[1]李英华,李海波,孙铁珩,等.地下渗滤系统酶活性分布及对脱氮效果的影响[J].深圳大学学报理工版,2012,29(No.5(377-470)):461-465.[doi:10.3724/SP.J.1249.2012.05461]
　LI Ying-hua,LI Hai-bo,and SUN Tie-heng.Spatial distribution of soil enzyme activities and influence on nitrogen removal in subsurface wastewater infiltration system[J].Journal of Shenzhen University Science and Engineering,2012,29(No.5(377-470)):461-465.[doi:10.3724/SP.J.1249.2012.05461]
点击复制

地下渗滤系统酶活性分布及对脱氮效果的影响()
分享到：

《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第29卷
期数:
2012年No.5(377-470)
页码:
461-465
栏目:
环境与能源
出版日期:
2012-09-21

文章信息/Info

Title:
Spatial distribution of soil enzyme activities and influence on nitrogen removal in subsurface wastewater infiltration system
作者:
李英华1;  李海波1;  孙铁珩1;  2
1) 沈阳大学区域污染环境生态修复教育部重点实验室, 沈阳110044
2) 中国科学院沈阳应用生态研究所, 沈阳 110016
Author(s):
LI Ying-hua1;  LI Hai-bo1;  and SUN Tie-heng1; 2
1）Key Laboratory of Regional Environment and Eco-remediation, Ministry of Education, Shenyang University, Shenyang 110044, P. R. China
2) Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. China
关键词:
环境工程; 地下渗滤;  酶活性;  空间分布;  脱氮;  污水处理
Keywords:
environmental engineering; subsurface wastewater infiltration system;  enzyme activity;  spatial distribution;  denitrification;  wastewater treatment
分类号:
X 703.1
DOI:
10.3724/SP.J.1249.2012.05461
文献标志码:
A
摘要:
利用污水地下渗滤中试系统, 考察脲酶、硝酸还原酶和亚硝酸还原酶活性的空间分布规律, 分析酶活性对脱氮效果的影响. 结果表明，在靠近散水管位置, 脲酶活性强, 且随进水氨氮含量及温度的升高而增强; 硝酸还原酶活性分布主要受基质深度的影响, 随着基质深度的增大而增强; 亚硝酸还原酶活性随温度的升高而增强. 不同深度的脲酶活性与总氮去除率之间均呈显著正相关; 硝酸还原酶活性与氨氮和总氮去除率之间的相关性不显著; 60~80 cm 区域内, 亚硝酸还原酶活性与总氮去除率之间存在显著正相关. 通过分析酶活性与氮去除效果的相关性, 提出可将脲酶和亚硝酸还原酶作为指示地下渗滤系统总氮脱除效果的酶学指标.
Abstract:
A set of pilot-scale subsurface wastewater infiltration system was constituted. The distribution of urease, nitrate-reductase (NAR) and nitrite-reductase (NIR) was studied. Furthermore, the influence of enzyme activities on nitrogen removal was investigated accordingly. It was discovered that the distribution of urease was affected by depth, ammonia nitrogen (NH4+-N) concentration of input and temperature. In the position near the wastewater distribution area, the urease activity was the strongest, and it was in positive correlation with NH4+-N concentration and temperature. The distribution of the NAR activity was affected more by depth than by either temperature changes or inflow characteristics, and it was in positive correlation with depth. Depth and temperature had profound influence on the distribution of NIR activity, which was in positive correlation with temperature. The Urease activity was in significant correlation with total nitrogen (TN) removal. No significant correlation was found between NAR activity and nitrogen removal. From 60 cm to 80 cm, the NIR activity was in significant correlation with TN removal. The results indicate that it is feasible to investigate urease and NIR activities as an enzyme index in determining TN removal effects on subsurface wastewater infiltration system (SWIS).

