[1]邵永红,李恒,王岩,等.基于同步扫描相机的荧光寿命测量系统研究[J].深圳大学学报理工版,2009,26(4):331-336.
 SHAO Yong-hong,LI Heng,WANG Yan,et al.A fluorescence lifetime spectrometer based on a synchroscan streak camera[J].Journal of Shenzhen University Science and Engineering,2009,26(4):331-336.
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基于同步扫描相机的荧光寿命测量系统研究()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第26卷
期数:
2009年4期
页码:
331-336
栏目:
生物医学工程
出版日期:
2009-10-31

文章信息/Info

Title:
A fluorescence lifetime spectrometer based on a synchroscan streak camera
文章编号:
1000-2618(2009)04-0331-06
作者:
邵永红1李恒12王岩1屈军乐1牛憨笨1
1)深圳大学光电工程学院,光电子器件与系统教育部重点实验室,广东省光电子器件与系统重点实验室,深圳 518060;
2)西安工业大学光电工程学院,西安 710032
Author(s):
SHAO Yong-hong1LI Heng12WANG Yan1QU Jun-le1and Hanben NIU1
1)College of Optoelectronic Engineering,Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education,Key Laborotory of Optoelectronics Devices and Systems of Guangdong Province,Shenzhen University,Shenzhen 518060,P.R.China
2)College of Opt-electro Engineering,Xi’an Technological University,Xi’an 710032,P.R.China
关键词:
荧光显微技术荧光寿命双光子激发同步扫描相机扫描速度时间分辨率
Keywords:
fluorescence microscopyfluorescence lifetimetwo-photon excitationsynchroscan streak camerasweep speedtemporal resolution
分类号:
TB 872;O 43;Q 631
文献标志码:
A
摘要:
建立一台基于同步扫描相机的双光子激发荧光寿命显微测量系统,同步扫描相机的重复工作频率为76 MHz,利用钛宝石飞秒激光器作为光源,通过可调延时器和标准具对扫描相机的时间分辨率、扫描速度以及非线性等进行标定.该系统的时间分辨率为9 ps,非线性小于4%,量程为2.8 ns.测量了荧光染料Rose Bengal(RsB)的荧光衰减曲线,通过最小二乘法对荧光的衰减曲线进行拟合,得到RsB的荧光寿命为763 ps,与标准荧光染料对比一致.
Abstract:
A two-photon excitation fluorescence lifetime spectrometer based on a synchroscan streak camera was presented.The spectrometer was calibrated using a Titanium:Sapphire femtosecond laser in conjunction with a custom-made synchronized streak circuit,tunable delay generator and a set of etalons.Experimental results show that the temporal resolution of this spectrometer is 9 ps,nonlinearity is less than 4%,and the sweep range is 2.8 ns.Fluorescence decay profile of fluorescent dye Rose Bengal (RsB) is recorded with the spectrometer.Nonlinear least square fitting of the decay profile gives that the lifetime of RsB is 763 ps,consistent with standard fluorescence dyes.

参考文献/References:

