Mo Beixin,Huang Biao,Liu Li,et al.Utilization of stored mRNA during the germination of soybean radicle[J].Journal of Shenzhen University Science and Engineering,2013,30(No.1(001-110)):84-90.[doi:10.3724/SP.J.1249.2013.01084]





Utilization of stored mRNA during the germination of soybean radicle
1)深圳大学生命科学学院,深圳市微生物基因工程重点实验室,深圳 518060
2)深圳大学生命科学学院,深圳市海洋生物技术与生态环境重点实验室,深圳 518060
Mo Beixin1 Huang Biao1 Liu Li2 Ye Hao1 and Xu Xiaofeng2
1)College of Life Science, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University, Shenzhen 518060, P.R.China
2) College of Life Sciences, Shenzhen Key Laboratory of Marine Biological Resources and Ecological Environment, Shenzhen University, Shenzhen 518060, P.R.China
plant cell physiology storage mRNA seed germiantion soybean radicle transcription inhibitor α-amanitin translation inhibitor cycloheximide real-time PCR sucrose density gradient ultracentrifugation
Q 942.5;Q 256
Although intensive studies have been conducted in seed germination, the exact requirements for germination is still poorly understood, particularly in terms of de novo RNA and protein syntheses. To find out if seeds can complete germination relying solely on stored mRNAs or stored proteins, transcription inhibitor α-amanitin and translation inhibitor cycloheximide were used to inhibit the synthesis of new mRNAs and new proteins of soybean radicles respectively. Germination of soybean radicles were observed after these treatments. Results show that, under the conditions of inhibition of transcription, soybean radicles are able to complete germination, but seedling growth stops. Under the conditions of the inhibition of translation, soybean radicles cannot complete germination. These results indicate that soybean radicle germination can rely on the mRNAs synthesized during seed development and stored in the dry seeds. The development of the seedlings cannot totally rely on stored mRNAs: newly synthesized mRNAs are required for this process. Also newly synthesized proteins are needed for soybean radicle germination. Fractionation of soybean radicle cell components by ultracentrifugation and quantification of mRNAs in different cell fractions by real-time PCR finds that mRNAs exist mainly in non-polysome structures in the dry seed while they exist mainly in the polysome after 4 hours of imbibition.This suggests that mRNAs are mainly in the state of storage in dry seeds. After imbibition, mRNAs move to polysome in the state of translation. Polysome profiles in different times of imbibition also reflect the movement of mRNAs during the germination process.


