[1]胡世伟,王静凤,徐雷雷,等.海参磷脂型二十碳五烯酸降血糖机制研究[J].深圳大学学报理工版,2013,30(No.4(331-440)):349-355.[doi:10.3724/SP.J.1249.2013.04349]
　Hu Shiwei,Wang Jingfeng,Xu Leilei,et al.Mechanism of eicosapentaenoic acid-enriched phosphatidylcholine isolated from sea cucumber on anti-hyperglycemia[J].Journal of Shenzhen University Science and Engineering,2013,30(No.4(331-440)):349-355.[doi:10.3724/SP.J.1249.2013.04349]
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海参磷脂型二十碳五烯酸降血糖机制研究()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第30卷
期数:
2013年No.4(331-440)
页码:
349-355
栏目:
生物工程
出版日期:
2013-07-12

文章信息/Info

Title:
Mechanism of eicosapentaenoic acid-enriched phosphatidylcholine isolated from sea cucumber on anti-hyperglycemia
文章编号:
20130402
作者:
胡世伟; 王静凤; 徐雷雷; 柳东; 楼乔明; 龙腾腾; 薛长湖
中国海洋大学食品科学与工程学院，山东青岛 266003
Author(s):
Hu Shiwei;  Wang Jingfeng;  Xu Leilei;  Liu Dong;  Lou Qiaoming;  Long Tengteng;  and Xue Changhu
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, Shandong Province, P. R. China
关键词:
糖尿病; 胰岛素; 信号转导; 糖原合成激酶-3β; 蛋白激酶B; 磷脂型二十碳五烯酸; 海参
Keywords:
diabetes;  insulin;  signal transduction;  glycogen synthase kinase-3β;  protein kinase B;  eicosapentaenoic acid-enriched phosphatidylcholine;  sea cucumber
分类号:
R 285.5
DOI:
10.3724/SP.J.1249.2013.04349
文献标志码:
A
摘要:
采用腹腔注射链尿佐菌素的方法建立糖尿病大鼠模型，经海参磷脂型二十碳五烯酸（eicosapentaenoic acid phosphatidylcholine, EPA-PC）灌胃8 周后，检测其对空腹血糖、口服葡萄糖耐量、血清胰岛素和肝糖原含量的影响，探讨其降糖机制．结果表明，海参EPA-PC能显著降低糖尿病大鼠空腹血糖水平(P<0.05)， 改善口服葡萄糖耐量（P<0.05）， 促进空腹血清胰岛素的分泌(P<0.01)， 增加肝糖原含量(P<0.01)． 进一步采用聚合酶链反应（reverse transcription polymerase chain reaction，RT-PCR）和Western blotting技术检测了海参EPA-PC对大鼠糖原合成相关基因表达的影响．RT-PCR结果显示，海参EPA-PC能明显提高IR、IRS-2、PI3K、Akt和GS等基因mRNA的表达水平（P<0.01， P<0.05）， 抑制GSK-3βmRNA的表达（P<0.05）． Western blotting检测结果表明，海参EPA-PC能显著促进IRS-2、PI3K和Akt蛋白的表达（P<0.01）， 并抑制GSK-3β蛋白的表达（P<0.05）． 提示海参EPA-PC具有显著的降血糖作用，其作用机制与激活胰岛素介导的肝脏Akt/GSK-3β信号转导通路，促进糖尿病大鼠肝糖原合成有关．
Abstract:
The diabetic rat model was established through intraperitoneal injections of STZ. Eight weeks after treatment with eicosapentaenoic acid-enriched phosphatidylcholine (EPA-PC), the effects of EPA-PC on the fasting serum glucose level, oral glucose tolerance, serum insulin level, and hepatic glycogen content in rats were analyzed, and the mechanism was investigated. Results showed that EPA-PC could significantly decrease the fasting serum glucose level (P<0.05), improve oral glucose tolerance (P<0.05), and increase serum insulin level (P<0.01) and hepatic glycogen content (P<0.01). The effects of EPA-PC on glycogen-synthesis-related genes were further investigated through reverse transcription polymerase chain reactions (RT-PCR) or Western blotting. Results showed that the transcription of IR, IRS-2 , PI3K, Akt and GS was remarkably increased by EPA-PC (P<0.01, P<0.05), while GSK-3β was significantly decreased (P<0.05). The protein expression of IRS-2, PI3K and Akt was significantly increased (P<0.01), and protein expression of GSK-3β was decreased (P<0.05). These results indicate that EPA-PC exhibit a significant hypoglycemic effect, which is related to activating the Akt/GSK-3β signal transduction pathway mediated by insulin in the liver tissues, and promoting hepatic glycogen synthesis in diabetic rats.

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相似文献/References:

[1]刘士德,张建华,余少文,等.人胰岛素和胰岛素原单克隆抗体制备及分析[J].深圳大学学报理工版,2005,22(4):340.
　LIU Shi-de,ZHANG Jian-hua,YU Shao-wen,et al.The preparation and speciality analysis of monoclonal antibody to insulin and pro-insulin[J].Journal of Shenzhen University Science and Engineering,2005,22(No.4(331-440)):340.
[2]王静凤,傅佳,徐雷雷,等.日本刺参降血糖及对胰岛素信号通路的影响[J].深圳大学学报理工版,2011,28(No.2(095-188)):172.
　WANG Jing-feng,FU Jia,XU Lei-lei,et al.Involvement of the PI3K/Akt pathway in the hypoglycemic effects of sea cucumber Apostichopus japonicas in diabetic rats[J].Journal of Shenzhen University Science and Engineering,2011,28(No.4(331-440)):172.

备注/Memo

备注/Memo:
Received：2013-01-03；Accepted：2013-06-09
Foundation：Public Science and Technology Research Funds Projects of Ocean of China (201105029); Independent Innovation Foundation of Shandong Province(2012CX80201)
Corresponding author：Professor Xue Changhu. E-mail:xuech@ouc.edu.cn
Citation：Hu Shiwei, Wang Jingfeng, Xu Leilei, et al. Mechanism of eicosapentaenoic acid-enriched phosphatidylcholine isolated from sea cucumber on anti-hyperglycemia effects［J］. Journal of Shenzhen University Science and Engineering, 2013, 30(4): 349-355.（in Chinese）
基金项目：海洋公益性行业科研专项资金资助项目(201105029)；山东省自主创新专项资助项目(2012CX80201)
作者简介：胡世伟(1984-)，男（汉族），山东省潍坊市人，中国海洋大学博士研究生. E-mail:hushiweihai@163.com
引文：胡世伟，王静凤，徐雷雷，等. 海参磷脂型二十碳五烯酸降血糖机制研究［J］. 深圳大学学报理工版，2013，30（4）：349-355.
更新日期/Last Update: 2013-07-11