TANG Yu-lin,CAO Yan,OU Zhong-hua,et al.Regulatable gene expression controlled by the promoter of Sali3-2 under different abiotic stresses[J].Journal of Shenzhen University Science and Engineering,2012,29(No.1(001-094)):73-79.[doi:10.3724/SP.J.1249.2012.01073]





Regulatable gene expression controlled by the promoter of Sali3-2 under different abiotic stresses
1) 深圳大学生命科学学院,深圳 518060
2) 深圳市海洋生物资源与生态环境重点实验室,深圳市微生物基因工程重点实验室,深圳 518060
TANG Yu-lin12 CAO Yan12 OU Zhong-hua12 YANG Xi-ni12 HU Lian-ping12 and ZHENG Yi-zhi12
1) College of Life Science, Shenzhen University, Shenzhen 518060, P.R.China
2) The Key Laboratory for Marine Bioresource and Eco-environmental Science, Shenzhen Key Laboratory of Microbial and Gene Engineering, Shenzhen 518060, P.R.China
bioinformatics gene regulation promoter Sali3-2 copper response elements abiotic stress GUS analysis
Q 78; Q 812
通过生物信息学方法对Sali3-2基因上游非编码区序列(-1 945 /+1)中的顺式作用元件进行预测.发现该序列中存在铜响应元件、干旱胁迫响应元件及与低温等非生物胁迫相关元件等.在不同非生物胁迫条件下,分别对转基因烟草悬浮细胞和转基因拟南芥幼苗中Sali3-2启动子片段驱动报告基因β-葡萄糖苷酸酶(β-glucuronidase,Gus)的表达进行分析.结果表明,在转基因烟草细胞和拟南芥幼苗中, Gus基因的表达明显受Al3+胁迫的诱导和Cu2+过多的抑制;在转基因烟草细胞中,该基因的表达一定程度上受脱落酸、渗透胁迫和盐胁迫的诱导,研究结果为了解Sali3-2基因的功能及调控作用奠定了基础.
Sali3-2 from soybean is a BURP gene related to stress response. To elucidate its transcriptional regulation characteristics, the upstream non-coding sequence (-1 945 /+1) of Sali3-2 was scanned in PLACE database for searching the cis-acting regulatory elements. Several copper response elements and some cis-element motifs related to gene induction by abscisic acid (ABA), dehydration and cold stress were found in this sequence. The expression of reporter gene Gus driven by the promoter region of Sali3-2 was further studied in tobacco cells and Arabidopsis seedlings at different abiotic stresses. Results show that the expression of the reporter is obviously up-regulated by Al3+ stress and intensely suppressed by abundance Cu2+. The expression of the reporter is slightly up-regulated by abscisic acid, osmotic stress and salt stress in transgenic tobacco cells. These findings extend our understanding of the regulation of Sali3-2 expression.


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Foundation:National Natural Science Foundation of China (30770184)
Corresponding author:Professor ZHENG Yi-zhi. E-mail: yzzheng@szu.edu.cn
Citation:TANG Yu-lin, CAO Yan, OU Zhong-hua, et al. Regulatable gene expression controlled by the promoter of Sali3-2 under different abiotic stresses[J]. Journal of Shenzhen University Science and Engineering, 2012, 29(1):73-79.(in Chinese)
作者简介:唐玉林(1968-),女(汉族),广西壮族自治区桂林市人,深圳大学副教授、博士. E-mail: yltang@szu.edu.cn
引文:唐玉林, 曹雁, 欧忠华, 等. 非生物胁迫因子对大豆Sali3-2基因的调控作用[J]. 深圳大学学报理工版,2012,29(1):73-79.
更新日期/Last Update: 2012-01-06