10066.
［23］ Wang M, Zhang G X, Zhang D Q, et al. Fluorescent bio/chemosensors based on silole and tetraphenylethene luminogens with aggregation-induced emission feature［J］. Journal of Materials Chemistry, 2010, 20(10): 1858-1867.
［24］ Zhang Yunsheng, Qing Jiuhong, Xu Caihong. Research progress of application of silole derivatives with aggregation-induced emission［J］. Silicone Material, 2011, 25(3): 190-198.(in Chinese)
张运生, 秦九红, 徐彩虹. 聚集诱导发光的硅杂环戊二烯衍生物的应用研究进展［J］. 有机硅材料, 2011, 25(3): 190-198.
［25］ Zhao Yuezhi, Cai Minmin, Qian Yan, et al. The systems with aggregation induced emission: compounds, emission, mechanisms and their applications［J］. Progress in Chemistry, 化学进展，2013, 25(203): 296-321.(in Chinese)
赵跃智, 蔡敏敏, 钱妍, 等. 聚集诱导发光体系：化合物种类、发光机制及其应用［J］. 2013, 25(203): 296-321.
［26］ Ma Qingyu, Guan Ruifang, Li Guozhong, et al. Progress in the synthesis and applications of silole compounds［J］. Chinese Journal of Organic Chemistry, 2011, 31(9): 1395-1405.(in Chinese)
马庆宇, 关瑞芳, 李国忠, 等. 硅杂环戊二烯的合成及应用进展［J］. 有机化学，2011, 31(9): 1395-1405.
［27］ Xia Jing, Wu Yanmei, Zhang Yaling, et al. Recent progress of tetraphenylethenes with aggregation-induced emission［J］. Imaging Science and Photochemisty, 2012, 30(1): 9-25.(in Chinese)
夏晶，吴燕梅，张亚玲, 等. 具有聚集诱导发光特性的四苯基乙烯研究进展［J］. 影像科学与光化学, 2012, 30(1): 9-25.
［28］ Zhao Guosheng, Shi Chuanxing, Guo Zhiqian, et al. Recent application progress on aggregation-induced emission［J］. Chinese Journal of Organic Chemistry, 2012, 32(9): 1620-1632.(in Chinese)
赵国生，史川兴，郭志前，等. 聚集诱导发光应用研究进展［J］. 有机化学，2012, 32(9): 1620-1632.
［29］ Wang M, Zhang D, Zhang G, et al. Fluorescence turn-on detection of DNA and label-free fluorescence nuclease assay based on the aggregation-induced emission of silole［J］. Analytical Chemistry, 2008, 80(16): 6443-6448.
［30］ Wang M, Zhang D, Zhang G, et al. The convenient fluorescence turn-on detection of heparin with a silole derivative featuring an ammonium group［J］. Chemical Communication, 2008(37): 4469-4471.
［31］ Zhao M, Wang M, Liu H, et al. Continuous on-site label-free ATP fluorometric assay based on aggregation-induced emission of silole［J］. Langmuir, 2009, 25(2): 676-678.
［32］ Tong H, Hong Y N, Don Y Q, et al. Fluorescent “light-up” bioprobes based on tetraphenylethylene derivatives with aggregation-induced emission characteristics［J］. Chemical Communication, 2006(35) : 3705-3707.
［33］ Tong H, Hong Y, Dong Y, et al. Protein detection and quantitation by tetraphenylethene-based fluorescent probes with aggregation-induced emission characteristics［J］. Journal of Physical Chemistry B, 2007, 111(40): 11817-11823.
［34］ Wang M，Gu X，Zhang G，et al.Convenient and continuous fluorometric assay method for acetylcholinesterase and inhibitor screening based on the aggregation-induced emission［J］. Analytical Chemistry , 2009, 81(11): 4444-4449.
［35］ Whitehouse P J, Price D L, Struble R G, et al. Alzheimer’s disease and senile dementia: loss of neurons in the basal forebrain［J］. Science, 1982, 215(4537): 1237-1239.
［36］ Peng L, Zhang G, Zhang D Q, et al. A fluorescence “turn-on” ensemble for acetylcholinesterase activity assay and inhibitor screening［J］. Organic Letters, 2009, 11(17): 4014-4017.
［37］ Hong Y N, Feng C, Yu Y, et al. Quantitation, visualization, and monitoring of conformational transitions of human serum albumin by a tetraphenylethene derivative with aggregation-induced emission characteristics［J］. Analytical Chemistry, 2010, 82(16): 7035-7043.
［38］ Xue W, Zhang G X, Zhang D Q, et al. A new fluorometric turn-on assay for trypsin and inhibitor screening with tetraphenylethene compounds［J］. Organic Letters, 2010, 12(10): 2274-2277.
［39］ Hong Y N，Haussler M，Lam J W Y，et al.Label-free fluorescent probing of G-quadruplex formation and real-time monitoring of DNA folding by a quaternized tetraphenylethene salt with aggregation-induced emission characteristics［J］. Chemistry-A Europen Journal, 2008, 14(21): 6428-6437.