[1]屈军乐.荧光寿命成像显微技术的理论与实验研究[D].西安:中国科学院西安光学精密机械研究所,1998.
[2]刘立新.荧光多参量显微技术研究[D].西安:中国科学院西安光学精密机械研究所,2006.
[3]Becker W,Bergmann A,Biskup C,等.高分辨率TCSPC荧光寿命成像技术[C]//生物医学科学中的多光子显微会议论文集Ⅲ.圣荷塞(美国):国际光学工程学会.2003(4963):175-184(英文版)
[4]Cubeddu R,Comelli D,D′Andrea C,等.生物医学中时间分辨荧光成像技术[J].物理学D辑:应用物理,2002,35(9):61-76(英文版).
[5]刘立新,屈军乐,林子扬,等.双光子激发时间分辨荧光光谱测量技术[J].物理学报,2006,55(12):6281-6286.
[6]Buist A H,Muller M,Squier J,等.用于多点激发的实时双光子显微系统[J].显微系统杂志,1998,192(2):217-226(英文版).
[7]Denk W,Strickler J H,Webb W W.双光子激光扫描荧光显微系统 [J].科学,1990,248(4951):73-76(英文版).
[8]牛丽红,杨勤劳,牛憨笨,等.宽动态范围X射线扫描相机系统[J].科学仪表,2008,79:023103(英文版).
[9]FANG Qi-yin,Papaioannou T,Jo J A,等.用于临床诊断的时域激光诱导荧光光谱仪[J].科学仪器,2004,75(1):151-162.
[10]屈军乐,李冀,牛憨笨. 基于同步扫描相机的荧光寿命成像显微技术 [C]//三维与多维显微镜图像捕获与处理XI.圣荷塞(美国):国际光学工程学会,2004,5324(5):127-129(英文版).
[11]刘立新,屈军乐,林子扬,等.荧光寿命成像及其在生物医学中的应用[J].深圳大学学报理工版,2005,22(2):133-141.
[12]刘立新,屈军乐,林子扬,等.用于双光子激发荧光寿命显微成像的高重复频率皮秒扫描相机[J].光学学报,2006,26(3):373-378.
[13]Krishnan R V,Saitoh H,Terada H,等.用于扫描相机的多光子荧光寿命成像显微系统的进展[J].科学仪表,2003,74(5):2714-2721(英文版).
[14]刘永刚,彭晓世,汤小青.光学条纹相机扫描速度和非线性的测试[J].核电子学与探测技术,2004,24(2):218-220.
[15]Pelet S,Previte M,Laiho L H,等.基于图像分割的荧光寿命成像显微的快速全局拟合算法研究[J].生物物理杂志,2004,87(4):2807-2817(英文版).
[16]Becker W,Bergmann A,Biscotti G,等.基于TCSPC的高速荧光寿命数据处理 [C]//生物医学科学中的多光子显微会议论文集Ⅳ.圣荷塞(美国):国际光学工程学会,2004,2532(5):27-35(英文版).
[17]Talbot C,Mcginty J,Mcghee E,等.利用时间门nipkow旋转盘或多交点多光时间相关单光子计数显微法进行高速光学层析荧光寿命成像技术 [C]//生物光学.圣彼得斯堡(美国):美国光学学会2008:BWD2(英文版).
[18]刘立新,屈军乐,王磊,等.五维多焦点多光子显微技术[C]//生物医学光学与图像.柏林翰(美国):国际光学工程学会,2006,8(1):65341Q.1-65341Q.9(英文版).
[19]屈军乐,刘立新,郭宝平,等.用于扫描相机多光谱多光子荧光寿命成像显微系统的进展 [C]//生物医学光学与卫生保健.北京: 国际光学工程学会, 2005,5630:510-526(英文版).

