[1]尹忠平,上官新晨,米丽雪,等.青钱柳细胞悬浮培养及三萜化合物积累[J].深圳大学学报理工版,2011,28(No.5(377-470)):430-435.
 YIN Zhong-ping,SHANGGUAN Xin-chen,MI Li-xue,et al.Suspension culture of Cyclocarya paliurus cells and accumulation of triterpenoids[J].Journal of Shenzhen University Science and Engineering,2011,28(No.5(377-470)):430-435.
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青钱柳细胞悬浮培养及三萜化合物积累()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第28卷
期数:
2011年No.5(377-470)
页码:
430-435
栏目:
生物工程
出版日期:
2011-09-20

文章信息/Info

Title:
Suspension culture of Cyclocarya paliurus cells and accumulation of triterpenoids
文章编号:
1000-2618(2011)05-0430-06
作者:
尹忠平12上官新晨12米丽雪2蒋艳2吴少福2张月红2
1)食品科学与技术国家重点试验室,南昌大学,南昌 330047
2)江西省教育厅天然产物研究与开发重点实验室,江西农业大学,南昌 330045
Author(s):
YIN Zhong-ping12SHANGGUAN Xin-chen12MI Li-xue2JIANG Yan2WU Shao-fu2and ZHANG Yue-hong2
1)State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P.R.China
2)Jiangxi Education Department Key Laboratory of Natural Resources Exploitation & Utilization, Jiangxi Agricultural University, Nanchang 330045, P.R.China
关键词:
细胞工程植物细胞培养薄层层析高效液相色谱代谢产物青钱柳三萜化合物
Keywords:
cell engineeringplant cell culturethin layer chromatographyhigh performance liquid chromatographymetabolitesCyclocarya paliurustriterpenoids
分类号:
Q 813.1
文献标志码:
A
摘要:
研究青钱柳细胞悬浮培养体系的建立及三萜化合物的积累.结果表明,以青钱柳无菌幼叶为外植体,可诱导出愈伤组织.选择绿色、较为疏松的愈伤组织继续筛选和继代培养,可获得疏松易碎、生长旺盛、适于悬浮培养的优良愈伤组织.青钱柳细胞优化培养条件为:MS培养基、2,4-D 0.8 mg/L、NAA 1.8 mg/L、6-BA 0.8 mg/L、KT 0.8 mg/L、蔗糖40 g/L,pH值6.0,100 mL三角瓶中装液量为40 mL,接种量为3 g(鲜质量),暗培养.该条件下悬浮细胞生长迅速,最大生长量(干质量)为15.56 g/L,总三萜产量达1 179.85 mg/L,熊果酸和齐墩果酸是其中主要的三萜化合物,产量分别达443.64 mg/L和159.00 mg/L.青钱柳细胞悬浮培养可用于熊果酸和齐墩果酸生产.
Abstract:
In order to provide references for industrialized production of secondary metabolites such as triterpenoids by plant cell culture,the establishment of Cyclocarya paliurus cell suspension culture system and the accumulation of triterpenoids were studied.Results show that callus could be induced from sterile immature leaves.The crisp green callus was further selected for subculture and suspension culture.Suspended cells grew very well.The increased dry weight of cells could reach a level of 15.56 g/L under the following conditions:MS media containing 2,4-D 0.8 mg/L,NAA 1.8 mg/L,6-BA 0.8 mg/L,KT 0.8 mg/L and 4 % sucrose,pH=6.0,inoculum concentration (fresh weight) of 3 g/40 mL,filling volume of 40 mL/100 mL,dark cultivation.The output of total triterpenoids was up to 1 179.85 mg/L,ursolic acid and oleanolic acid were the major compounds of total triterpenoids,and its output reached 443.64 mg/L and 159.00 mg/L under the optimal culture conditions.These results suggest that Cyclocarya paliurus cell suspension culture could be applied to producing ursolic acid and oleanolic acid.

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备注/Memo

备注/Memo:
收稿日期:2010-10-17;修回日期:2011-03-22
基金项目:国家自然科学基金资助项目(31060213);江西省科技支撑计划资助项目(2009BGA01900)
作者简介:尹忠平(1971-),男(汉),湖南省炎陵县人,南昌大学博士研究生.E-mail:yinzp2008@yahoo.com.cn
通讯作者:上官新晨(1962-),男(汉),南昌大学教授、博士生导师.E-mail:shangguanxc_818@sina.com
更新日期/Last Update: 2011-09-23