[1]包木太,袁书文,李希明,等.多孔介质渗透率对油藏微生物生长代谢影响[J].深圳大学学报理工版,2011,28(No.1(001-095)):35-40.
 BAO Mu-tai,YUAN Shu-wen,LI Xi-ming,et al.Effects of permeability of porous medium on the growth and metabolism of microorganism in reservoir[J].Journal of Shenzhen University Science and Engineering,2011,28(No.1(001-095)):35-40.
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多孔介质渗透率对油藏微生物生长代谢影响()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第28卷
期数:
2011年No.1(001-095)
页码:
35-40
栏目:
环境与能源
出版日期:
2011-01-10

文章信息/Info

Title:
Effects of permeability of porous medium on the growth and metabolism of microorganism in reservoir
文章编号:
1000-2618(2011)01-0035-06
作者:
包木太12袁书文12李希明3宋智勇3郭辽源3
1) 中国海洋大学海洋化学理论与工程技术教育部重点实验室,青岛 266100
2) 中国海洋大学化学化工学院,青岛 266100
3) 中国石化胜利油田分公司采油工艺研究院,山东 东营 257000
Author(s):
BAO Mu-tai12YUAN Shu-wen12LI Xi-ming3SONG Zhi-yong3and GUO Liao-yuan3
1)Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education,Ocean University of the People’s Republic of China,Ocean University of China,Qingdao 266100,P.R.China
2)College of Chemistry & Chemical Engineering,Ocean University of China,Qingdao 266100,P.R.China
3)Research institute of oil production technology,Shengli Oilfield Company,Sinopec,Shandong Prouince,Dongying 257000,P.R.China
关键词:
采油工程 环境微生物 油藏 渗透率 内源微生物采油 聚合酶链反应-变性梯度凝胶电泳
Keywords:
oil well productionenvironmental microorganismreservoirpermeabilityindigenous microorganismspolymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE)
分类号:
X 172;TE 357.9
文献标志码:
A
摘要:
采油微生物在提高原油采收率过程中,受油藏环境条件,包括温度、压力、矿化度、pH值和渗透率等因素影响.用人工填沙岩心模拟地层多孔介质,以淀粉水解液为主要碳源,通过对胜利油田中一区Ng3产出水中的内源微生物,在60 ℃高温和10 MPa高压下的静态激活实验,考察在不同渗透率的多孔介质中,采油微生物的激活代谢情况.利用聚合酶链反应-变性梯度凝胶电泳(polymerase chain reaction-denaturing gradient gel electrophoresis,PCR-DGGE)技术,研究不同渗透率岩心激活后微生物群落DGGE条带的变化.结果表明,微生物在不同渗透率多孔介质中的生长代谢有较大差异,岩心渗透率显著影响微生物群落结构,高渗透多孔介质培养条件下微生物种类比低渗透介质条件下多,丰度也较高.
Abstract:
There are many environmental factors,such as temperature,pressure,salinity,pH,and permeability, affecting microbial enhanced oil recovery (MEOR).The microbial metabolism in various porous media permeability under high temperature (60 ℃) and high pressure (10 MPa) was investigated.The strata in porous media was simulated by sand packed column.The in-situ microorganism of produced water from Zhongyi block in Shengli Oil Field was cultured in the sand packed column with starch hydrolysates,which served as the major carbon source.The changes of microbial community in different permeability activation were explored by PCR-DGGE technique.The results showed that the growth and metabolism of microorganism varied with different permeability of porous media,and the core permeability significantly affected the microbial community structure.The species and abundance of microorganisms in high permeability porous media were more than those in low permeability porous media.

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备注/Memo

备注/Memo:
收稿日期:2010-07-19;修回日期:2010-10-09
基金项目:国家自然科学基金资助项目(50604013)
作者简介:包木太(1971-),男(汉族),山东省临沂市人,中国海洋大学教授、博士生导师.E-mail:mtbao@ouc.edu.cn
更新日期/Last Update: 2011-01-13