[1]胡世伟,王静凤,徐雷雷,等.海参磷脂型二十碳五烯酸降血糖机制研究[J].深圳大学学报理工版,2013,30(No.4(331-440)):349-355.[doi:10.3724/SP.J.1249.2013.04349]
 Hu Shiwei,Wang Jingfeng,Xu Leilei,et al.Mechanism of eicosapentaenoic acid-enriched phosphatidylcholine isolated from sea cucumber on anti-hyperglycemia[J].Journal of Shenzhen University Science and Engineering,2013,30(No.4(331-440)):349-355.[doi:10.3724/SP.J.1249.2013.04349]
点击复制

海参磷脂型二十碳五烯酸降血糖机制研究()
分享到:

《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第30卷
期数:
2013年No.4(331-440)
页码:
349-355
栏目:
生物工程
出版日期:
2013-07-12

文章信息/Info

Title:
Mechanism of eicosapentaenoic acid-enriched phosphatidylcholine isolated from sea cucumber on anti-hyperglycemia
文章编号:
20130402
作者:
胡世伟王静凤徐雷雷柳东楼乔明龙腾腾薛长湖
中国海洋大学食品科学与工程学院,山东青岛 266003
Author(s):
Hu Shiwei Wang Jingfeng Xu Leilei Liu Dong Lou Qiaoming Long Tengteng and Xue Changhu
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, Shandong Province, P. R. China
关键词:
糖尿病胰岛素信号转导糖原合成激酶-3β蛋白激酶B磷脂型二十碳五烯酸海参
Keywords:
diabetes insulin signal transduction glycogen synthase kinase-3β protein kinase B eicosapentaenoic acid-enriched phosphatidylcholine sea cucumber
分类号:
R 285.5
DOI:
10.3724/SP.J.1249.2013.04349
文献标志码:
A
摘要:
采用腹腔注射链尿佐菌素的方法建立糖尿病大鼠模型,经海参磷脂型二十碳五烯酸(eicosapentaenoic acid phosphatidylcholine, EPA-PC)灌胃8 周后,检测其对空腹血糖、口服葡萄糖耐量、血清胰岛素和肝糖原含量的影响,探讨其降糖机制.结果表明,海参EPA-PC能显著降低糖尿病大鼠空腹血糖水平(P<0.05), 改善口服葡萄糖耐量(P<0.05), 促进空腹血清胰岛素的分泌(P<0.01), 增加肝糖原含量(P<0.01). 进一步采用聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)和Western blotting技术检测了海参EPA-PC对大鼠糖原合成相关基因表达的影响.RT-PCR结果显示,海参EPA-PC能明显提高IR、IRS-2、PI3K、Akt和GS等基因mRNA的表达水平(P<0.01, P<0.05), 抑制GSK-3βmRNA的表达(P<0.05). Western blotting检测结果表明,海参EPA-PC能显著促进IRS-2、PI3K和Akt蛋白的表达(P<0.01), 并抑制GSK-3β蛋白的表达(P<0.05). 提示海参EPA-PC具有显著的降血糖作用,其作用机制与激活胰岛素介导的肝脏Akt/GSK-3β信号转导通路,促进糖尿病大鼠肝糖原合成有关.
Abstract:
The diabetic rat model was established through intraperitoneal injections of STZ. Eight weeks after treatment with eicosapentaenoic acid-enriched phosphatidylcholine (EPA-PC), the effects of EPA-PC on the fasting serum glucose level, oral glucose tolerance, serum insulin level, and hepatic glycogen content in rats were analyzed, and the mechanism was investigated. Results showed that EPA-PC could significantly decrease the fasting serum glucose level (P<0.05), improve oral glucose tolerance (P<0.05), and increase serum insulin level (P<0.01) and hepatic glycogen content (P<0.01). The effects of EPA-PC on glycogen-synthesis-related genes were further investigated through reverse transcription polymerase chain reactions (RT-PCR) or Western blotting. Results showed that the transcription of IR, IRS-2 , PI3K, Akt and GS was remarkably increased by EPA-PC (P<0.01, P<0.05), while GSK-3β was significantly decreased (P<0.05). The protein expression of IRS-2, PI3K and Akt was significantly increased (P<0.01), and protein expression of GSK-3β was decreased (P<0.05). These results indicate that EPA-PC exhibit a significant hypoglycemic effect, which is related to activating the Akt/GSK-3β signal transduction pathway mediated by insulin in the liver tissues, and promoting hepatic glycogen synthesis in diabetic rats.

