[1]王静凤,傅佳,徐雷雷,等.日本刺参降血糖及对胰岛素信号通路的影响[J].深圳大学学报理工版,2011,28(No.2(095-188)):172-177.
 WANG Jing-feng,FU Jia,XU Lei-lei,et al.Involvement of the PI3K/Akt pathway in the hypoglycemic effects of sea cucumber Apostichopus japonicas in diabetic rats[J].Journal of Shenzhen University Science and Engineering,2011,28(No.2(095-188)):172-177.
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日本刺参降血糖及对胰岛素信号通路的影响()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第28卷
期数:
2011年No.2(095-188)
页码:
172-177
栏目:
生物工程
出版日期:
2011-03-20

文章信息/Info

Title:
Involvement of the PI3K/Akt pathway in the hypoglycemic effects of sea cucumber Apostichopus japonicas in diabetic rats
文章编号:
1000-2618(2011)02-0172-06
作者:
王静凤傅佳徐雷雷杨延存王玉明薛长湖
中国海洋大学食品科学与工程学院, 青岛 266003
Author(s):
WANG Jing-fengFU JiaXU Lei-leiYANG Yan-cunWANG Yu-mingXUE Chang-hu
College of Food Science and Engineering,Ocean University of China,Qingdao 266003,P.R.China
关键词:
胰岛素糖尿病信号转导磷脂酰肌醇-3-激酶蛋白激酶B葡萄糖转运蛋白4中药药理学日本刺参
Keywords:
insulindiabetessignal transduction phosphatidylinositol 3 kinase(PI3K)protein kinase BPKBglucose transporter 4(GLUT4)pharmacology of traditional Chinese medicineApostichopus japonicas
分类号:
R 285.5
文献标志码:
A
摘要:
以腹腔注射链脲佐菌素建立糖尿病大鼠模型,用日本刺参(Apostichopus japonicas)灌胃糖尿病大鼠35 d,观察其对大鼠空腹血糖、葡萄糖耐量和血清胰岛素的影响,并对其作用机制进行探讨.采用逆转录聚合酶链式反应(reverse transcription polymerase chain reaction,RT-PCR)技术检测糖尿病大鼠骨骼肌和脂肪组织中磷脂酰肌醇-3-激酶(phosphatidylinositol 3 kinase,PI3K)、蛋白激酶B(protein kinase B,PKB)和葡萄糖转运蛋白4(glucose transporter,GLUT4)等基因mRNA表达水平,Western blot方法检测GLUT4蛋白表达水平.结果显示,日本刺参能显著降低糖尿病大鼠空腹血糖含量,提高口服葡萄糖耐量和血清胰岛素含量;提高骨骼肌和脂肪组织中PI3K、PKB和Glut4 mRNA表达量,促进脂肪组织中GLUT4蛋白表达.日本刺参对糖尿病大鼠脂肪组织PKB mRNA表达和骨骼肌GLUT4蛋白表达无显著性影响.提示日本刺参具有较好的降血糖作用,其作用机制与部分上调胰岛素介导的PI3K/PKB/Glut4信号转导通路有关.
Abstract:
This was to study the effect of sea cucumber Apostichopus japonicus treatment on fasting plasma glucose,glucose tolerance,serum insulin level and insulin signaling pathway in streptozotocin(STZ)-induced diabetic rats.The diabetic rat model was generated by injection intraperitoneally with STZ (60 mg/kg).The diabetic rats were divided into two groups:the model control group and sea cucumber group.Rats in sea cucumber group were administered orally with A.japonicus(800 mg/kg).The other rats were given with 0.9% NaCl solution as placebo.All agents were administered once a day for 35 days.Changes in blood glucose level,oral glucose tolerance and insulin secretion index were routinely evaluated.The expressions of phosphatidylinositol 3 kinase(PI3K),protein kinase B(PKB) and glucose transporter 4(GLUT4) in skeletal muscle and adipose tissues were determined with reverse transcription PCR (RT-PCR) or Western blot.The results show that A.japonicas can reduce the fasting plasma glucose,improve glucose tolerance and serum insulin contents in diabetic rats significantly.RT-PCR and Western blotting analysis revealed that A.japonicas treatment caused a significant increase in the expression levels of PI3K,PKB and Glut4 mRNA in both skeletal muscle and adipose tissues,and GLUT4 protein in adipose tissues,whereas there was no significant change in the expression of GLUT4 protein in skeletal muscle tissue compared with the diabetic control rats.These results indicate that A.japonicas is beneficial for improving hyperglycemia by enhancing part of the signal transduction pathway of PI3K/PKB/Glut4 in insulin sensitive skeletal muscle and adipose tissues.

