[1]莫蓓莘,黄彪,刘丽,等.储存mRNA在大豆胚根萌发过程的作用[J].深圳大学学报理工版,2013,30(No.1(001-110)):84-90.[doi:10.3724/SP.J.1249.2013.01084]
　Mo Beixin,Huang Biao,Liu Li,et al.Utilization of stored mRNA during the germination of soybean radicle[J].Journal of Shenzhen University Science and Engineering,2013,30(No.1(001-110)):84-90.[doi:10.3724/SP.J.1249.2013.01084]
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储存mRNA在大豆胚根萌发过程的作用()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第30卷
期数:
2013年No.1(001-110)
页码:
84-90
栏目:
生物工程
出版日期:
2013-01-31

文章信息/Info

Title:
Utilization of stored mRNA during the germination of soybean radicle
作者:
莫蓓莘1; 黄彪1; 刘丽2; 叶浩1; 徐晓峰2
1）深圳大学生命科学学院，深圳市微生物基因工程重点实验室，深圳 518060
2）深圳大学生命科学学院，深圳市海洋生物技术与生态环境重点实验室，深圳 518060
Author(s):
Mo Beixin1;  Huang Biao1;  Liu Li2;  Ye Hao1;  and Xu Xiaofeng2
1)College of Life Science, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University, Shenzhen 518060, P.R.China
2) College of Life Sciences, Shenzhen Key Laboratory of Marine Biological Resources and Ecological Environment, Shenzhen University, Shenzhen 518060, P.R.China
关键词:
翻译抑制剂; 放线菌酮; 实时定量聚合酶链式反应; 蔗糖密度梯度超速离心
Keywords:
plant cell physiology;  storage mRNA;  seed germiantion;  soybean radicle;  transcription inhibitor;  α-amanitin;  translation inhibitor;  cycloheximide;  real-time PCR;  sucrose density gradient ultracentrifugation
分类号:
Q 942.5；Q 256
DOI:
10.3724/SP.J.1249.2013.01084
文献标志码:
A
摘要:
为研究种子是否能完全依靠储存的mRNA或储存的蛋白质完成萌发过程，分别利用转录抑制剂α-鹅膏蕈碱（α-amanitin）和制剂放线菌酮处理大豆胚根，抑制新的mRNA或新的蛋白质的合成，观察大豆胚根的萌发情况．结果发现，在转录抑制的条件下，大豆胚根能够完成萌发，种子萌发后，种苗生长停止；在翻译被抑制的条件下，大豆胚根不能完成萌发．说明大豆胚根萌发能够依赖发育过程合成并贮存在干种子中的mRNA；但种苗的发育不能完全依赖储存的RNA，需要新合成的mRNA参与.大豆胚根不能依赖储存蛋白质完成萌发过程．对大豆胚根细胞内容物进行超速离心并对不同组分中mRNA含量进行定量分析发现，干种子中的mRNA主要存在于多核糖体之外的细胞结构中，而在萌发过程中，mRNA主要存在于多核糖体中．这表明干种子中的mRNA主要以储存状态存在于mRNA的储存结构中，萌发过程大部分mRNA从储存结构中转移到多核糖体中变为活跃的翻译状态．不同萌发时期多核糖体的特征曲线也体现mRNA在细胞内存在位置的这种变化．
Abstract:
Although intensive studies have been conducted in seed germination, the exact requirements for germination is still poorly understood, particularly in terms of de novo RNA and protein syntheses. To find out if seeds can complete germination relying solely on stored mRNAs or stored proteins, transcription inhibitor α-amanitin and translation inhibitor cycloheximide were used to inhibit the synthesis of new mRNAs and new proteins of soybean radicles respectively. Germination of soybean radicles were observed after these treatments. Results show that, under the conditions of inhibition of transcription, soybean radicles are able to complete germination, but seedling growth stops. Under the conditions of the inhibition of translation, soybean radicles cannot complete germination. These results indicate that soybean radicle germination can rely on the mRNAs synthesized during seed development and stored in the dry seeds. The development of the seedlings cannot totally rely on stored mRNAs: newly synthesized mRNAs are required for this process. Also newly synthesized proteins are needed for soybean radicle germination. Fractionation of soybean radicle cell components by ultracentrifugation and quantification of mRNAs in different cell fractions by real-time PCR finds that mRNAs exist mainly in non-polysome structures in the dry seed while they exist mainly in the polysome after 4 hours of imbibition.This suggests that mRNAs are mainly in the state of storage in dry seeds. After imbibition, mRNAs move to polysome in the state of translation. Polysome profiles in different times of imbibition also reflect the movement of mRNAs during the germination process.

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备注/Memo

备注/Memo:
Received：2012-11-12；Accepted：2012-12-23
Foundation：National Natural Science Foundation of China (30970265)
Corresponding author：Professor Mo Beixin. E-mail: bmo@szu.edu.cn
Citation：Mo Beixin, Huang Biao, Liu Li, et al. Utilization of stored mRNA during the germination of soybean radicle ［J］. Journal of Shenzhen University Science and Engineering, 2013, 30(1): 84-90.（in Chinese）
基金项目：国家自然科学基金资助项目（30970265）；深圳大学基础研究基金资助项目(00035222)
作者简介：莫蓓莘（1966-），女（汉族），江西省修水县人，深圳大学教授. E-mail: bmo@szu.edu.cn
引文：莫蓓莘，黄彪，刘丽，等.储存mRNA在大豆胚根萌发过程的作用［J］. 深圳大学学报理工版，2013，30（1）：84-90.
更新日期/Last Update: 2013-01-20