[1]莫蓓莘,叶浩,欧忠华,等.逆境诱导烟草悬浮细胞内处理小体的形成[J].深圳大学学报理工版,2012,29(No.1(001-094)):80-84.[doi:10.3724/SP.J.1249.2012.01080]
 MO Bei-xin,YE Hao,OU Zhong-hua,et al.Stress-induced processing body formation in tobacco suspension cells[J].Journal of Shenzhen University Science and Engineering,2012,29(No.1(001-094)):80-84.[doi:10.3724/SP.J.1249.2012.01080]
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逆境诱导烟草悬浮细胞内处理小体的形成()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第29卷
期数:
2012年No.1(001-094)
页码:
80-84
栏目:
生物工程
出版日期:
2012-01-06

文章信息/Info

Title:
Stress-induced processing body formation in tobacco suspension cells
作者:
莫蓓莘1叶浩1欧忠华1刘丽1黄彪1朱双琳12
1) 深圳大学生命科学学院,深圳 518060
2) 麦克马斯特大学健康科学系,加拿大 哈密尔顿 L8S4L8
Author(s):
MO Bei-xin1 YE Hao1 OU Zhong-hua1 LIU Li1 HUANG Biao1 and ZHU Shuang-lin12
1) College of Life Science, Shenzhen University, Shenzhen 518060, P.R.China
2) Department of Health Science, McMaster University, Hamilton L8S4L8, Canada
关键词:
植物细胞学处理小体多核糖体脱帽酶1逆境胁迫信使核糖核酸烟草悬浮细胞-BY-2青色荧光蛋白
Keywords:
plant cytology processing body polysome decapping enzyme(DCP1) stress condition mRNA tobacco suspension cells-BY-2 cyan fluorescent protein(CFP)
分类号:
Q 942.5;Q 256
DOI:
10.3724/SP.J.1249.2012.01080
文献标志码:
A
摘要:
指出处理小体(processing body,P-body)在真核细胞基因表达调节过程中,特别是在逆境条件下,能为mRNA在细胞质内转运提供储存和降解场所.研究通过农杆菌转化法成功将含有处理小体荧光标记物-脱帽酶1(decapping enzyme,DCP1)与青色荧光蛋白(cyan fluorescent protein,CFP)融合蛋白基因的植物表达载体转入烟草悬浮细胞.利用激光共聚焦显微镜观察到在高温和盐胁迫下,处理小体的数量和体积都有增加.通过蔗糖密度梯度离心分析逆境条件下多核糖体的变化,发现在高温和盐胁迫下,多核糖体解体,呈现翻译抑制特征.
Abstract:
Processing bodies(P-body) serve as key functions in regulating gene expression in eukaryotic cells, and especially under stress conditions, serve as stress-induced mRNA relocalization sites for mRNA degradation or storage. We successfully transformed P-body fluorescent marker (DCP1 and CFP fusion protein) containing plasmid into suspension BY-2 cells. Confocal observation found that P-body increased in abundance and size under heat and salt stress conditions. Sucrose gradient ultra-centrifugation analysis demonstrated that both heat and salt stress conditions induced polysome run-off. This is characteristic of inhibition of translation initiation.

参考文献/References:

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备注/Memo

备注/Memo:
Received:2011-08-25;Accepted:2011-11-01
Foundations:National Natural Science Foundation of China(30970265), Natural Science Foundation of Guangdong Province(9151806001000017)
Corresponding author:Professor MO Bei-xin. E-mail: bmo@szu.edu.cn
Citation:MO Bei-xin, YE Hao, OU Zhong-hua, et al. Stress-induced processing body formation in tobacco suspension cells[J]. Journal of Shenzhen University Science and Engineering, 2012, 29(1): 80-84.(in Chinese)
基金项目:国家自然科学基金资助项目(30970265);广东省自然科学基金自由申请项目基金资助(9151806001000017)
作者简介:莫蓓莘(1966-),女(汉族),江西省修水县人,深圳大学教授. E-mail: bmo@szu.edu.cn
引文:莫蓓莘,叶浩,欧忠华,等. 逆境诱导烟草悬浮细胞内处理小体的形成[J]. 深圳大学学报理工版,2012, 29(1):80-84.
更新日期/Last Update: 2012-01-06