参考文献/References:

[1]赵芹,王静凤,薛勇,等.3 种海参的主要活性成分和免疫调节作用的比较研究[J].中国水产科学,2008,15(1):154-158.
[2]王静凤,王玉明,唐庆娟.低分子量的日本刺参胶原肽的抗氧化活性[J].中国海洋大学学报,2010,9(1):1-10.(英文版)
[3]樊绘曾.关于海参及其成分保健医疗功能的研究与开发[J].中国海洋药物.2001,4(82):37-44.
[4]LIU Min,WU Ke,MAO Xian-qing,等.黄芪多糖提高KKAy小鼠胰岛素敏感性:对骨骼肌中PKB/GLUT4信号通路的调节[J].民族药理学杂志,2010,127(1):32-37.(英文版)
[5]Cho J Y,Park J.天然抑制剂对PI3K/PDK1/PKB信号通路中胰岛素介导的细胞内信号级联效应的影响[J].国际分子科学杂志,2008,9(11):2217-2230.(英文版)
[6]Tirosh A,Potashnik R,Bashan N,等.氧化应激扰乱了胰岛素诱导的胰岛素受体底物-1和PI3K在3T3-L1脂肪细胞内的重新分布[J].生物化学杂志,1999,274(15):10595-10602.(英文版)
[7]Pessler D,Rudich A,Bashan N.氧化应激损伤核蛋白结合GLUT4 启动子的胰岛素反应元件[J].糖尿病学,2001,44(12) :2156-2164.(英文版)
[8]王静凤,逄龙,王玉明,等.日本刺参酸性粘多糖、皂苷和胶原蛋白多肽对血管内皮细胞的保护作用[J].中国药理学通报,2008,24(2):227-232.
[9]王雪松,郑芸,方积年.降血糖多糖及寡糖的研究进展[J].药学学报,2004,39(12):1028-1032.
[10]王雨秾,孙佳犄,刘毓敏.人参皂苷对胰岛素抵抗大鼠模型中GLUT4和PI3K表达的影响[J].辽宁中医药大学学报,2009,11(6):234-237.



[1]ZHAO Qin,WANG Jing-feng,XUE Yong,et al.Comparative study on the bioactive components and immune function of three species of sea cucumber[J].Journal of Fishery Sciences of China,2008,15(1):154-158.(in Chinese)
[2]WANG Jing-feng,WANG Yu-ming,TANG Qing-juan,et al.Antioxidant activities of Low-molecular-weight gelatin hydrolysate derived from the sea cucumber Stichopus japonicus[J].Journal of Ocean University of China.2010,9(1):1-10.
[3]FAN Hui-zeng.The research and development of sea cucumber and its compositions in medical function[J].Chinese Journal of Marine Drugs.2001,4(82):37-44.(in Chinese)
[4]LIU Min,WU Ke,MAO Xian-qing,et al.Astragalus polysaccharide improves insulin sensitivity in KKAy mice:regulation of PKB/GLUT4 signaling in skeletal muscle[J].Journal of Ethnopharmacology,2010,127(1):32-37.
[5]Cho J Y,Park J.Contribution of natural inhibitor to the understanding of the PI3K/PDK1/PKB pathway in the insulin-medeated intracellular signaling cascade[J].International Journal of Molecular Sciences,2008,9(11):2217-2230.
[6]Tirosh A,Potashnik R,Bashan N,et al.Oxidative stress disrupts insulin-induced cellular redistribution of insulin receptor substrate-1and phosphatidylinositol 3-kinase in 3T3-L1 adipocytes[J].The Journal of biological chemistry,1999,274(15):10595-10602.
[7]Pessler D,Rudich A,Bashan N.Oxidative stress impairs nuclear proteins binding to the insulin respontive element in the GLUT4 promoter[J].Diabetologia,2001,44(12):2156-2164.
[8]WANG Jing-feng,PANG Long,WANG Yu-ming,et al.Protective effects of polysaccharides,triterpene glycosides and collagen polypeptides from Apostichopus japonicus on vascular endothelial cells[J].Chinese Pharmacological Bulletin,2008,24(2):227-232.(in Chinese)
[9]WANG Xue-song,ZHENG Yun,FANG Ji-nian.Recent advance in the study on poly-and oligo-saccharides with hypoglycemic activity[J].Acta Pharmaceutica Sinica,2004,39(12):1028-1032.(in Chinese)
[10]WANG Yu-nong,SUN Jia-ji,LIU Yu-min.Effect of expression by ginsenoside to GLUT4 and PI3K to insulin resistance of rat model[J].Journal of Liaoning University of Traditional Chinese Medicine,2009,11(6):234-237.(in Chinese)

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备注/Memo

备注/Memo:
收稿日期:2010-05-31;修回日期:2010-11-09
基金项目:国家自然科学基金资助项目 (30972284 );海洋公益性行业科研专项资金资助项目(201105029)
作者简介:王静凤(1964-),女(汉族),山东省荣成市人,中国海洋大学教授、博士生导师.E-mail:jfwang@ouc.edu.cn
通讯作者:薛长湖(1964-),男(汉族),中国海洋大学教授、博士生导师.E-mail:xuech@ouc.edu.cn
更新日期/Last Update: 2011-03-28