[1]倪卓,刘士德,王应,等.PEEK-HA生物材料对成纤维细胞3T3的影响[J].深圳大学学报理工版,2013,30(No.4(331-440)):392-397.[doi:10.3724/SP.J.1249.2013.04392]
 Ni Zhuo,Liu Shide,Wang Ying,et al.Influence of 3T3 cells for PEEK-HA composite[J].Journal of Shenzhen University Science and Engineering,2013,30(No.4(331-440)):392-397.[doi:10.3724/SP.J.1249.2013.04392]
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PEEK-HA生物材料对成纤维细胞3T3的影响()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第30卷
期数:
2013年No.4(331-440)
页码:
392-397
栏目:
材料科学
出版日期:
2013-07-12

文章信息/Info

Title:
Influence of 3T3 cells for PEEK-HA composite
文章编号:
20130408
作者:
倪卓1刘士德2王应2刘学3姜振华4
1) 深圳大学化学与化工学院,深圳 518060
2) 深圳大学生命科学学院,深圳 518060
3) 深圳市第六人民医院,深圳 518067
4)吉林大学化学系,长春 130012
Author(s):
Ni Zhuo1 Liu Shide2 Wang Ying2 Liu Xue3 and Jiang Zhenhua4
1) College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen 518060, P.R.China
2) College of Life Science, Shenzhen University, Shenzhen 518060, P.R.China
3) Shenzhen Sixth People’s Hospital, Shenzhen 518067, P.R.China
4) Department of Chemistry, Jilin University, Changchun 130012, P.R.China
关键词:
复合材料骨替代材料PEEK-HA生物复合材料生物相容性3T3细胞细胞增殖细胞毒性黏附率
Keywords:
composite material bone substitute materials PEEK-HA composite biocompatibility 3T3 cells proliferation cytotoxicity cell adhesion
分类号:
TB 332;Q 2-33
DOI:
10.3724/SP.J.1249.2013.04392
文献标志码:
A
摘要:
聚醚醚酮(polyetheretherketone, PEEK)与纳米羟基磷灰石(hydroxyapatite, HA)共混制备PEEK-HA生物复合材料. 通过倒置荧光显微镜观察材料对细胞形态影响,不同HA含量的PEEK-HA复合材料中活细胞数目与空白对照无明显差异. 采用噻唑蓝(3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide,MTT)染色法研究细胞增殖的作用,发现不同HA含量的PEEK-HA复合材料的质量浓度为4 mg/mL时,该生物复合材料对细胞增殖有一定促进作用;8~64 mg/mL时,对细胞增殖作用逐渐减小;64 mg/mL时,对细胞增殖作用最小,表明随着材料质量浓度增加可抑制细胞增殖. 测定PEEK-HA生物复合材料材料对3T3细胞黏附率,发现随着HA含量的增加,细胞黏附率增大,HA可以改善材料黏附性能. PEEK-HA 生物复合材料细胞毒性级别均在Ⅰ级,说明对3T3细胞无毒性.
Abstract:
On the basis of preparing polyetheretherketone-hydroxyapatite (PEEK-HA) composite materials, this paper focuses on the effect of the composite materials on 3T3 cells proliferation at different periods and under different concentrations. Based on the polycondensation of polyether-ether-ketone, the biological material was prepared by adding 10%, 20% and 30% hydroxyapatite blending into PEEK, respectively. The cell morphology 3T3 cell adhesion rate of the material was determined by cell counting with inverted fluorescence microscope. The MTT (4, 5-Dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide) method was used to study cell proliferation in various conditions. Results show that, in a concentration of 4 mg/mL,the material played a significant role in cell proliferation, and, in a concentration of 64 mg/mL, the effect of PEEK composites on cell proliferation is slight. In addition, cell adhesion rate increases with an increase of the HA content in PEEK-HA materials. The content of HA can improve cell adhesion to the composite materials. Cytotoxicity of the materials is found at levelⅠ, indicating that PEEK-HA composites are non-toxic to 3T3 cells.

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备注/Memo

备注/Memo:
Received:2013-03-19;Accepted:2013-06-05
Foundation:Guangdong Province Innovative Team Fund (9351806001000001)
Corresponding author:Professor Ni Zhuo. E-mail:royzhuoni@hotmail.com
Citation:Ni Zhuo, Liu Shide, Wang Ying, et al. Influence of 3T3 cells for PEEK-HA composite[J]. Journal of Shenzhen University Science and Engineering, 2013, 30(4): 392-397.(in Chinese)
基金项目:广东省创新团队基金资助项目(9351806001000001)
作者简介:倪卓(1963-),男(汉族),吉林省通化市人,深圳大学教授、博士生导师. E-mail:royzhuoni@hotmail.com
引文:倪卓,刘士德,王应,等. PEEK-HA生物材料对成纤维细胞3T3的影响[J]. 深圳大学学报理工版,2013,30(4):392-397.
更新日期/Last Update: 2013-07-12