BAO Mu-tai,WANG Li-na,LI Yi-ming,et al.Degradation characteristics research of a petroleum-degrading bacteria D2-1[J].Journal of Shenzhen University Science and Engineering,2012,29(No.6(471-580)):515-520.[doi:10.3724/SP.J.1249.2012.06515]





Degradation characteristics research of a petroleum-degrading bacteria D2-1
1)海洋化学理论与工程技术教育部重点实验室,中国海洋大学,山东 青岛 266100;
2)国家海洋局海洋溢油鉴别与损害评估技术重点实验室,国家海洋局北海环境监测中心,山东 青岛 266033
BAO Mu-tai1 WANG Li-na1 LI Yi-ming1 SUN Pei-yan2 YANG Xiao-fei2 and CAO Li-xin2
1) Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education,Ocean University of China, Qingdao 266100, Shandong Province, P.R.China
2) Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology, North China Sea Environmental Monitoring Center of State Oceanic Administration, Qingdao 266033, Shandong Province, P.R.China
环境微生物学 石油污染 生物降解 石油烃降解菌 生物修复 寡养食单胞菌 去除率
environmental microbiology oil pollution biodegradation petroleum-degrading bacteria bioremediation Stenotrophomonas sp. removal rate
X 172;X 55
从山东东营受原油污染土壤中筛选得到一组高效石油烃降解菌群,对降解率较高的菌株D2-1进行烃降解性能评价. 通过生理生化鉴定及16S rDNA基因序列分析,D2-1被确定为寡养食单胞菌(Stenotrophomonas sp.). 优化实验确定了菌株D2-1降解原油的最佳条件为:底物质量浓度为2 g/L,pH值为8.0,温度为25 ℃,NaCl质量浓度为30 g/L. 在最佳降解条件下,该菌原油去除率超过50%. 由微生物降解前后烃组分的气相色谱分析可知,菌株D2-1对原油中的C9~C38都能进行有效降解,正构烷烃的去除率最高. 菌株D2-1对原油的降解符合一级反应动力学过程.
A group of petroleum-degrading bacteria were isolated from oil-polluted area in Dongying Port in China. One strain, D2-1, with the highest crude oil removal rate was adopted in this study. The strain was identified as Stenotrophomonas sp. D2-1 based on morphological, biochemical and phylogenetic analyses. Optimum environmental conditions that were suitable for crude oil degradation were determined to include a pH of 8.0, a temperature of 25 ℃, and a NaCl solution with a concentration of 30 g/L. Under the optimum conditions, the oil degradation rate was over 50%. Results of GC-FID analysis show that strain D2-1 can degrade normal alkanes from C9 to C38 obviously. It also indicates that strain D2-1 has some potential in the bioremediation process in the field. The exploration of the hydrocarbon degradation shows that the degradation of strain D2-1 is in line with the first order reaction kinetics.


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Foundation:Program for New Century Excellent Talents in University(NCET-11-0464);Development Program of Science and Technology in Shandong Province(2011GHY11522)
Corresponding author:Professor BAO Mu-tai. E-mail: mtbao@ouc.edu.cn
Citation:BAO Mu-tai, WANG Li-na, LI Yi-ming, et al. Degradation characteristics research of a petroleum-degrading bacteria D2-1[J]. Journal of Shenzhen University Science and Engineering, 2012, 29(6): 515-520.(in Chinese)
作者简介:包木太(1971-), 男(汉族), 山东省临沂市人, 中国海洋大学教授、博士生导师. E-mail: mtbao@ouc.edu.cn
引文:包木太,王丽娜,李一鸣,等. 一株石油烃降解菌D2-1解烃性能研究[J]. 深圳大学学报理工版,2012,29(6):515-520.
更新日期/Last Update: 2012-11-30