[1] Bewley J D.Seed germination and dormancy[J]. Plant Cell, 1997, 9(7): 1055-1066.
[2] Almoguera C, Jordano J. Developmental and environmental concurrent expression of sunflower dry-seed-stored low-molecuLar-weight heat-shock protein and Lea mRNAs[J]. Plant Molecular Biology, 1992, 19(5): 781-792.
[3] Ishibashi N, Yamauchi D, Minamikawa T. Stored mRNA in cotyledons of Vigna unguicuLata seeds: nucleotide sequence of cloned cDNA for a stored mRNA and induction of its synthesis by precocious germination[J]. Plant Molecular Biology, 1990, 15(1): 59-64.
[4] Ku Ligowski J M, Ferrand E Chenou. Stored mRNA in early embryos of a fern Marsilea vestita: a paternal and maternal origin[J]. Molecular Reproduction and Development, 1991, 30(1): 27-33.
[5] Rajjou L, Gallardo K, Debeaujon I, et al.The effect of alpha-amanitin on the Arabidopsis seed proteome highlights the distinct roles of stored and neosynthesized mRNAs during germination[J].Plant Physiol, 2004, 134(4): 1598-1613.
[6] Dure L, Waters L. Long-lived messenger RNA: evidence from cotton seed germination[J].Science, 1965, 147(3656): 410-412.
[7] Nakabayashi K, Okamoto M, Koshiba T, et al.Genome-wide profiling of stored mRNA in Arabidopsis thaliana seed germination: epigenetic and genetic reguLation of transcription in seed[J]. The Plant Journal, 2005, 41(5): 697-709.
[8] Kimura M, Nambara E. Stored and neosynthesized mRNA in Arabidopsis seeds: effects of cycloheximide and controlled deterioration treatment on the resumption of transcription during imbibition[J]. Plant Molecular Biology, 2010, 73(1-2): 119-129.
[9] Okamoto M, Tatematsu K, Matsui A, et al. Genome-wide analysis of endogenous abscisic acid-mediated transcription in dry and imbibed seeds of Arabidopsis using tiling arrays[J]. The Plant Journal, 2010, 62(1): 39-51.
[10] Howell K A, Narsai R, Carroll A, et al. Mapping metabolic and transcript temporal switches during germination in rice highlights specific transcription factors and the role of RNA instability in the germination process[J]. Plant Physiology, 2009, 149(2): 961-980.
[11] Sano N, Permana H,Kumada R,et al.Proteomic analysis of embryonic proteins synthesized from long-lived mRNAs during germination of rice seeds[J]. Plant Cell Physiol, 2012, 53(4): 687-698.
[12] Sreenivasu Lu N, Borisjuk L, Junker B H, et al. Barley grain development toward an integrative view[J]. International Review of Cell and Molecular Biology, 2010, 281: 49-89.
[13] Asakura T, Tamura T, Terauchi K, et al. Global gene expression profiles in developing soybean seeds[J]. Plant Physiol Biochem, 2012, 52: 147-153.
[14] Brengues M,Teixeira D,Parker R.Movement of eukaryotic mRNAs between polysomes and cytoplasmic processing bodies[J]. Science, 2005, 310(5747): 486-489.
[15] Floris M,Mahgoub H,Lanet E,et al.Post-transcriptional regulation of gene expression in plants during abiotic stress[J]. International Journal of Molecular Sciences, 2009, 10(7): 3168-3185.
[16] Xu J, Chua N H. Processing bodies and plant development[J]. Current Opinion in Plant Biology, 2011, 14(1): 88-93.
[17] Weber C, Nover L, Fauth M. Plant stress granuLes and mRNA processing bodies are distinct from heat stress granules[J]. The Plant Journal, 2008, 56(4): 517-530.
[18] Saroj K Pramanik, Joan E Krochko, Bewley J Derek. Distribution of cytosolic mRNAs between polysomal and ribonucleoprotein complex fractions in alfalfa embryos[J]. Plant Physiology, 1992, 99: 1590-1596.
[19] Mo Beixin, Ye Hao, Ou Zhonghua, et al. Stress-induced processing body formation in tobacco suspension cells[J]. Journal of Shenzhen University Science and Engineering, 2012, 29(1): 80-84.(in Chinese)
莫蓓莘,叶浩,欧忠华,逆境诱导烟草悬浮细胞内处理小体的形成[J]. 深圳大学学报理工版,2012,29(1): 80-84.
[20] Liu Yike,Sun Hongbo,Jian Bo,et al. Two efficient improved methods for isolation of high quality total RNA from soybean[J]. Journal of Northwest Sci-Tech University of Agriculture and Forestry, 2006, 34(12):83-86.
[21] Del prete M J, Rolando Vernal, Helmut Dolznig, et al. Isolation of polysome-bound mRNA from solid tissues amenable for RT-PCR and profiling experiments[J]. RNA, 2007, 13(3): 414-421.
[22] Ashe M P, De Long S K, Sachs A B, et al. Glucose depletion rapidly inhibits translation initiation in yeast[J]. Molecular Biology of the Cell, 2000, 11(3): 833-834.


Foundation:National Natural Science Foundation of China (30970265)
Corresponding author:Professor Mo Beixin. E-mail: bmo@szu.edu.cn
Citation:Mo Beixin, Huang Biao, Liu Li, et al. Utilization of stored mRNA during the germination of soybean radicle [J]. Journal of Shenzhen University Science and Engineering, 2013, 30(1): 84-90.(in Chinese)
作者简介:莫蓓莘(1966-),女(汉族),江西省修水县人,深圳大学教授. E-mail: bmo@szu.edu.cn
引文:莫蓓莘,黄彪,刘丽,等.储存mRNA在大豆胚根萌发过程的作用[J]. 深圳大学学报理工版,2013,30(1):84-90.
更新日期/Last Update: 2013-01-20