[1]莫蓓莘,叶浩,欧忠华,等.逆境诱导烟草悬浮细胞内处理小体的形成[J].深圳大学学报理工版,2012,29(No.1(001-094)):80-84.[doi:10.3724/SP.J.1249.2012.01080]
　MO Bei-xin,YE Hao,OU Zhong-hua,et al.Stress-induced processing body formation in tobacco suspension cells[J].Journal of Shenzhen University Science and Engineering,2012,29(No.1(001-094)):80-84.[doi:10.3724/SP.J.1249.2012.01080]
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逆境诱导烟草悬浮细胞内处理小体的形成()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第29卷
期数:
2012年No.1(001-094)
页码:
80-84
栏目:
生物工程
出版日期:
2012-01-06

文章信息/Info

Title:
Stress-induced processing body formation in tobacco suspension cells
作者:
莫蓓莘1; 叶浩1; 欧忠华1; 刘丽1; 黄彪1; 朱双琳1; 2
1) 深圳大学生命科学学院，深圳 518060
2) 麦克马斯特大学健康科学系，加拿大 哈密尔顿 L8S4L8
Author(s):
MO Bei-xin1;  YE Hao1;  OU Zhong-hua1;  LIU Li1;  HUANG Biao1;  and ZHU Shuang-lin1; 2
1) College of Life Science, Shenzhen University, Shenzhen 518060, P.R.China
2) Department of Health Science, McMaster University, Hamilton L8S4L8, Canada
关键词:
植物细胞学; 处理小体; 多核糖体; 脱帽酶1; 逆境胁迫; 信使核糖核酸; 烟草悬浮细胞-BY-2; 青色荧光蛋白
Keywords:
plant cytology;  processing body;  polysome;  decapping enzyme（DCP1）;  stress condition;  mRNA;  tobacco suspension cells-BY-2;  cyan fluorescent protein（CFP）
分类号:
Q 942.5；Q 256
DOI:
10.3724/SP.J.1249.2012.01080
文献标志码:
A
摘要:
指出处理小体（processing body，P-body）在真核细胞基因表达调节过程中，特别是在逆境条件下，能为mRNA在细胞质内转运提供储存和降解场所．研究通过农杆菌转化法成功将含有处理小体荧光标记物-脱帽酶1（decapping enzyme，DCP1）与青色荧光蛋白（cyan fluorescent protein，CFP）融合蛋白基因的植物表达载体转入烟草悬浮细胞．利用激光共聚焦显微镜观察到在高温和盐胁迫下，处理小体的数量和体积都有增加．通过蔗糖密度梯度离心分析逆境条件下多核糖体的变化，发现在高温和盐胁迫下，多核糖体解体，呈现翻译抑制特征．
Abstract:
Processing bodies(P-body) serve as key functions in regulating gene expression in eukaryotic cells, and especially under stress conditions, serve as stress-induced mRNA relocalization sites for mRNA degradation or storage. We successfully transformed P-body fluorescent marker (DCP1 and CFP fusion protein) containing plasmid into suspension BY-2 cells. Confocal observation found that P-body increased in abundance and size under heat and salt stress conditions. Sucrose gradient ultra-centrifugation analysis demonstrated that both heat and salt stress conditions induced polysome run-off. This is characteristic of inhibition of translation initiation.

参考文献/References:

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备注/Memo

备注/Memo:
Received：2011-08-25；Accepted：2011-11-01
Foundations：National Natural Science Foundation of China（30970265）, Natural Science Foundation of Guangdong Province（9151806001000017）
Corresponding author：Professor MO Bei-xin. E-mail: bmo@szu.edu.cn
Citation：MO Bei-xin, YE Hao, OU Zhong-hua, et al. Stress-induced processing body formation in tobacco suspension cells［J］. Journal of Shenzhen University Science and Engineering, 2012, 29(1): 80-84.(in Chinese)
基金项目：国家自然科学基金资助项目（30970265）；广东省自然科学基金自由申请项目基金资助（9151806001000017）
作者简介：莫蓓莘（1966-），女（汉族），江西省修水县人，深圳大学教授. E-mail: bmo@szu.edu.cn
引文：莫蓓莘，叶浩，欧忠华，等. 逆境诱导烟草悬浮细胞内处理小体的形成［J］. 深圳大学学报理工版，2012, 29(1)：80-84.
更新日期/Last Update: 2012-01-06