参考文献/References:

［1］ LI Ying-hua, SUN Tie-heng, LI Hai-bo, et al. Problems and solutions of subsurface infiltration system in domestic wastewater treatment［J］. Chinese Journal of Ecology, 2009, 28(7): 1415-1418.(in Chinese).
李英华, 孙铁珩, 李海波, 等. 地下渗滤系统处理生活污水的技术难点及对策［J］. 生态学杂志, 2009, 28(7): 1415-1418.
［2］ WEI Cai-jie, ZHU Qing, WU Wei-zhong, et al. Comparison of two types of multi-soil-layering treatment system filled different paddings［J］. Environmental Science, 2009, 30(6): 1860-1863.(in Chinese)
魏才倢, 朱擎, 吴为中, 等. 2种不同模块化填料组合的多级土壤渗滤系统的比较［J］. 环境科学, 2009, 30(6): 1860-1863.
［3］ Arve H, Adam M, Lasse V. A high-performance compact filter system treating domestic wastewater［J］. Ecological Engineering, 2006, 28: 374-379.
［4］ Zhang J, Huang X, Liu C X, et al. Nitrogen removal enhanced by intermittent operation in a subsurface wastewater infiltration system［J］. Ecological Engineering, 2005, 25: 419-428.
［5］ PAN Jing, ZHANG Yang, SUN Tie-heng, et al. Spatial distribution of microorganisms in subsurface wastewater infiltration and their correlation with purification of wastewater［J］. China Environmental Science, 2008, 28(7): 656-660.(in Chinese)
潘晶, 张阳, 孙铁珩, 等. 地下渗滤基质中微生物空间分布与污水净化效果［J］. 中国环境科学, 2008, 28(7): 656-660.
［6］ ZOU Yi, WANG Shu-wen, LI Ying-hua, et al. A novel substrate enhancing wastewater treatment effects and its preparation method: China, ZL200610045737.0［P］. 2007-01-03.(in Chinese)
邹轶, 王书文, 李英华, 等. 一种高效污水处理促进剂及其制备方法: 中国, ZL200610045737.0［P］. 2007-01-03.
［7］ GUAN Song-yin. Soil Enzyme and the Research Method［M］. Beijing: Agricultural Press, 1986: 294-332.(in Chinese)
关松荫. 土壤酶及其研究法［M］. 北京: 农业出版社, 1986: 294-332.
［8］ State Environmental Protection Administration of China. Method of Water and Wastewater Inspection and Analysis［M］. Beijing: China Environmental Science Press, 2002: 254-279.(in Chinese)
国家环境保护总局. 水和废水监测分析方法［M］. 北京: 环境科学出版社, 2002: 254-279.
［9］ William M H, Paul M B, Hidetoshi U, et al. Ammonia oxidation kinetics determines niche separation of nitrifying archaea and bacteria［J］. Nature, 2009, 461(15): 976-979.
［10］ Masoud T, Azadeh S. Long-term impact of municipal sewage irrigation on treated soil and black locust trees in a semi-arid suburban area of Iran［J］. Journal of Environmental Sciences, 2009, 2: 1438-1445.
［11］ Zheng S K, Li W. Effects of hydraulic loading and room temperature on performance of anaerobic/aerobic system for ammonia-ridden and phenol-rich coking effluents［J］. Desalination, 2009, 247: 362-369.
［12］ David A, Jaime N. Energy requirements for nitrification and biological nitrogen removal in engineered wetlands［J］. Ecological Engineering, 2009, 35: 184-192.
［13］ Fen X Y, Ying L. Enhancement of nitrogen removal in towery hybrid constructed wetland to treat domestic wastewater for small rural communities［J］. Ecological Engineering, 2009, 35: 1043-1050.
［14］ Kuschk P A, Wieber U, Kappelmehyer E, et al. Annual cycle of nitrogen removal by a pilot-scale subsurface horizontal flow in a constructed wetland under moderate climate［J］. Water Research, 2003, 37: 4236-4242.
［15］ Tang X, Huang X, Miklas S. Nutrient removal in wetlands during intermittent artificial aeration［J］. Environmental Engineering Science, 2008, 25: 1279-1285.
［16］ KE Xin, ZHANG Yun, LI Pei-jun, et al. Leaching remediation of a heavy metal contaminated soil using tartaric acid［J］. Journal of Shenzhen University Science and Engineering, 2009, 26(3): 25-28.(in Chinese)
可欣, 张昀, 李培军, 等. 利用酒石酸土柱淋洗法修复重金属污染土壤［J］. 深圳大学学报理工版, 2009, 26(3): 25-28.

