[1]郑承纲,俞理,吴庆红,等.一株烃降解菌Rhodococcus ruber Z25研究[J].深圳大学学报理工版,2009,26(3):234-239.
 ZHENG Cheng-gang,YU Li,WU Qing-hong,et al.Rhodococcus ruber Z25,a hydrocarbon-degradating strain[J].Journal of Shenzhen University Science and Engineering,2009,26(3):234-239.
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一株烃降解菌Rhodococcus ruber Z25研究()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第26卷
期数:
2009年3期
页码:
234-239
栏目:
环境与能源
出版日期:
2009-07-31

文章信息/Info

Title:
Rhodococcus ruber Z25,a hydrocarbon-degradating strain
文章编号:
1000-2618(2009)03-0234-06
作者:
郑承纲1俞理2吴庆红2黄立信2崔庆峰2
1)中国科学院渗流流体力学研究所,河北 廊坊 065007;
2)中国石油勘探开发研究院廊坊分院,河北 廊坊 065007
Author(s):
ZHENG Cheng-gang1 YU Li2 WU Qing-hong2 HUANG Li-xin2 and CUI Qing-feng2
1)Institute of Porous Flow & Fluid Mechanics,Chinese Academy of Sciences,Langfang 065007,Hebei,P.R.China
2)Research Institute of Petroleum Exploitation and Development,Langfang,China National Petroleum Corporation,Langfang 065007,Hebei,P.R.China
关键词:
石油污染环境修复烃降解菌Rhodococcus ruber 生物表面活性剂薄层层析气相色谱
Keywords:
oil pollutionenvironment modificationhydrocarbon-degradating bacteriaRhodococcus ruber biosurfactantthin layer chromatographygas chromatography
分类号:
X 172
文献标志码:
A
摘要:
烃降解菌株Z25分离自大庆油田中新201区块采油污水样,经形态观察和16S rDNA基因序列分析,鉴定为Rhodococcus ruber Z25.该菌株在20~45 ℃,0~5%盐的质量浓度下生长良好,适宜生长温度为30~40 ℃,最适盐的质量分数为2.5%.Rhodococcus ruber Z25菌株能以液体石蜡为唯一碳源生长并合成糖脂类生物表面活性剂,发酵48 h,细菌生物量和糖脂产量分别为1.53 g/L和13.22 g/L.经气相色谱对Rhodococcus ruber Z25菌株在好氧和厌氧条件下原油降解的全烃组分分析,结果表明:该菌株在好氧条件下优先降解石油中的轻烃组分,在厌氧条件下优先降解石油中的重烃组分.
Abstract:
A hydrocarbon-degradating strain was isolated from the oil production water of Zhongxin 201 Unit in Daqing Oilfield.The strain was identified to be Rhodococcus ruber Z25 by morphology and 16S rDNA sequencing.The strain was able to grow at a wide range of temperature (20~45 ℃) and salinity (0~5.0%)with the suitable growth condition being at NaCl concentration of 2.5% at 30~40 ℃.In batch fermentation,the strain could utilize liquid paraffin as sole carbon source and synthesize biosurfactant.The maximum biomass and biosurfactant production were obtained at 48 h with the values of 1.53 and 13.22 g/L,respectively.The biodegradation test of crude oil was conducted under aerobic and anaerobic conditions.The alkanes components in the crude oil were analyzed by gas chromatography.The test results showed that the strain Rhodococcus ruber Z25 had a preference of utilizing light components under aerobic condition and degradating heavy components under anaerobic condition.

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备注/Memo

备注/Memo:
收稿日期:2009-05-26;修回日期:2009-06-16
基金项目:国家重点基础研究发展计划资助项目(2005cb221308)
作者简介:郑承纲(1982-),男(汉族),天津市人,中国科学院博士研究生.E-mail:cg1982.zheng@gmail.com
通讯作者:俞理(1964-),男(汉族),中国石油勘探开发研究院高级工程师.E-mail:yuli3058@163.com
更新日期/Last Update: 2009-08-10