参考文献/References:

[1] Krssak M, Brehm A, Bernroider E, et al. Alterations in postprandial hepatic glycogen metabolism in type 2 diabetes[J]. Diabetes, 2004, 53(12): 3048-3056.
[2] Wang Jingfeng, Fu Jia, Xu Leilei, et al. Involvement of the PI3K/Akt pathway in the hypoglycemic effects of sea cucumber Apostichopus japonicas in diabetic rats[J]. Journal of Shenzhen University Science and Engineering, 2011, 28(2): 172-178. (in Chinese)
王静凤, 傅佳, 徐雷雷, 等.日本刺参降血糖及对胰岛素信号通路的影响[J].深圳大学学报理工版,2011,28(2):172-178.
[3] Lou Qiaoming, Wang Yuming, Liu Xiaofang, et al. Lipid profile and fatty acid compositions in body wall of Apostichopus japonicus(selenka)[J]. Journal of Food Biochemistry, 2012, 36(3): 317-321.
[4] Xu Leilei, Wang Jingfeng, Liu Dong, et al. Effects of sea cucumber eicosapentaenoic acid rich in phosphatidylcholine on rat model of diabetes mellitus[J]. Chinese Pharmacological Bulletin, 2012, 28(10): 1398-1402. (in Chinese)
徐雷雷, 王静凤, 柳东, 等. 海参磷脂型二十碳五烯酸对糖尿病大鼠血葡萄糖耐量的影响[J]. 中国药理学通报, 2012, 28(10): 1398-1402.
[5] Bhuvaneswari S, Anuradha C V. Astaxanthin prevents loss of insulin signaling and improves glucose metabolism in liver of insulin resistant mice[J]. Canadian Journal of Physiology and Pharmacology, 2012, 90(11): 1544-1552.
[6] Fisher S J, Kahn C R. Insulin signaling is required for insulin’s direct and indirect action on hepatic glucose production[J]. Journal of Clinical Investigation, 2003, 111(4): 463-468.
[7] Kubota N, Kubota T, Itoh S, et al. Dynamic functional relay between insulin receptor substrate 1 and 2 in hepatic insulin signaling during fasting and feeding[J]. Cell Metabolism, 2008, 8(1): 49-64.
[8] Lai Y C, Liu Y, Jacobs R, et al. A novel PKB/Akt inhibitor, MK-2206, effectively inhibits insulin-stimulated glucose metabolism and protein synthesis in isolated rat skeletal muscle[J]. Biochemical Journal, 2012, 447(1): 137-147.
[9] Xie Xi, Li Wenyuan, Lan Tian, et al. Berberine ameliorates hyperglycemia in alloxan-induced diabetic C57BL/6 mice through activation of Akt signaling pathway[J]. Endocrine Journal, 2011, 58 (9): 761-768.
[10] Hoene M, Lehmann R, Hennige A M, et al.Acute regulation of metabolic genes and insulin receptor substrates in the liver of mice by one single bout of treadmill exercise[J].Journal of Physiology, 2009, 587(1): 241-252.
[11] Haseena B H K, Kaladevi S V, Shanthi P, et al. Ameliorating effect of Semecarpus anacardium Linn. nut milk extract on altered glucose metabolism in high fat diet STZ induced type 2 diabetic rats[J]. Asian Pacific Journal of Tropical Disease, 2012, 5(12): 956-961.
[12] Fang Y, Studer E, Mitchell C, et al. Conjugated bile acids regulate hepatocyte glycogen synthase activity in vitro and in vivo via Gαi signaling[J]. Molecular Pharmacology, 2007, 71(14): 1122-1128.

相似文献/References:

[1]刘士德,张建华,余少文,等.人胰岛素和胰岛素原单克隆抗体制备及分析[J].深圳大学学报理工版,2005,22(4):340.
 LIU Shi-de,ZHANG Jian-hua,YU Shao-wen,et al.The preparation and speciality analysis of monoclonal antibody to insulin and pro-insulin[J].Journal of Shenzhen University Science and Engineering,2005,22(No.4(331-440)):340.
[2]王静凤,傅佳,徐雷雷,等.日本刺参降血糖及对胰岛素信号通路的影响[J].深圳大学学报理工版,2011,28(No.2(095-188)):172.
 WANG Jing-feng,FU Jia,XU Lei-lei,et al.Involvement of the PI3K/Akt pathway in the hypoglycemic effects of sea cucumber Apostichopus japonicas in diabetic rats[J].Journal of Shenzhen University Science and Engineering,2011,28(No.4(331-440)):172.

备注/Memo

备注/Memo:
Received:2013-01-03;Accepted:2013-06-09
Foundation:Public Science and Technology Research Funds Projects of Ocean of China (201105029); Independent Innovation Foundation of Shandong Province(2012CX80201)
Corresponding author:Professor Xue Changhu. E-mail:xuech@ouc.edu.cn
Citation:Hu Shiwei, Wang Jingfeng, Xu Leilei, et al. Mechanism of eicosapentaenoic acid-enriched phosphatidylcholine isolated from sea cucumber on anti-hyperglycemia effects[J]. Journal of Shenzhen University Science and Engineering, 2013, 30(4): 349-355.(in Chinese)
基金项目:海洋公益性行业科研专项资金资助项目(201105029);山东省自主创新专项资助项目(2012CX80201)
作者简介:胡世伟(1984-),男(汉族),山东省潍坊市人,中国海洋大学博士研究生. E-mail:hushiweihai@163.com
引文:胡世伟,王静凤,徐雷雷,等. 海参磷脂型二十碳五烯酸降血糖机制研究[J]. 深圳大学学报理工版,2013,30(4):349-355.
更新日期/Last Update: 2013-07-11