[1]王海滔,刘剑洪,张黔玲,等.钌(Ⅱ)配合物光谱性质及对DNA光切割作用[J].深圳大学学报理工版,2011,28(No.1(001-095)):47-53.
 WANG Hai-tao,LIU Jian-hong,ZHANG Qian-ling,et al.DNA-binding spectrum and DNA-photocleavage studies of ruthenium complex [Ru(bpy)2(MDBIP)]2+[J].Journal of Shenzhen University Science and Engineering,2011,28(No.1(001-095)):47-53.
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钌(Ⅱ)配合物光谱性质及对DNA光切割作用()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第28卷
期数:
2011年No.1(001-095)
页码:
47-53
栏目:
化学与化工
出版日期:
2011-01-10

文章信息/Info

Title:
DNA-binding spectrum and DNA-photocleavage studies of ruthenium complex [Ru(bpy)2(MDBIP)]2+
文章编号:
1000-2618(2011)01-0047-07
作者:
王海滔1刘剑洪1张黔玲1胡婷婷1田生礼2
1) 深圳大学化学与化工学院 深圳市功能高分子重点实验室,深圳518060
2) 深圳大学生命科学学院,深圳518060
Author(s):
WANG Hai-tao1LIU Jian-hong1ZHANG Qian-ling1HU Ting-ting1and TIAN Sheng-li2
1)College of Chemistry and Chemical Engineering,Shenzhen Key Laboratory of Functional Polymer,Shenzhen University, Shenzhen 518060,P.R.China
2)College of Life Science,Shenzhen University,Shenzhen 518060,P.R.China
关键词:
生物无机化学 脱氧核糖核酸 多吡啶钌配合物 小牛胸腺DNApBR322质粒DNA光切割
Keywords:
bioinorganic chemistrydeoxyribonucleic acid(DNA) polypyridyl ruthenium complexcalf thymus DNApBR322 plasmid DNAphoto-cleavage
分类号:
O 614.82+1;Q 523
文献标志码:
A
摘要:
用紫外吸收光谱、荧光光谱和荧光淬灭技术,研究钌(Ⅱ)多吡啶配合物 [Ru(bpy)2(MDBIP)]2+ (bpy为2,2′-联吡啶; MDBIP为3,5-二溴基-咪唑并[4,5-f][1,10]邻菲咯啉)与小牛胸腺DNA的作用.用琼脂糖凝胶电泳法研究配合物对pBR322质粒DNA的光切割作用.结果表明,[Ru(bpy)2(MDBIP)]2+与小牛胸腺DNA可能是以一种弱的部分插入的方式相结合,对pBR322质粒DNA有较好的光切割作用.
Abstract:
The binding properties of a new ruthenium(Ⅱ) complex [Ru(bpy)2(MDBIP)]2+(bpy:2,2′-dipyridine,MDBIP:3,5-dibromo-imidazo[4,5-f][1,10]phenanthroline) with calf thymus DNA were studied by UV-visible absorption spectroscopy and fluorescent spectroscopy.Agarose gel electrophoresis was used to investigate the binding and photo-cleavage of pBR322 plasmid DNA with the complex.Results indicated that [Ru(bpy)2(MDBIP)]2+ bound to calf thymus DNA by partial intercalation and had a good photo-cleavage effect on pBR322 plasmid DNA.

参考文献/References:




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备注/Memo

备注/Memo:
收稿日期:2010-03-11;修回日期:2010-05-24
基金项目:国家自然科学基金资助项目(20601019)
作者简介:王海滔(1975-),男(汉族),浙江省台州市人,深圳大学讲师.E-mail:wanght2000cn@yahoo.com.cn
通讯作者:刘剑洪(1962-),男(汉族),深圳大学教授、博士生导师.E-mai:szujhliu@yahoo.com.cn
更新日期/Last Update: 2011-01-13