相似文献/References:

[1]杨波,李影影,张峰振,等.铝板电絮凝去除水体中镍离子的研究[J].深圳大学学报理工版,2014,31(4):415.[doi:10.3724/SP.J.1249.2014.04415]
　Yang Bo,Li Yingying,Zhang Fengzhen,et al.The removal of Ni2+ in wastewater by electrocoagulation using aluminum electrode[J].Journal of Shenzhen University Science and Engineering,2014,31(No.5(377-470)):415.[doi:10.3724/SP.J.1249.2014.04415]
[2]苏子艺,余江,王慈,等.生活污泥制备轻质高比表面积陶粒的试验研究[J].深圳大学学报理工版,2014,31(4):374.[doi:10.3724/SP.J.1249.2014.04374]
　Su Ziyi,Yu Jiang,et al.Preparation of lightweight and high specific surface area ceramsites with sewage sludge[J].Journal of Shenzhen University Science and Engineering,2014,31(No.5(377-470)):374.[doi:10.3724/SP.J.1249.2014.04374]
[3]马睿,闫江龙,方琳,等.超临界水氧化去除污泥中化学需氧量的动力学[J].深圳大学学报理工版,2015,32(6):617.[doi:10.3724/SP.J.1249.2015.06617]
　Ma Rui,Yan Jianglong,Fang Lin,et al.Kinetics of removal of chemical oxygen demand in municipal sewage sludge by supercritical water oxidation[J].Journal of Shenzhen University Science and Engineering,2015,32(No.5(377-470)):617.[doi:10.3724/SP.J.1249.2015.06617]
[4]毕莹莹,郭进,郭玉文,等.润湿剂对PS和ABS的浮选分离效果及其作用机制[J].深圳大学学报理工版,2016,33(6):558.[doi:10.3724/SP.J.1249.2016.06558]
　Bi Yingying,Guo Jin,Guo Yuwen,et al.Effects and mechanism of wetting agents on the separation of PS and ABS by flotation[J].Journal of Shenzhen University Science and Engineering,2016,33(No.5(377-470)):558.[doi:10.3724/SP.J.1249.2016.06558]
[5]王宁,郭斌,王欣,等.微波场中催化剂对甲苯废气的氧化分解研究[J].深圳大学学报理工版,2018,35(6):582.[doi:10.3724/SP.J.1249.2018.06582]
　WANG Ning,GUO Bin,WANG Xin,et al.Oxidation decomposition of toluene waste gas by catalyst in microwave field[J].Journal of Shenzhen University Science and Engineering,2018,35(No.5(377-470)):582.[doi:10.3724/SP.J.1249.2018.06582]
[6]张晓春,丘建栋,屈新明,等.深圳市交通排放污染物浓度特征与影响因素[J].深圳大学学报理工版,2020,37(2):178.[doi:10.3724/SP.J.1249.2020.02178]
　ZHANG Xiaochun,QIU Jiandong,QU Xinming,et al.Characteristics and influencing factors of traffic pollutant emission concentration in Shenzhen City[J].Journal of Shenzhen University Science and Engineering,2020,37(No.5(377-470)):178.[doi:10.3724/SP.J.1249.2020.02178]
[7]刘安,李玉清,郭奇聪,等.深圳典型道路原位模拟雨水生物毒性评价分析[J].深圳大学学报理工版,2020,37(4):355.[doi:10.3724/SP.J.1249.2020.04355]
　LIU An,LI Yuqing,GUO Qicong,et al.Biotoxicity assessment of in-situ simulated stormwater runoff on typical urban roads in Shenzhen[J].Journal of Shenzhen University Science and Engineering,2020,37(No.5(377-470)):355.[doi:10.3724/SP.J.1249.2020.04355]

备注/Memo

备注/Memo:
Received：2010-12-27；Revised：2012-03-20；Accepted：2012-05-30
Foundation：National Natural Science Foundation of China ( 51108275, 50908151)
Corresponding author：Professor LI Hai-bo. E-mail: iamlhb@126.com
Citation：LI Ying-hua, LI Hai-bo, SUN Tie-heng. Spatial distribution of soil enzyme activities and influence on nitrogen removal in subsurface wastewater infiltration system ［J］. Journal of Shenzhen University Science and Engineering, 2012, 29(5): 461-465.（in Chinese）
基金项目：国家自然科学基金资助项目(51108275, 50908151)
作者简介：李英华 (1979-), 女 (汉族), 辽宁省沈阳市人，沈阳大学副教授、博士. E-mail: liyinghua1028@126.com
引文：李英华, 李海波, 孙铁珩. 地下渗滤系统酶活性分布及对脱氮效果的影响［J］. 深圳大学学报理工版，2012，29（5）：461-465.
更新日期/Last Update: 2012-09-26