［40］ Sun F, Zhang G X, Zhang D X, et al. Aqueous fluorescence turn-on sensor　for Zn2+ with a tetraphenylethylene compound［J］. Organic Letters, 2011, 13(24): 6378-6381.
［41］ Hong Y N, Chen S J, Liu J Z, et al. Fluorogenic Zn(II) and chromogenic Fe(II) sensors based on terpyridine-substituted tetraphenylethenes with aggregation-induced emission characteristics［J］. Applied Materials & Interfaces, 2011, 3(9): 3411-3418.
［42］ Liu L, Zhang G X, Xiang J F, et al. Fluorescence “turn on” chemosensors for Ag+ and Hg2+ based on tetraphenylethylene motif featuring adenine and thymine moieties［J］. Organic Letters, 2008, 10(20): 4581-4584.
［43］ Peng L H, Wang M, Zhang G X, et al. A fluorescence turn-on detection of cyanide in aqueous solution based on the aggregation-induced emission［J］. Organic Letters, 2009, 11(9): 1943-1946.
［44］ Toal S J, Jones K A, Magde D, et al. Luminescent silole nanoparticles as chemoselective sensors for Cr(VI)［J］. Journal of the American Chemical Society, 2005, 127(33): 11661-11665.
［45］ Yuan W Z, Hu R R, Lam J W Y, et al. Conjugated hyperbranched poly(aryleneethynylene)s: synthesis, photophysical properties, superquenching by explosive, photopatternability, and tunable high refractive indices［J］. Chemistry-A Europen Journal, 2012, 18(10): 2847-2856.
［46］ Hu R R, Lam J W Y, Liu J Z, et al. Hyperbranched conjugated poly(tetraphenylethene)：synthesis，aggregation-induced emission, fluorescent photopatterning, optical limiting and explosive detection［J］. Polymer Chemistry, 2012, 3(6): 1481-1489.
［47］ Li H K, Wang J, Sun J Z, et al. Metal-free click polymerization of propiolates and azides: facile synthesis of functional poly(aroxycarbonyltriazole)s［J］. Polymer Chemistry, 2012, 3(4): 1075-1083.
［48］ Qin A J, Tang L, Lam J W Y, et al. Metal-free click polymerization: synthesis and photonic properties of poly(aroyltriazole)s［J］. Advanced Functional Materials, 2009, 19(12): 1891-1900.
［49］ Li D D, Liu J Z, Liang Z Q, et al. Supersensitive detection of explosives by recyclable AIE luminogen-functionalized mesoporous materials［J］. Chemical Communication, 2012, 48(57): 7167-7169.
［50］ Xu B W, Wu X F, Li H B, et al. Selective detection of TNT and picric acid by conjugated polymer film sensors with donor acceptor architecture［J］. Macromolecules, 2011, 44(13): 5089-5092.
［51］ Liu J Z, Zhong Y C, Lam J W Y, et al. Hyperbranched conjugated polysiloles: synthesis，structure，aggregation-enhanced emission, multicolor fluorescent photopatterning, and superamplified detection of explosives［J］. Macromolecules, 2010, 43(11): 4921-4936.
［52］ Qin A J, Lam J W Y, Tang L, et al. Polytriazoles with aggregation-induced emission characteristics: synthesis by click polymerization and application as explosive chemosensors［J］. Macromolecules, 2009, 42(5): 1421-1424.
［53］ Yuan W Z, Zhao H, Shen Y, et al. Luminogenic polyacetylenes and conjugate polyelectrolytes: synthesis, hybridization with carbon nanotubes, aggregation-induced emission, superamplification in emission quenching by explosives, and fluorescent assay for protein quantitation［J］. Macromolecules, 2009, 42(24): 9400-9411.
［54］ Li H K, Mei J, Wang J, et al. Facile synthesis of poly(aroxycarbonyltriazole)s with aggregation-induced emission characteristics by metal-free click polymerization［J］. Science China Chemistry, 2011, 54(4): 611-616.

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备注/Memo

备注/Memo:
Received：2013-05-09；Revised：2013-11-25；Accepted：2013-12-15
Foundation：National Natural Science Foundation of China(21104046); Outstanding Youth Foundation of Shenzhen(JC201005250038A)
Corresponding author：Professor Li Bingshi. E-mail: phbingsl@yahoo.com
Citation：Li Bingshi，Jiang Tingting. The application of aggregation induced emission as bio/chemosensors［J］. Journal of Shenzhen University Science and Engineering, 2014, 31(1): 88-95.（in Chinese）
基金项目：国家自然科学基金资助项目 (21104046)；深圳市杰出青年基金资助项目(JC201005250038A)
作者简介：李冰石（1972-）， 女（汉族），黑龙江省哈尔滨市人，深圳大学教授. E-mail:phbingsl@yahoo.com
引文：李冰石，姜婷婷. 聚集诱导发光在生物/化学传感器的应用［J］. 深圳大学学报理工版，2014，31（1）：88-95.
更新日期/Last Update: 2014-01-08