[1]QU Jun-le,Theoretical and experimental study of fluorescence lifetime imaging microscopy[D].Xi’an:Institute of Optics and Precision Mechanics,Chinese Academy of Science,1998(in Chinese).
[2]LIU Li-xin,Study of fluorescence multi-parameter microscopy[D].Xi’an:Institute of Optics and Precision Mechanics,Chinese Academy of Science,2006(in Chinese).
[3]Becker W,Bergmann A,Biskup C,et al.High resolution TCSPC lifetime imaging[C]//Multiphoton Microscopy in the Biomedical ScienceⅢ.San Jose(USA):SPIE 2003(4963):175-184.
[4]Cubeddu R,Comelli D,D′Andrea C,et al.Time-resolved fluorescence imaging in biology and medicine[J].Journal of Physics-D:Applied Physics,2002,35(9):61-76.
[5]LIU Li-xin,QU Jun-le,LIN Zi-yang,et al.Time-resolved two-photon excitation fluorescence spectroscopy [J].Acta Physica Sinica,2006,55(12):6281-6286(in Chinese).
[6]Buist A H,Muller M,Squier J,et al.Real time two-photon absorption microscopy using multi point excitation.[J].Journal of Microscopy,1998,192(2):217-226.
[7]Denk W,Strickler J H,Webb W W.Two-photon laser scanning fluorescence microscopy[J].Science,1990,248(4951):73-76.
[8]NIU Li-hong,YANG Qin-lao,Hanben NIU,et al.A wide dynamic range X-ray streak camera system[J].Review of Scientific Instruments,2008,79:023103.
[9]FANG Qi-yin,Papaioannou T,Jo J A,et al.Time-domain laser-induced fluorescence spectroscopy apparatus for clinical diagnostics[J].Review of Scientific Instruments,2004,75:151-162.
[10]QU Jun-le,LI Ji,Hanben NIU.Fluorescence lifetime imaging microscopy based on a synchroscan streak camera[C]//Conferenceon Three-Dimensional and Multidimensional Microscopy:Image Acquisition and Praessing XI.San Jose(USA):SPIE,2004,5324(5):127-129.
[11]LIU Li-xin,QU Jun-le,LIN Zi-yang,et al. Fluorescence lifetime imaging and its biomedical applications[J]. Journal of Shenzhen University Science and engineering, 2005,22(2):133-141.
[12]LIU Li-xin,QU Jun-le,LIN Zi-yang,et al.A high repetition rate picosecond streak camera for two-photon excited fluorescence lifetime microscopic imaging [J].Acta Optica Sinica,2006,26(3):373-378(in Chinese).
[13]Krishnan R V,Saitoh H,Terada H,et al.Development of a multiphoton fluorescence lifetime imaging microscopy system using a streak camera[J].Review of Scientific Instruments,2003,74(5):2714-2721.
[14]LIU Yong-gang,PENG Xiao-shi,TANG Xiao-qing.Measuring sweep speed and sweep nonlinearity of optic streak camera [J].Nuclear Electronics & Detection Technology,2004,24(2):218-220(in Chinese).
[15]Pelet S,Previte M,Laiho L H,et al.A fast global fitting algorithm for fluorescence lifetime imaging microscopy based on image segmentation[J].Biophysical Journal,2004,87(4):2807-2817.
[16]Becker W,Bergmann A,Biscotti G,et al.High-speed FLIM data acquisition by time-correlated single photon counting[C]//Conference on Multiphoton Microscopy in the Biomedical Sciences Ⅳ.San Jose(USA):SPIE,2532(5):27-35.
[17]Talbot C,Mcginty J,Mcghee E,et al.High speed,optically sectioned fluorescence lifetime imaging utilizing time-gated nipkow disk or multifocal multiphoton time correlated single photon counting microscopy[J]//Biomedical Optics.St Petersburg:Optical Society of America,2008:BWD2.
[18]LIU Li-xin,QU Jun-le,WANG Lei,et al.Five-dimensional multifocal multiphoton microscopy[C]//Progress in Biomedical Optics and Imaging.Belligham:Society of Photo-Optical Instrumentation Engineers,2006,8(1):65341Q.1-65341Q.9.
[19]QU Jun-le,LIU Li-xin,GUO Bao-ping,et al.Development of a multispectral multiphoton fluorescence lifetime imaging microscopy system using a streak camera[C]//Optics in Health Care and Biomedical Optics.Beijing:SPIE,2005,5630:510-526.

相似文献/References:

[1]刘立新,屈军乐,林子扬,等.荧光寿命成像及其在生物医学中的应用[J].深圳大学学报理工版,2005,22(2):133.
 LIU Li-xin,QU Jun-le,GUO Bao-ping,et al.Fluorescence lifetime imaging and its biomedical applications[J].Journal of Shenzhen University Science and Engineering,2005,22(4):133.

备注/Memo

备注/Memo:
收稿日期:2009-05-08;修回日期:2009-06-08
基金项目:国家自然科学基金资助项目( 60627003);广东省科技计划资助项目(2008a060205003)
作者简介:邵永红(1972-),男(汉族),吉林省德惠市人,深圳大学副教授、博士.E-mail:shaoyh@szu.edu.cn
通讯作者:牛憨笨(1940-),男(汉族),中国工程院院士、深圳大学研究员.E-mail:hbniu@szu.edu.cn
更新日期/Last Update: 2009-11-08